Chlorogenic acid is a bioactive phenolic acid compound found in a variety of medicinal materials and foods.As a secondary metabolic product in plant cells,chlorogenic acid has many pharmacological effects,such as antibacterial effect,immune regulation,anti-inflammatory,antiviral,anti-aging,anti-cardiovascular dis-ease,anti-cancer,regulation of blood glucose and lipids and so on.In recent years,the widespread use of antibi-otics and the emergence of multiple drug-resistant bacteria pose great challenges to clinical antimicrobial ther-apy and global health safety.The exploration and development of new antibacterial drugs has attracted world-wide attention.In this paper,the recent research on the antibacterial effect of chlorogenic acid and the mecha-nism of immune system regulation are reviewed,aiming to provide a new strategy for the development of new antibacterial drugs and the selection of clinical infectious diseases drugs.

Hv-S/TPK gene, a resistance related gene to powdery mildew, was cloned by using genechip, and its expression was upregulated after the inoculation of Blumeria graminis to Haynaldia villosa. Using the specific primers of Hv-S/TPK to screen a genomic TAC (Transformation-competent artificial chromosome) library of translocation line 6VS/6AL, a positive TAC was screened. A 5-kb fragment containing Hv-S/TPK was subcloned and identified. This 5160-bp fragment (GenBank Accession No. EU153366) was determined by specific primer walking. The analysis of Hv-S/TPK genomic sequence showed three introns and four extrons between start code and stop code. In the promoter region of Hv-S/TPK, there were W-box and OCS-like elements which were the elements related to disease resistance. In this study, the positive TAC clone was used to as probe in situ hybridized to mitotic metaphase chromosomes of translocation line. The result of fluorescence in situ hybridization (FISH) indicated that the TAC clone containing Hv-S/TPK was from Haynaldia villosa chromosome.
Base Sequence ; Chromosomes, Artificial ; Cloning, Molecular ; Genomic Library ; Molecular Sequence Data ; Plant Diseases ; genetics ; Poaceae ; genetics ; Protein-Serine-Threonine Kinases ; genetics ; Serine ; genetics ; Translocation, Genetic ; Triticum ; genetics ; virology