Background: In the treatment of plaque-induced gingivitis, in addition to mechanical plaque control, the use of chemical plaque control such as mouth rinses for a certain period has shown a positive effect on treatment outcomes and prognosis. Aim: To evaluate the efficacy of a probiotic mouthwash in the treatment of plaque-induced gingivitis. Materials and Methods: A randomized controlled clinical trial was designed for a period of 2 weeks on 45 systemically healthy subjects between 20 and 25 years having plaque-induced gingivitis. The study population was divided into three groups. Group 1-15 subjects were advised experimental (probiotic) mouthwash. Group 2-15 subjects were advised positive control (chlorhexidine) mouthwash and Group 3-15 subjects into a negative control group. Oral prophylaxis was done for all groups at baseline. After the proper oral hygiene instructions, groups 1 and 2 were instructed to rinse their mouth with 15 ml of their respective mouthwashes, for 1 min twice daily, 30 min after brushing. Clinical parameters such as Full mouth plaque score (FMPS), Full mouth bleeding score (FMBS) were assessed at baseline, 2 weeks respectively. Results: At baseline, there was no statistically significant difference between the groups in terms of FMBS and FMPS mean values (p=0.174, p=0.887). At day 14, the FMPS, FMBS mean values were significantly reduced by all treatment modalities ranking probiotic and chlorhexidine is greater than negative control group (p<0.001, p<0.001). Conclusion The probiotic mouthwash was effectively used as an adjunct to mechanical plaque control in the treatment of plaque-induced gingivitis.

Background: Meniere's disease is a condition caused by disturbances in the auditory and vestibular systems. It is characterized by symptoms such as dizziness, nausea, vomiting, and tinnitus. In the United States, the prevalence of Meniere's disease has been found to be 84 cases per 100,000 women and 56 cases per 100,000 men. Regionally, it is more common in less populated areas, and research has confirmed that the prevalence increases with higher household income. Aim: To evaluate the effectiveness of traditional medicine treatments and therapies for Meniere's disease. Results: The patient is a 43-year-old male, with symptoms of dizziness, vomiting, and tinnitus. Since 2023, he has sought care at the Central Hospital of Mongolian Medicine, where he received traditional medicine treatments including herbal therapy and other traditional therapies on three occasions. As a result, the frequency of dizziness has decreased and other clinical symptoms have improved. Conclusion Traditional medicine treatments have been shown to be effective in managing Meniere's disease by extending the interval between relapses, improving quality of life, and significantly reducing clinical symptoms.

Background: Rhododendron adamsii (Sagaan Dalya) is a medical plant widely distributed in the Altai region, Mongolia, and Russia. It is traditionally used for its calming, restorative, and immune-boosting properties. Inflammation is a complex immune response against pathogens such as bacteria, viruses, and fungi, involving macrophages, fibroblasts, mast cells, and neutrophils. These cells release inflammatory mediators such as nitric oxide (NO), TNF-α, and IL-1β. In collaboration with the Russian Foundation for Basic Research, a project was initiated to investigate the bioactive fractions of Rh. rosea (L.) and Rh. adamsii and their effects on the production of TLR4 signaling end products and associated protein activation. Previous studies within this project have shown that certain bioactive fractions exhibit anti-inflammatory activity. Specifically, fraction 7.11 suppressed NO production and inflammatory signaling molecules in LPS-stimulated RAW 264.7 macrophages, while fractions 7.46 and 7.52 influenced the phosphorylation of proteins such as ERK1/2, JNK, and IRF3. These findings suggest the need for further investigation into the effects of Rh. adamsii bioactive fractions on inflammatory signaling pathways. Aim: This study aims to evaluate the effects of bioactive fractions derived from Rhododendron adamsii on the production of TLR4 signaling end products in RAW264.7 macrophage cell cultures. Materials and Methods: The study was conducted using RAW264.7 macrophage cell cultures and bioactive fractions extracted from Rh. adamsii, dividing the experiments into three groups. After stabilization, RAW264.7 cells were stimulated with 100 ng/mL LPS. Based on previous studies, non-toxic concentrations of bioactive fractions (10 µg/mL) were applied. NO production was measured using the Griess assay, while TNF-α and IFN-β cytokine levels were evaluated using ELISA. Each experiment were repeated three times, and data were statistically analyzed using SPSS 25.0, applying One-way ANOVA and Independent Samples T-test. Results: The NO production in the positive control group was significantly higher compared to the negative control. In contrast, the experimental groups showed a statistically significant reduction in NO production, suggesting a potential inhibitory effect on TLR4 signaling in macrophages. However, fraction 7.48 reduced TNF-α levels while increasing IFN-β production, but these changes were not statistically significant. Similarly, fraction 7.55 slightly reduced TNF-α and IFN-β levels, but the effect was also statistically unsignificant. Conclusion The bioactive fractions 7.48 and 7.55 of Rh. Adamsii reduced nitric oxide (NO) production in LPS-stimulated macrophage cell line cultures, suggesting that they may inhibit TLR4 signaling. However, their lack of effect on TNF-α and IFN-β production indicates that they do not influence TLR4 signaling mediated by these cytokines. Instead, they may affect the final product output through other pathways or different stages of signal transduction.

Abstract Epilepsy, a neurological disorder characterized by recurrent seizures, affects millions of people worldwide. Significant advancements in both diagnostic and therapeutic approaches have greatly improved outcomes, particularly for individuals with drug-resistant epilepsy. Modern neurostimulation techniques such as Responsive Neurostimulation (RNS), Deep Brain Stimulation (DBS), and Vagus Nerve Stimulation (VNS) have demonstrated effectiveness in reducing seizure frequency in these patients. Additionally, emerging technologies like gene therapy and optogenetics are being explored to better understand the underlying mechanisms of epilepsy and hold promise as future treatment modalities.Traditional imaging techniques often fail to detect subtle epileptogenic zones. However, the introduction of ultra high-field 7T MRI scanners represents a major breakthrough. These advanced scanners use eight transmitters to generate higher-resolution images, reducing signal dropout and enabling the identification of previously undetected lesions. In one study involving 31 patients, 58% had their treatment plans modified based on 7T MRI findings, highlighting its potential to inform surgical decision-making. In terms of pharmacological treatment, newer medications such as sultiame, cannabidiol (CBD), and non-pharmacological approaches like the ketogenic diet have emerged as additional therapeutic options for drug-resistant epilepsy. These developments contribute to more effective, personalized management strategies for epilepsy. In summary, the landscape of epilepsy diagnosis and treatment is rapidly evolving. Innovations in imaging and therapeutic interventions are offering new hope for patients with drug-resistant epilepsy. Ongoing research and clinical trials remain essential to further refine these approaches and improve patient outcomes.

Introduction: In 2020, the prevalence of respiratory system diseases in the population of Gobi-Altai province is 1339.5 cases per 10,000 population, which is 4% (52) more than the average of the provinces and 20% (223.8) more than the national average. In 2021, the number of deaths due to respiratory system diseases was 2.9 per 10,000 population, increasing by 1.1 from 2020 (1.8). Pneumonia-related deaths account for 60.7% of all respiratory system-related deaths. As of 2022, there are 7,281 simple stoves, 248 low-pressure and steam boilers, and 18,207 automobiles in the Gobi-Altai province as sources of air pollution. 63.1% of the total coal is consumed by water heating boilers with a capacity of more than 101 kW, 20.5% by households, and 16.4% by small and medium enterprises. Goal: Determining the relationship between the incidence of pneumonia in the population of Gobi-Altai province and the common air pollutants. Material and Method: According to the rotation research model, the common external air pollutants SO₂, NO₂, PM₁₀, climate parameters, temperature, pressure, humidity, and population pneumonia measurements and registration data of Altai Sum, Gobi-Altai Province in 2020-2021 were analyzed using SPSS-24 software. statistical processing was calculated for non-parametric parameters. Result: The annual average value of sulfur dioxide (SO₂ ) measured in 20 minutes in Gobi-Altai province is 25.2 ± 13.7 μg/m³, the annual average value of nitrogen dioxide (NO₂) measured in 20 minutes is 36.329±29 μg/m³, and the annual average concentration of PM₁₀ particles is 35.2 ± 28,264 μg/m³, which is the standard level of MNS4585:2016. But the 20-minute average concentration of SO₂ (r=0.81, p=0.005), the average concentration of NO₂ (r=0.089, p=0.008), and the average concentration of PM₁₀ (r=0.089, p=0.002) is directly related to the incidence of pneumonia. It is also inversely correlated with air temperature (r=-142, p=0,000). Conclusion Common outdoor air pollutants in Yesunbulag sum, Gobi-Altai Province are related to pneumonia in children aged 0-5 years.

Introduction: The average age of the population varies in every country of the world, which depends on the changes that occur in the body during aging and many external and internal factors that affect it. Thyroid hormones play an important role in ensuring and maintaining normal metabolic function throughout life. Our country is located in a mountainous region of the mainland, the amount of iodine in drinking water is low, and the amount of iodine taken from food is low, which creates the risk of iodine deficiency disorders. Therefore, we aimed to study how the thyroid function changes with age in Mongolian people. Goal: A comparative study of thyroid hormone secretion levels in adult women. Material and Method: In our study, 83 women over the age of 20 were selected based on the “Yu Bi Lab” diagnostic center. Triiodothyronine (T3), thyroxine (T4), and thyrotropin (TSH) of the women participating in the study were determined using the FHU method using the Magiwel ELISA diagnostic kit manufactured by United Biotech. Results: 81 women over 20 years of age participated in our study, the average age was 44.45±14.17, and the participants were divided into 5 groups with 10 age intervals. 20-29 years 18% (n=15) average age 24.6±2.64, 30-39 years 22% (n=18) average age 33.6± 3.12, 40-49 years 16% (n=13) average age 45.15±1.67, 50-59 years 25% (n=20) average age 54.15±2.99, 60-69 years 19% (n=15) average age 63.73± 3.05, were. Conclusion
1. When comparing T3 and T4 hormones by age group, T3 hormone is higher in the 30-39 age group at 5.05 ng/ml. The T4 hormone gradually decreases with age.
2. Thyroiodin hormone increased with age. The relationship between the age of the participants and TSH hormone was directly related to statistical age when calculated by Pearson’s correlation coefficient (p=0.01).

Introduction : Water is a vital resource for human existence and is essential for daily food processing, preparation, washing, hygiene, and sanitation. Furthermore, providing the population with safe drinking water is one of the pressing problems of the world and some regions.
In recent years, population density and the scale of commercial and industrial activities, as well as clean and dirty water consumption were increased in the capital city. As a result of these, ground and surface water resources are becoming scarce and polluted.
Therefore, assessment of daily drinking and residential water consumption of Ulaanbaatar should be determined to use drinking water properly in daily life and water loss. This study assessed the actual amount of households’ daily water consumption. Goal: The purpose of this study is to determine the daily consumption of drinking water for households in ger areas and apartments in Ulaanbaatar. Materials and Method: This study covers 30 households in ger areas and 15 apartment households, in Ulaanbaatar. Household members performed 6-10 types of measurements every day, within 7 days. As a result of these measurements, actual consumption of water quantity used for drinking and household purposes was calculated. Statistical analysis was done by SPSS Version 21 to calculate the true probability of difference between parameters. Result: 67.9% of the households in the ger areas were 4-5 family members. The average daily household consumption of drinking and domestic water were 68.3 ± 3.57 L (95% 61.3-75.3), the minimum consumption was 12 L, and the maximum was 227 L. Average of the household water consumption water was 97.6-108.9 liters during the weekends, and water consumption was statistically higher than weekdays (p=0.001; p=0.01).
The water consumption of residential households with 3 family members accounted for the majority (30.8%) in this study. The average daily consumption of drinking and domestic water was 297.67±19.7 liters. There was no statistically significant difference (p=0.96) in week. The average daily water consumption including drinking and residential was 270.3-335.97 L.
The total daily drinking and residential water consumption per person was 15.57 L for households in ger areas and 90 L for apartment households. Calculating the daily water consumption of households in ger areas, 60.3% of it is used for laundry, washing dishes, food preparation, washing face and hands, and clothes, 31% for drinking, and 8.7% for outdoor water use. While apartment households, approximately 94.1%, were used for household and 5.9% for drinking purposes. Research ethics approval : This study was discussed at the meeting of the Academic Council of the National Center for Public Health. In addition, this study was carried out according to the methods and methods discussed and approved at the meeting of the Medical Ethics Control Committee of the Ministry of Health (Resolution No. 08). Conclusion The total daily consumption of drinking and residential water per person were 15.57 litres for the households in the ger areas and 90 litres for the apartment households. It implies that it does not exceed the WHO recommendations

Background: Toxoplasmosis is an infection caused by the parasite Toxoplasma gondii. Cats are the reservoir of the parasite. Humans can become infected either by ingesting the cysts (by direct contact with cats or through food or water contaminated by cat faeces) or by eating poorly cooked meat containing cysts. We aimed to study on knowledge, attitude, risk of Toxoplasmosis among herder in Khovd province. Materials and Methods:
<b>Study methods: b>The study used a descriptive cross-sectional design and included herders from Bulgan, Buyant, Darvi, Zereg, Myangad, Khovd, Erdeneburen, Jargalant soum, which have the largest livestock number in Khovd province. A questionnaire was administered to determine the knowledge, attitudes, and risk factors for toxoplasmosis among herders. Study ethics: Before starting the study, the methodology was discussed at a meeting of the Ethics Committee of “Ach” University of Applied Sciences, and the research (Resolution No. 23/02) was approved. Data processing: The data collected during the study were coded into Microsoft Office Excel and a database (file) was created. Open Epi Info and SPSS 20 were used to statistically process the results of the study (mean, standard deviation, relative risk). Result: In result, 180 herder’s average age was 48.1±17.8 years old, most of them were female. Most of herder were herding their livestock over six years. During their herding period, 31% of herder household has registered rabies, anthrax, brucellosis, swine fever, blood infection, blindness, smallpox, horse pox, scabies, foot and mouth disease among their livestock. By questionnaire, toxoplasmosis has not registered among livestock. When assessing self protection practices when dealing with suspected infected animals, 14-22.3% use masks and aprons when dealing with sick animals, 1.4-3% use goggles, coveralls, and disinfectants, and the lowest percentage is 0.2% of gloves. This indicates a very high risk of transmission of zoonotic diseases among herder of Khovd province. Conclusion In our study, although no cases of toxoplasmosis were recorded among livestock in the soums of the Khovd province by questionarie, the lack of self-protection practices and attitudes against animal-to-human transmission and the high risk of infection indicate the importance of disseminating information and organizing training on zoonotic diseases

Abstract: In Mongolia, flu and flu-like diseases affect many people, and pneumonia is a public health concern. Therefore, we have an urgent need to research and develop a new medicinal syrup to replace imported products using raw materials grown in Mongolia and to compare it with similar products. There are imported cough syrup products on the pharmaceutical market of Mongolia, but there are domestic pharmaceutical products. Moreover, there is limited research data on cough relief. The main goal of the research team is to compare the experimentally obtained syrup containing Plantago asiatica L, Malva Sylvestris L. and syrup containing Plantago major L. Juice, honey that produced by Monos Pharm LLC whish the cough relieving drug Gerbion syrup. Material and methods: The vivarium of the pharmacology laboratory, Drug research institute, TPC7062Ti mark IVC system (Shanghai Pretty Industries Co.,Ltd, China) in a special environment with one care and one feeding regimen (Lab Mice Diet, Constant Formula Balanced Nutrition, Jiangsu Xietong Pharmaceutical Bio- Engineering Co., LTD, China, www.jsxtsw.com ) used 64 non-WISTAR white rats weighing 180-218 g. Experimental rats were smoked with an aerosol of 17% citric acid aqueous solution for 5 minutes. The statistical processing of the research results was done using the One-Way ANOVA/Multiple comparisons data of the GraphPad Prism 8 program. Results and conclusion Expressing the number of coughs produced in the experimental rats as a percentage (%), the control group showed 3.95% cough relief, and the experimental group-1 by 48.4%, experimental group-2 by 71.1%, comparison group by 74.6%, respectively has reduced. Experimental group -2 and comparison group for dry cough caused by citric acid 17%, it affects the cough receptors in the mucous membrane of the respiratory tract and relaxes the bronchial muscles (A and C fiber).

Introduction: This field assessment was performed to ensure the effective implementation of Order No. A/142 of the Deputy Prime Minister of Mongolia dated 30^th December 2021 and Order No. A/01 of the Head of the National Committee for Reducing Environmental Pollution dated 3^rd January 2022 and to monitor the level of indoor air pollutants emitted from traditional stoves and standard stoves. Purpose: To determine the level of indoor air pollutants emitted from briquette combustion and to develop recommendations by comparing various types of stoves and dwellings. Materials and Methods: Through analytical cross-sectional designs, three households from Songinokhairkhan district and one household from Chingeltei district were selected to participate in this assessment as they use briquettes for heating, vary in their dwelling types (ger (traditional Mongolian tent), house) and their stove types (traditional, standard), stay home during the day and chose to participate in the assessment. 24-hour continuous measurements were conducted for each dwelling and before taking measurements, we calibrated and adjusted the air suction velocity of the measuring instrument at the Central Metrology Laboratory. The monitor was placed at a distance of 0.5m from the stove and a height of 0.5-1.0m from the floor. The results were then compared with the Mongolian National Standard “General air quality and technical requirements MNS4585:2016” and WHO Guidelines for Indoor Air Quality 2021. Statistical analysis was performed using SPSS 24.0 software, mean, ANOVA test, and if the p-value is less than 0.05, our results are statistically significant. Results: The concentration level of SO₂ in houses with “Dul” stoves was within the level specified in the Mongolian standard MNS4585: 2016 and WHO Guidelines for Indoor Air Quality 2021, while 24-hour SO₂ concentration in gers and houses with traditional stoves was 1.3-2.92 times higher than the standards. The 24-hour average concentration of nitrogen dioxide in houses with traditional stoves was 1.73 times higher than the Mongolian air quality standard MNS4585: 2016. There were statistically significant differences in the emission levels of SO₂ and NO₂ depending on dwelling and stove types (df=4, f=4.04, p=0.008 and df=4, f=17.17, p=0.001, respectively.). The 8-hour average (10,000 μg/m3) CO concentration was statistically significant for various types of dwellings and stoves (df=4, f=45.17, p=0.001), whereas there was a statistically significant difference in CO concentrations in gers with traditional stoves and gers with standard stoves in terms of morning, afternoon, and evening hours (df=3, f=33.17, p=0.001). Conclusion Indoor air pollutants in the households except for houses with “Dul” stoves have been determined to exceed the air quality standards set by the Mongolian air quality standards and WHO Guidelines for Indoor Air Quality 2021.