The evaluation of the bioavailability of pollutants in soil is crucial to accurately assess the pollution risk, and whole-cell biosensor is one of the important tools for such evaluation. This study aimed to develop a novel whole-cell biosensor for the detection of methyl parathion in soil using. First, a whole-cell biosensor was constructed by the screened methyl parathion hydrolase mpd gene, the existing specific induction element pobR, and the pUC19 plasmid skeleton. Then, the detection method of methyl parathion in soil extracts was established using 96-well microtiter plate as carrier and five whole-cell biosensors as indicator. The method was applied in the detection of methyl parathion in tested and field soil extracts. Taking E. coli DH5α/pMP-AmilCP with the best detection performance as an example, this biosensor had a detection limit of 6.21-6.66 µg/L and a linear range of 10-10 000 µg/L for methyl parathion in four soil extracts. E. coli DH5α/pMP-RFP and E. coli DH5α/pMP-AmilCP methods have good detection performance for the analysis of methyl parathion in soil extract samples. This biosensor method can help to quickly assess the bioavailability of methyl parathion in soil, and thus help to understand the risk of soil pollution caused by organophosphorus pesticide methyl parathion.
Methyl Parathion/analysis* ; Pesticides/analysis* ; Organophosphorus Compounds ; Escherichia coli/genetics* ; Soil ; Farms ; Biosensing Techniques

Chromatography, Gas ; methods ; Dimethoate ; blood ; Humans ; Insecticides ; blood ; Methyl Parathion ; blood

Animals ; Chromatography, Thin Layer ; Dichlorvos ; blood ; Dimethoate ; blood ; Insecticides ; blood ; Methyl Parathion ; blood ; Mice ; Organothiophosphorus Compounds ; blood

Poisoned the rats by pesticide wofatox with dose 10mg/kg of their body weight. After 60 minutes, cut off two third of liver by method of Higgins and Anderson. The changes in morphological structures and mitotic activity of liver rats were as follows: Lesions in the liver were markedly manifested, but were not prolong. The mitotic activity of hematocytes decreased. The regenenation of liver weakly manifested
Methyl Parathion ; Rats ; Liver

Study on the changes of ultra and micro-structure of the liver of white rats, which was effected by Wafatox with longed-low dose. Rats with the beginning body mass of 120-150g were everyday oral administrated by oil containing 0.1% of Wafatox with the dose of 0.5mg/kg of their body mass (similarly with 1/70 dose of LD50 for white rats). They were killed by stages of 10, 20, 30, 40 and 50 days from the first oral administration. The results: in clinical, the rat’s intoxicated symptoms were not clear, but the changes in the liver were clearly shown. In the liver, there were some points of necrozism, hepatocytes strongly regressed at the level of ultra and micro-structure. The portals areas and the liver’s tissues were infiltrated by cells like lymphocytes. There were happened stretching veins, stagnating blood and particularly, getting thicker and thicker in the walls of veins of central lobules of the rats’ liver at every stage. Up to the 50th day of intoxication by Wafatox, the quantity of two-nucleus hepatocytes was decreased and mitotic activity of hepatocytes was not increased
Methyl Parathion ; Rats ; Liver

Adult ; Antidotes ; administration & dosage ; therapeutic use ; Female ; Humans ; Insecticides ; poisoning ; Parathion ; poisoning ; Poisoning ; drug therapy ; Pralidoxime Compounds ; administration & dosage ; therapeutic use ; Treatment Outcome

AIMTo explore the effect of exposure to commercial Parathion (Pc) on the reproductive parameters (sperm and cocoon production and genotoxicity on male germ cells), the survival, the body weight and the gross anatomical changes in Eisenia foetida.

METHODSThree doses of Pc (1478, 739 and 444 mg/kg of soil) and three time intervals of exposure (5, 15 and 30 days) were used.

RESULTSAll treated animals were affected. An acute genotoxic effect, revealed by DNA fragmentation (comet assay), was seen by 5 days. Alterations in reproductive parameters were conspicuous in regard to the number of sperm, cocoons and worms born, and the histological observation of the gonads and seminal receptacles. In addition, the body weight and survival rate were decreased. Neuromuscular function was also affected.

CONCLUSIONEarthworms are suitable bioindicators of chemical contamination of the soil, their advantage being their easy and economical handling.

Animals ; Behavior, Animal ; drug effects ; Insecticides ; toxicity ; Male ; Oligochaeta ; drug effects ; Parathion ; toxicity ; Reproduction ; drug effects ; Soil Pollutants ; toxicity ; Survival Rate

The aquatic quality of the Naktong river after two of three months in June, 1991 with phenol spillage from a electrical factory in Kumi was investigated. The samples were collected at six sites of the Naktong river basin and Kachang and Kongsan lakes. Phenol was not detected from all water samples. Turbidity was very much increased to the down stream in the Naktong river. The BOD and COD values exceeded the 2nd grade(3 mg/l) of the Korean standard quality of Environmental Water Act at the all sampling sites of the Naktong river. Especially, the value of COD at Kaejin (12.5 mg/l) was poorly classified as to the 5th grade of water class for the environmental quality standards. Organophosphorous pesticides such as parathion, malathion, fenitrothion and diazinon were investigated but not detected. Diazinon was only detected at the Ilson bridge(1.42 ppb), Okkye stream(6.95 ppb), Waekwan bridge(0.32 ppb), Gangjung reservior(0.13 ppb), Kaejin(0.05 ppb). Of the carbamates such as carbanyl, isoprocarb and cabofuran, the carbofuran was detected all sites except tap water, and Kachang and Kongsan lakes. The content of heavy metals such ans Cd, Pb, Zn, Fe, Mn, Hg were not exceeding for drinking water standards at the all sampling region, but only mecury was detected from Okkye stream(0.018ppb) and Kaejin(0.09ppb). In the regions of Kachang and Kongsan lakes, the content of heavy metals were lower than that of reservoir of Naktong river.
Carbamates ; Carbofuran ; Diazinon ; Drinking Water ; Fenitrothion ; Gyeongsangbuk-do* ; Korea* ; Lakes ; Malathion ; Metals, Heavy ; Parathion ; Pesticides ; Phenol* ; Rivers* ; Water ; Water Quality

This survey was conducted to find out demage done to the farmers by ;Agrochemicals in a rural area of Korea from January to October, 1980. Choon Sung Gun, Kang Won Province was the survey area and the 412 males were surveyed among all those that have sprayed agrochemicals. during 10 Months in 1980. Obtained results and findings from this survey are summarized as follows; 1. The total spray days of 413 males were 3,114 days and avarage spray days per person were 7.54 days. Also avarage spray hours per person were 4.7 hours. 2. The incidence rate per 100 persons of self-recognized skin manifestation was 12.6 persons and incidence rate per spray day was 2.7 percent. The incidence rate per 1Q0 persons of self-recognized intoxication was 23.0 persons and incidence rate per spray day was 3.6 percent. 3. In cases where mask was not used, when it was syrayed in hot weather, when stronger solution was used, the results were higher percentage in self-recognized intoxication (P<0.01). 4. The symptoms of self-recognized intoxication were headache (55.8%), dizziness (46.9%),. nausea (17.7%), fatigue (17.0%), and vomiting (17.0;%). 5. Number of intoxication per 100 used standardized unit by agrochemicals was parathion (93.8 spells), sumithion (91.8 spells) and folithion (66.7 spells). 6. Treatment was done by health facility utilization (27 cases), visits to drug store (13 cases) and visits to health center (7 cases).
Agrochemicals* ; Dizziness ; Fatigue ; Fenitrothion ; Gangwon-do ; Headache ; Health Facilities ; Humans ; Incidence ; Korea* ; Male ; Masks ; Nausea ; Parathion ; Skin Manifestations ; Vomiting ; Weather

The Authors observed 16 cases of Parathion intoxication from 1972 to 1974 in LC.U. of Busan National University Hospital. The results were as follows; 1. Parathion solution (20%) was taken for the purpose of suicide in all 16 cases. 2. Male-to-female ratio was 1. 28: 1. Age distribution ranged from 18 to 31, and the highest age group was 2 nd decade (ll cases), next 3 rd (3 cases), and 1st decade (2 cases) in order. 3. Culture of bronchial secretion was done in 6 cases and Pseudomonas Aeruginosa were detected in 5 cases. 4. Adequate control of respiratory problems and drug therapies for this intoxication resulted.
Age Distribution ; Busan ; Drug Therapy ; Humans ; Parathion* ; Pseudomonas aeruginosa ; Suicide