Objective To identify the sequence variation of human leukocyte antigen(HLA)novel allele A11:193 and to simulate the three-dimensional structure of the protein molecule.Methods A sample with abnormal allele results was found by PCR-SBT sequencing and identified by single allele specific sequencing.The 3D structure of the encoded protein was analyzed by Swiss-Model.Results Compared with HLA-A*11:01:01,which has the highest homology,exon 4 nt 662 of this sample has a base substitution of A→G,and its corresponding codon 197 is changed from CAT to CGT,which is changed from histidine(His)to arginine(Arg).Conclusion A new allele of HLA-A was confirmed.The allele sequence was named HLA-A11:193 by the WHO HLA Factor Nomenclature Committee and the three-dimensional structure of the protein molecule encoded by HLA-A11:193 was simulated.There was no significant difference in the three-dimensional structure of the encoded protein between it and HLA-A*11:01:01.

Objective To identify the sequence variation of human leukocyte antigen(HLA)novel allele A11:193 and to simulate the three-dimensional structure of the protein molecule.Methods A sample with abnormal allele results was found by PCR-SBT sequencing and identified by single allele specific sequencing.The 3D structure of the encoded protein was analyzed by Swiss-Model.Results Compared with HLA-A*11:01:01,which has the highest homology,exon 4 nt 662 of this sample has a base substitution of A→G,and its corresponding codon 197 is changed from CAT to CGT,which is changed from histidine(His)to arginine(Arg).Conclusion A new allele of HLA-A was confirmed.The allele sequence was named HLA-A11:193 by the WHO HLA Factor Nomenclature Committee and the three-dimensional structure of the protein molecule encoded by HLA-A11:193 was simulated.There was no significant difference in the three-dimensional structure of the encoded protein between it and HLA-A*11:01:01.

Objective:To explore the current status of gastrointestinal complications in patients after cardiac surgery and analyze its influencing factors.Methods:This study was cross-sectional study. From October 2019 to April 2020, convenience sampling was used to select 152 patients who underwent cardiac surgery in the Department of Adult Cardiac Surgery of a ClassⅢ Grade A hospital in Henan Province as the research object. We collected and recorded whether patients had gastrointestinal complications and its related factors after surgery. Binary Logistics multivariate analysis was used to explore the influencing factors of gastrointestinal complications.Results:The incidence of gastrointestinal complications in patients after cardiac surgery was 9.9% (15/152) . Logistic regression analysis showed that the influencing factor of gastrointestinal complications included the patient's preoperative left ventricular ejection fraction ( OR=0.849) , cardiopulmonary bypass time ( OR=0.974) , aortic block time ( OR=1.056) , intraoperative blood loss ( OR=1.007) , intraoperative blood transfusion ( OR=0.996) , time in Intensive Care Unit ( OR=1.463) , type of surgery [coronary artery bypass grafting (CABG) OR=0.199, valve replacement/valvuloplasty OR=0.105, CABG+ valve replacement/valvuloplasty OR=0.006] ( P<0.05) . Conclusions:There are many factors influencing gastrointestinal complications after cardiac surgery. Medical and nursing staff should strengthen the assessment of risk factors and perform predictive care in order to minimize the occurrence of complications and improve the prognosis of patients.