Introduction: The National Library of Mongolia was established in 1921 based largely on the 8th Khalkha Jebtsundamba Library. In studying the tradition of collecting and preserving books in Mongolia, the subject can be divided into three categories:
1. Family collection
2. Palace/ Royal collections
3. Monastery collections.
From the collection mentioned above, we selected a work belonging to the royal collections, specifically one transferred from the VIII Khalkha Jebtsundamba’s Library to the National Library. This book, “The drug actions that are useful for any kind of ulcer, swelling, and injuries,” is a rare and valuable item of medical cultural heritage, existing in a single known copy. Therefore, this work was selected for incorporation into the research cycle.
To identify the formulas, forms of medicines, methods of use, actions, and to abstain from diet methods in the book “The drug actions that are useful for any kind of ulcer, swelling, and injuries.” Methods: We employed methods such as the study of original texts and sources, analytic- synthetic method. Conclusion The book includes methods for using 27 various medicines to treat 26 types of diseases related to the lesions. Some medicines can be used externally in addition to oral administration, and 90% of all medicines are in the form of powders and granules for external application.

Introduction: Inula helenium L. is a medicinal plant widely used in traditional Mongolian and Tibetan medicine. Its roots and rhizomes contain active compounds, including sesquiterpene lactones (alantolactone and isoalantolactone), inulin, flavonoids, and essential oils, with anti-inflammatory, antimicrobial, and immunomodulatory properties. This study evaluates the quality of Inula helenium L. roots and quantifies their key active compounds in accordance with pharmacopoeial standards. Methods: The roots of Inula helenium L. cultivated in the Medicinal Plant Botanical Garden of Monos Group were used as raw material. Physicochemical parameters, including moisture, total ash, acid-insoluble ash, and extractive values, were determined according to pharmacopoeial methods. Qualitative analysis was carried out using color reactions for polysaccharides and thin-layer chromatography for the identification of sesquiterpene lactones. Quantitative analysis was performed using spectrophotometry for total polysaccharides and high-performance liquid chromatography (HPLC) for the determination of alantolactone and isoalantolactone. Conclusion The physicochemical parameters of Inula helenium L. root were standardized, with total inulin content determined as 47.13±0.45%, alantolactone as 1.41±0.02%, and isoalantolactone as 1.33±0.02%. Analytical methods for inulin, alantolactone, and isoalantolactone were validated according to ICH Q2(R2) guidelines, confirming the possibility of updating the MNS standard or developing a manufacturer’s pharmacopoeial monograph based on these validated methods.

Background: Elite athletes regularly participate in national and international sporting competitions while simultaneously engaging in high-intensity and intensified training programs. Therefore, assessing changes in blood biochemical parameters is of considerable importance for evaluating athletes’ physiological adaptation to training loads. In particular, determining the activity of lactate dehydrogenase enzyme in athletes’ blood plasma provides a scientific basis for improving individual performance, evaluating sports performance, and enhancing the effectiveness of future training programs. Aim: To examine plasma lactate dehydrogenase enzyme activity in athletes of the Mongolian National Team. Materials and Methods: A total of 51 athletes, including 26 men and 25 women from 6 types of sports of the Mongolian National Team included in the study. Anaerobic capacity was assessed using a Monark 894E Ergomedic Peak Bike, designed to apply exercise load. Blood serum lactate level and lactate dehydrogenase enzyme activity were determined using a Biobase BK-280 fully automated biochemical analyzer. Heart rate, peripheral blood oxygen levels, and oxygen saturation were measured using a pulse oximeter. Result: The average age of the participating was 24.04±4.15 years, with average height of 168±8.78 cm and average weight of 71.01±7.69 kg. The average BMI was 24.82±4.12 kg/m². Pre-exercise lactate dehydrogenase enzyme activity averaged 177.1±28.96 U/L, while post-exercise lactate dehydrogenase activity averaged 223.5±51.56 U/L. The average heart rate before exercise was 66.04±8.9 bpm, while post-exercise heart rate was 123.6±16.06 bpm. The average VO2 max was 95.18±2.48. Conclusion The activity of the enzyme lactate dehydrogenase before and after exercise showed significant differences in the age groups 20-29 (p<0.01). When comparing lactate dehydrogenase enzyme activity before and after exercise by sport, statistically significant increases were observed in freestyle wrestling and judo athletes (p<0.01).

Background: Academic burnout negatively affects academic performance. Excessive academic workload may lead students to disengagement from learning, reduced motivation, mental fatigue, and chronic stress. Aim: To examine academic burnout and its influencing factors among nursing students. Materials and Methods: This cross-sectional study was conducted among undergraduate nursing students’ years 1–4 at the School of Nursing, MNUMS. From a total population of 671 students, 250 were selected using simple random sampling. Data were collected between December 2024 and February 2025 using the Maslach Burnout Inventory Scale (MBI). Result: Participants ranged in age from 17 to 52 years; 242 (96.8%) were aged 22 years or younger, with a mean age of 20.01 years. The overall academic burnout score was 74, indicating a moderate level of burnout. Age, marital status, and employment status were not significantly associated with overall burnout. However, personal burnout (r=.95, p<.000), study-related burnout and peer-related burnout (r = .98, p<.000), and teacher-related burnout (r=.94, p<.000) showed that strong positive correlations and were statistically significant. Conclusion Personal factors, study-related factors, peer-related factors, and teacher-related factors contributing to burnout were each at a moderate level.

Background: Type I hypersensitivity, commonly known as an allergic reaction, is characterised by an IgE-mediated immune response triggered when foreign proteins enter the body. During allergic reactions, cytokines such as IL-4, IL-5, IL-9, and IL-13, secreted by T-helper 2 (Th2) cells, play a crucial role in activating immune effector cells like eosinophils and mast cells, which contribute significantly to airway inflammation and obstruction. Conversely, interferon-gamma (IFN-γ), produced by Th1 cells, suppresses Th2-mediated responses and plays an essential role in regulating allergic inflammation. Therefore, determining the balance of cytokines produced by Th1 and Th2 cells is crucial for understanding the mechanisms underlying allergic responses. Aim: We aimed to induce an allergic response in experimental animals using ovalbumin and to determine the levels of IL-4 and IFN-γ cytokines using an in-house enzyme-linked immunosorbent assay (ELISA). Materials and Methods: A total of 20 female C57BL/6 mice aged 6–8 weeks were selected for the experiment and randomly divided into two groups: control and experimental groups. The concentrations of IL-4 and IFN-γ cytokines in mouse serum were determined using an in-house enzyme-linked immunosorbent assay (ELISA). Result: Compared to the control group, the levels of IL-4 and IFN-γ cytokines in the serum of allergic model-induced C57BL/6 mice were determined to be 513.7 pg/mL and 193.2 pg/mL, respectively. A statistically significant difference was observed in IL-4 cytokine levels between the two groups (p < 0.001). Conclusion In the allergic model group, serum levels of IL-4 cytokine were elevated, while IFN-γ cytokine levels were decreased, suggesting a predominant Th2-type immune response.

Background: Biotinidase deficiency is an autosomal recessive disorder of biotin metabolism that can be effectively managed when detected early through newborn screening. Biotinidase activity is influenced by neonatal and preanalytical factors, which may affect the interpretation of screening results. Following the introduction of biotinidase deficiency screening in Mongolia in 2025–2026, population-specific data are needed to support accurate screening interpretation. Aim: To determine the distribution and percentile values of biotinidase activity in Mongolian newborns and evaluate associated neonatal and preanalytical factors. Materials and Methods: This analytical cross-sectional study included 6,764 newborns from 12 healthcare facilities in Mongolia between August 2025 and May 2026. After excluding initial screen-positive samples and those with transport times exceeding 30 days, 6,258 newborns were included in the primary analysis. Biotinidase activity was measured in dried blood spots using the Neonatal Biotinidase 3018-0010 kit and VICTOR2D analyzer. Percentile curves by gestational age and birth weight were smoothed using LOWESS. Group differences and associations were assessed using Welch ANOVA and Pearson correlation, respectively. Result: Among 6,258 newborns, mean biotinidase activity was 133.3±32.6 U, with a median of 130.2 U and a P2.5-P97.5 interval of 78.4-206.6 U. Median activity tended to increase with gestational age and birth weight, while the smoothed percentile curves remained above the 58.5 U screening cutoff. Mean activity increased from 124.4±34.1 U in samples collected before 24 hours to 149.9±44.5 U in those collected after 96 hours (p<0.001). Activity showed weak positive correlations with gestational age (r=0.042, p=0.001), birth weight (r=0.114, p<0.001), and body length (r=0.102, p<0.001), but negative correlations with time from sample collection to laboratory receipt (r=−0.327, p<0.001) and from laboratory receipt to testing (r=−0.154, p<0.001). Conclusion Population-specific percentile values for biotinidase activity were established in a large sample of Mongolian newborns. The findings provide baseline data for interpreting biotinidase activity and highlight the importance of standardized sample collection, transport, and testing.

Background: Adhesion small bowel obstruction (ASBO) is a common complication following abdominal surgery and can significantly impair postoperative recovery. This condition necessitates the exploration of more effective therapeutic approaches. Aim: The aim of this study was to evaluate the clinical efficacy of Traditional Mongolian Medicine (TMM) combined with acupuncture in the management of postoperative ileus secondary to adhesion-related small bowel obstruction. Case Presentation: We report the case of a 47-year-old female patient who developed postoperative ileus. The therapeutic intervention consisted of a combined regimen of traditional herbal pharmacotherapy and electroacupuncture. The clinical outcomes were assessed based on the resolution of symptoms and the restoration of gastrointestinal function. Result: Following the administration of the integrated treatment, the patient exhibited a marked reduction in abdominal pain. Objective clinical improvement was noted through the successful restoration of intestinal peristalsis and the normalization of flatus and defecation. Conclusion The findings of this case study suggest that Traditional Mongolian Medicine, particularly when integrated with electroacupuncture, may be an effective and viable modality for promoting the recovery of gastrointestinal motility in patients with adhesion-related small bowel obstruction.

Background: Sarcopenia is a multifactorial geriatric syndrome characterized by age-related loss of muscle mass, muscle strength, and physical performance. In recent years, neurotrophic and neuromuscular biomarkers have been investigated as potential diagnostic indicators of sarcopenia; however, evidence regarding their sex-specific differences remains limited. Aim: To evaluate the sex-specific associations of serum BDNF, GDNF, and CAF22 levels with sarcopenia and muscle-related parameters among older adults. Materials and Methods: This analytical cross-sectional study included adults aged 60 years and older. Sarcopenia was defined according to the Asian Working Group for Sarcopenia (AWGS 2019) criteria. Serum BDNF, GDNF, and CAF22 levels were measured using ELISA. Group differences were analyzed using independent t-tests, correlations were assessed using Pearson correlation analysis, and diagnostic performance for sarcopenia was evaluated using receiver operating characteristic (ROC) analysis. Multivariable logistic regression analysis was also performed. Results: No statistically significant differences were observed in serum BDNF, GDNF, and CAF22 levels between sarcopenic and non-sarcopenic participants in either the male or female groups (all p>0.05). ROC analysis demonstrated AUC values ranging from 0.540 to 0.542 in males and from 0.508 to 0.530 in females for BDNF, GDNF, and CAF22. Multivariable logistic regression analysis revealed that age (OR=1.12, 95% CI: 1.04–1.21, p=0.003) and lower fat-free mass index (OR=0.78, 95% CI: 0.66–0.91, p=0.001) were significantly associated with sarcopenia, whereas BDNF, GDNF, and CAF22 showed no independent association with sarcopenia. Conclusions 1. No statistically significant sex-specific differences were observed in serum BDNF, GDNF, and CAF22 levels between sarcopenic and non-sarcopenic older adults (all p>0.05). 2. CAF22 levels were weakly negatively correlated with muscle mass and fat-free mass index in males (p<0.05), whereas BDNF levels showed weak positive correlations with handgrip strength and fat-free mass index in females (p<0.05). CAF22 levels also demonstrated a weak positive correlation with age (p<0.05). 3. ROC and multivariable logistic regression analyses indicated limited diagnostic utility of BDNF, GDNF, and CAF22 for sarcopenia (AUC=0.508–0.542), and none of these biomarkers showed an independent association with sarcopenia (all p>0.05). In contrast, older age (OR=1.12, p=0.003) and lower fat-free mass index (OR=0.78, p=0.001) were significantly associated with sarcopenia.

Introduction: The World Health Organization (WHO) reports that noise pollution leads to a variety of short-term and long-term health issues, including sleep disorders, cardiovascular diseases, impaired concentration, decreased performance at work and school, and hearing difficulties. It has been established that for every 10 dB increase in environmental noise, the incidence of heart attacks among individuals aged 65 and older increases by 3.9% compared to those living in quieter environments (Seidler, et al., 2016). Furthermore, it has been found that noise pollution exceeding 65 dB is directly related to hypertension among residents living along high-traffic roads. Objective: To assessing traffic load and noise pollution on the roads of Ulaanbaatar Materials and Methods: The study was a cross-sectional survey conducted to determine noise pollution at 15 locations along roads such as Sun Road, Peace Avenue, Ard-Ayush Street, and Ikh Toiruu. Measurements were taken during morning (7-9 AM), afternoon (12-2 PM), evening (5-7 PM), and night (11 PM-1 AM) using a Noise Meter, Sound Level Meter, and portable equipment. Measurements were conducted at one point 100-200 meters from the main road, with three repetitions to assess hearing levels. Statistical analysis was performed using SPSS-24 software, and the results were compared to the Air Quality Technical General Requirements MNS 4585:2016 standard (During the day (07:00-22:00), 60 dB(A), and at night (22:00 07:00), 45 dB(A)). Results: The measurement results revealed that the maximum noise value in the morning (7-9 AM) was 83 dB(A), the minimum noise value was 51 dB(A), and the average of the maximum noise values was 74.1±4.7 dB(A). The maximum noise value during the day was 97 dB(A), the minimum noise value was 47 dB(A), and the average of the maximum noise value was 74.4±6.9 dB(A). In the evening, the maximum noise value was 88 dB(A), the minimum noise value was 47 dB(A), and the average of the maximum noise value was 74.4±6.6 dB(A). For the night measurements, the maximum noise value was 86 dB(A), the minimum noise value was 49 dB(A), and the average of the maximum noise value was 69.4±7.7 dB(A).
When calculating the average noise pollution on weekdays and weekends, the average of the max and min values during the morning, evening, and night on weekdays was 0.59-4.33 dB(A) higher than that of the weekend. The average maximum value during the day on weekends was 2.79 dB(A) higher than that of weekdays. The number of vehicles participating in road traffic showed a strong positive correlation with environmental noise (Rxy=0.660, p=0.0001), and the number of times the horn was sounded also revealed strong positive correlation with environmental noise (Rxy=0.674, p=0.029). Conclusion The noise level along the main roads exceeds the permissible limits set by the "Air Quality. Technical General Requirements MNS 4585:2016" standard by 37 dB(A) during the day and by 41 dB(A) at night. The average maximum value during the day exceeds the standard by 14.6 dB(A). This indicates that Ulaanbaatar is experiencing noise pollution at levels that negatively affect the health of the urban population.

Background: Indoor air quality in schools is an important environmental health concern because children spend a substantial proportion of their time in classrooms and are particularly vulnerable to air pollutants. Objective: To assess common chemical, and biological pollutants in the indoor air of general education schools in Ulaanbaatar. Methods: A cross-sectional study was conducted from October 10, 2025, to March 1, 2026, in 16 purposively selected schools and 32 classrooms across six districts of Ulaanbaatar. PM2.5, PM10, CO₂, and microclimatic parameters were monitored continuously for seven days. Volatile organic compounds, formaldehyde, BTEX compounds, and radon were also measured. Airborne microorganisms were assessed using 160 air samples, and 160 surface swabs were collected. Results were compared with MNS 7106:2026, MNS 5484:2005, and MNS 6410:2018 standards. Results: The mean 24-hour concentrations of PM2.5 and PM10 were 25.2 and 36.1 µg/m³, respectively. PM2.5 exceeded the permissible level during winter and in schools located in apartment areas, whereas the mean PM10 concentration met the acceptable level. Total bacteria were detected in all air samples, with a mean concentration of 1,733.25 CFU/m³. Mould and fungi were detected in 56.3% of classrooms, and enteric bacteria were detected in 4.3% of surface swabs. Radon levels remained below the permissible limit, and pathogenic bacteria were not detected. Formaldehyde was detected in all samples, while total VOCs and toluene were detected in 81.3%; their mean concentrations remained within acceptable levels. In one classroom, the concentration of benzene was detected approximately 30 times higher the permissible level. Conclusion Although some indoor air-quality indicators met the relevant standards, PM2.5, total bacteria, mould and fungi, surface enteric bacteria, and benzene contamination require improvement and may pose health risks to children. Regular ventilation, improved cleaning and hygiene, proper maintenance of HEPA devices, and routine monitoring of particulate matter, mould, and VOCs are needed.