Objective:To explore the correlation between serum glucose regulated protein 78 (GRP78) and prohibitin1 1 (PHB1) with poor prognosis in emergency sepsis patients.Methods:A prospective study was conducted to select 99 patients with sepsis admitted to the emergency department of the Zibo 148 Hospital from January 2021 to January 2024 as the study subjects. After admission, serum GRP78 and PHB1 levels were tested, and the survival status of patients within 28 d of admission was statistically analyzed. In addition, 99 non-infected people who came to the Zibo 148 Hospital for physical examination during the same period were taken as the control group. The serum levels of GRP78 and PHB1 between patients with sepsis and the control group were compared. Baseline data and serological indicators of sepsis patients with different prognoses were compared. Correlation analysis, Logistic regression analysis and receiver operating characteristic curve (ROC) were used to explore the relationship between serum GRP78, PHB1 and poor prognosis in patients with emergency sepsis.Results:The serum levels of GRP78 and PHB1 in sepsis patients were higher than those in the control group: (125.08 ± 27.92) μg/L vs. (97.73 ± 15.62) μg/L, (186.65 ± 26.85) ng/L vs. (147.89 ± 24.63) ng/L, there was statistical difference ( P<0.05). Among the 99 patients with sepsis, 25 patients (25.25%) died within 28 d of admission. The deceased patients were included in the poor prognosis group, while the remaining 74 patients (74.75%) were included in the good prognosis group. The age of the poor prognosis group was older than that of the good prognosis group: (66.52 ± 4.72) years vs. (62.24 ± 3.67) years, the proportion of diabetes in the good prognosis group was higher: 72.00% (18/25)vs. 25.68%(19/74), the infectious shock group was higher than that of the good prognosis group: 60.00% (15/25) vs. 16.22%(12/74), and the serum GRP78 and PHB1 levels were higher than those in the good prognosis group: (149.50 ± 31.53) μg/L vs. (116.82 ± 21.13) μg/L, (209.91 ± 19.06) ng/L vs. (178.79 ± 24.51) ng/L, there were statistical differences ( P<0.05). The correlation analysis showed that age, diabetes, disease severity, serum GRP78, PHB1 were associated with poor prognosis of emergency sepsis patients (correlation coefficient was 0.428, 0.416, 0.427, 0.511, 0.506, P<0.05). Logistic regression analysis showed that serum GRP78, PHB1, age, diabetes and severity could affect the poor prognosis of emergency sepsis patients ( P<0.05). ROC curve was drawn, and the results indicated that the area under the curve value of serum GRP78 and PHB1 combined to predict poor prognosis in patients with emergency sepsis was 0.889 (95% CI 0.823 - 0.955), the sensitivity was 96.00%, and the specificity was 66.20%, which was higher than any single index. Conclusions:The occurrence of poor prognosis in patients with emergency sepsis is related to the increased expression of serum GRP78 and PHB1.Clinically, the risk of poor prognosis in patients with sepsis can be evaluated in advance by the levels of serum GRP78 and PHB1 detected at admission.

Electrical impedance tomography (EIT) is a new non-invasive functional imaging technology, which has the advantages of non-invasion, non-radiation, low cost, fast response, portability and visualization. In recent years, more and more studies have shown that EIT has great potential in the detection of lung diseases and has been applied to early diagnosis and treatment of some diseases. This paper introduced the basic principle of EIT, discussed the research and clinical application of EIT in the detection of acute respiratory distress syndrome, chronic obstructive pulmonary disease, pneumothorax and pulmonary embolism, and focused on the summary and introduction of indicators and functional images of EIT related to the detection of lung diseases. This review will help medical workers understand and use EIT, and promote the further development of EIT in lung diseases as well as other fields.
Humans ; Electric Impedance ; Tomography/methods* ; Lung Diseases/diagnosis* ; Pulmonary Disease, Chronic Obstructive/diagnosis* ; Pulmonary Embolism/diagnosis* ; Respiratory Distress Syndrome/diagnosis*

Objective:To explore the correlation between serum glucose regulated protein 78 (GRP78) and prohibitin1 1 (PHB1) with poor prognosis in emergency sepsis patients.Methods:A prospective study was conducted to select 99 patients with sepsis admitted to the emergency department of the Zibo 148 Hospital from January 2021 to January 2024 as the study subjects. After admission, serum GRP78 and PHB1 levels were tested, and the survival status of patients within 28 d of admission was statistically analyzed. In addition, 99 non-infected people who came to the Zibo 148 Hospital for physical examination during the same period were taken as the control group. The serum levels of GRP78 and PHB1 between patients with sepsis and the control group were compared. Baseline data and serological indicators of sepsis patients with different prognoses were compared. Correlation analysis, Logistic regression analysis and receiver operating characteristic curve (ROC) were used to explore the relationship between serum GRP78, PHB1 and poor prognosis in patients with emergency sepsis.Results:The serum levels of GRP78 and PHB1 in sepsis patients were higher than those in the control group: (125.08 ± 27.92) μg/L vs. (97.73 ± 15.62) μg/L, (186.65 ± 26.85) ng/L vs. (147.89 ± 24.63) ng/L, there was statistical difference ( P<0.05). Among the 99 patients with sepsis, 25 patients (25.25%) died within 28 d of admission. The deceased patients were included in the poor prognosis group, while the remaining 74 patients (74.75%) were included in the good prognosis group. The age of the poor prognosis group was older than that of the good prognosis group: (66.52 ± 4.72) years vs. (62.24 ± 3.67) years, the proportion of diabetes in the good prognosis group was higher: 72.00% (18/25)vs. 25.68%(19/74), the infectious shock group was higher than that of the good prognosis group: 60.00% (15/25) vs. 16.22%(12/74), and the serum GRP78 and PHB1 levels were higher than those in the good prognosis group: (149.50 ± 31.53) μg/L vs. (116.82 ± 21.13) μg/L, (209.91 ± 19.06) ng/L vs. (178.79 ± 24.51) ng/L, there were statistical differences ( P<0.05). The correlation analysis showed that age, diabetes, disease severity, serum GRP78, PHB1 were associated with poor prognosis of emergency sepsis patients (correlation coefficient was 0.428, 0.416, 0.427, 0.511, 0.506, P<0.05). Logistic regression analysis showed that serum GRP78, PHB1, age, diabetes and severity could affect the poor prognosis of emergency sepsis patients ( P<0.05). ROC curve was drawn, and the results indicated that the area under the curve value of serum GRP78 and PHB1 combined to predict poor prognosis in patients with emergency sepsis was 0.889 (95% CI 0.823 - 0.955), the sensitivity was 96.00%, and the specificity was 66.20%, which was higher than any single index. Conclusions:The occurrence of poor prognosis in patients with emergency sepsis is related to the increased expression of serum GRP78 and PHB1.Clinically, the risk of poor prognosis in patients with sepsis can be evaluated in advance by the levels of serum GRP78 and PHB1 detected at admission.