Objective: To investigate the independent prognostic factors affecting disease-free survival(DFS) after radical surgery in Ⅱ and Ⅲ adenocarcinoma of gastroesophageal junction(AEG) patients, and to construct a nomogram prediction model. Methods : A retrospective analysis was conducted on a cohort comprising 326 patients who were diagnosed with AEG. Chi-square test was used for categorical variable analysis. The survival analysis was drawn by Kaplan-Meier method and tested by log-rank method. Univariate and multivariate Cox regression analyses identified independent prognostic factors, and based on these factors, a nomogram was constructed to predict 3-year and 5-year DFS in AEG patients. The calibration curves evaluated the performance of nomogram. Results: Among 326 AEG patients, 281 were in the young group(<70 years old) and 45 were in the old group(≥70 years old). Kaplan-Meier curve showed that patients with advanced age, TNM stage Ⅰ-Ⅱ and no history of hepatitis B had longer DFS. Univariate and multivariate analysis showed that age, TNM stage and history of hepatitis B were independent prognostic factors for DFS. The 3-year and 5-year correction curves showed that the prediction efficiency of nomogram was good. The decision curve analysis showed that the model had a good clinical net benefit. Conclusion Age, TNM stage and history of hepatitis B are independent prognostic factors for DFS in AEG patients. The nomogram constructed and validated can be used to predict DFS in AEG patients.

Objective To investigate the function and clinical significance of nuclear matrix binding region binding protein 1(SATB1)and epidermal growth factor receptor(EGFR)in gastric cancer.Methods The expression of SATB1 and EGFR was detected by immunohistochemistry in 60 cases of paraffin-embedded gastric cancer and 39 cases of gastric mucosal inflammation.Small interfering RNA(siRNA)was transfected into gastric cancer cells.The expression of SATB1 and EGFR after transfection was detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction(RT-qPCR)and Western blot.The cell migration and invasion changes of MGC803 after transfection were detected by cell scratch test and Transwell test.The prognosis of gastric cancer pa-tients with different SATB1 and EGFR expression was analyzed by KM survival analysis.Results The expression of SATB1 and EGFR in gastric cancer tissues was higher than that in gastric mucosa inflammation tissues,and the difference was statistically significant(P＜0.05).The expression of SATB1 was correlated with lymph node metas-tasis and distant metastasis of gastric cancer,and the difference was statistically significant(P＜0.05).EGFR ex-pression was also associated with distant metastasis,and the difference was statistically significant(P＜0.05).There was a positive correlation between the expression of SATB1 and EGFR in gastric cancer tissues(r=0.49,P＜0.05).RT-qPCR and Western blot showed that siRNA could effectively knock down the expression of SATB1 and EGFR in gastric cancer cells.The results of cell scratch and Transwell experiments showed that the migration and invasion of gastric cancer cells were inhibited by knocking down SATB1 and EGFR.The results of KM survival curve showed that gastric cancer patients with low expression of SATB1 had a better prognosis,while those with low expression of EGFR had a better prognosis.Conclusion The expression levels of SATB1 and EGFR are closely re-lated to lymph node metastasis and distant metastasis of gastric cancer,and decreasing the expression levels of SATB1 and EGFR can inhibit the migration and invasion of gastric cancer.