Background: Type I hypersensitivity, commonly known as an allergic reaction, is characterised by an IgE-mediated immune response triggered when foreign proteins enter the body. During allergic reactions, cytokines such as IL-4, IL-5, IL-9, and IL-13, secreted by T-helper 2 (Th2) cells, play a crucial role in activating immune effector cells like eosinophils and mast cells, which contribute significantly to airway inflammation and obstruction. Conversely, interferon-gamma (IFN-γ), produced by Th1 cells, suppresses Th2-mediated responses and plays an essential role in regulating allergic inflammation. Therefore, determining the balance of cytokines produced by Th1 and Th2 cells is crucial for understanding the mechanisms underlying allergic responses. Aim: We aimed to induce an allergic response in experimental animals using ovalbumin and to determine the levels of IL-4 and IFN-γ cytokines using an in-house enzyme-linked immunosorbent assay (ELISA). Materials and Methods: A total of 20 female C57BL/6 mice aged 6–8 weeks were selected for the experiment and randomly divided into two groups: control and experimental groups. The concentrations of IL-4 and IFN-γ cytokines in mouse serum were determined using an in-house enzyme-linked immunosorbent assay (ELISA). Result: Compared to the control group, the levels of IL-4 and IFN-γ cytokines in the serum of allergic model-induced C57BL/6 mice were determined to be 513.7 pg/mL and 193.2 pg/mL, respectively. A statistically significant difference was observed in IL-4 cytokine levels between the two groups (p < 0.001). Conclusion In the allergic model group, serum levels of IL-4 cytokine were elevated, while IFN-γ cytokine levels were decreased, suggesting a predominant Th2-type immune response.

Background: The cornerstone of medical education in training qualified physicians lies in foundational scientific knowledge, which forms the basis for clinical reasoning and diagnostic-therapeutic decision-making. Anatomy constitutes one of the core foundational subjects within the medical doctor training curriculum and exerts a direct influence on the development of students’ baseline knowledge and the formation of their clinical thinking. This study was conducted to evaluate the anatomy curriculum through assessments by first-year students enrolled in the basic medical education program, with the aim of enhancing instructional quality and improving the structure and organization of the course. Aim: To investigate the satisfaction ratings of first-year students in the basic medical education program regarding the Anatomy-1 course. Material and Methods: The study was carried out using an analytical cross-sectional design during the first semester of the 2025–2026 academic year. Data were collected from a total of 289 first-year students of the basic medical education program at the School of Bio-Medicine of MNUMS (Mongolian National University of Medical Sciences) using a questionnaire comprising 24 items in total—3 demographic questions, 20 general satisfaction questions, and 1 open-ended question—with each item rated on a 5-point Likert scale. Data were processed using SPSS 23.0 software, employing descriptive statistics, two-sample t-tests, ANOVA, Pearson’s correlation, and factor analysis. Results: Of the study participants, 14.2% were male and 85.8% were female, with the cohort distributed across programs as follows: TAM 10.38%, TMS 17.30%, MDS 44.29%, PDS 13.15%, and DSS 14.88%. The overall satisfaction level was 4.59±0.66, falling within the “good” range. The highest rating was attributed to “Faculty members possess strong professional knowledge and skills” (DSS, 4.91±0.37), whereas the lowest rating was assigned to “Students’ knowledge and skills are evaluated objectively and fairly” (PDS, 4.16±0.75). The internal consistency coefficient of the questionnaire yielded α=0.909, indicating excellent reliability. Correlation analysis revealed strong positive associations among instructors’ respect for students’ viewpoints (Q4), instructors’ communication and demeanor (Q5), and professional ethics (Q6) (r=0.787; r=0.755; p<0.01). Factor analysis produced a KMO value of 0.865 and a Bartlett’s test result of p=0.000; accordingly, all 20 items were grouped into two factors—”Faculty Professional Competence” and “Learning Environment and Organization”—which collectively accounted for 58.74% of the total variance. Conclusions 1. The findings indicate that the overall satisfaction of first-year basic medical education students who participated in the study was at a favorable level. 2. Faculty professional competence and the learning environment and organization were identified as factors influencing student satisfaction, explaining 58.74% of the total variance in the results. It was further established that adherence to professional ethics, respect for students’ perspectives, and equitable treatment are contingent upon the instructor’s knowledge and professional expertise.

Background: The Anatomy course, which provides general foundational knowledge on the structure of all organ systems of the human body, is a core professional course in the bachelor’s curricula of Pharmacy, Biomedical Science, Hygiene and Epidemiology, Public Health, Occupational Safety and Health, and Nutrition and Dietetics. Student satisfaction is not only an important indicator of the quality of medical education, but also influences graduates’ knowledge, skills, and attitudes; therefore, continuous research in this area is necessary. Aim: To determine the level of satisfaction with the Anatomy curriculum among first-year students in basic health science programs and to identify factors influencing their satisfaction, with the aim of improving the quality of teaching. Materials and Methods: An analytical cross-sectional study was conducted from September 2025 to February 2026 among 182 first-year students enrolled in the following programs at Mongolian National University of Medical Sciences (MNUMS): Biomedical Research, Pharmacy, Health Epidemiology, Public Health, Health Informatics, and Traditional Medicine Therapy. Student satisfaction with the quality of the Anatomy course was assessed using a 24-item questionnaire. Data were analyzed using SPSS version 26, applying descriptive statistics, independent-samples t-test, one-way analysis of variance (ANOVA), Pearson correlation, factor analysis, and multiple regression analysis. A p-value of <0.05 was considered statistically significant. Results: The internal consistency of the satisfaction scale was very high (Cronbach’s α=0.928). The mean overall satisfaction score of the participants was 4.61±0.67. There was no significant difference in overall satisfaction by gender (p > 0.05). Factor analysis identified two main factors influencing the evaluation of teaching quality (KMO=0.894; Bartlett’s test p<0.001), which together explained 52.73% of the total variance. The first factor was “Teachers’ professional competence, communication, attitude, and assessment,” and the second factor was “Course organization, learning environment, and instructional materials.” Correlation analysis showed that better course organization and higher teacher engagement were positively associated with students’ satisfaction (p<0.01). Conclusions 1. Among first-year basic health science students, satisfaction with the Anatomy course was influenced by teachers’ professional competence, ethics, interpersonal communication, and fair, objective assessment. 2. Adherence to professional ethics and maintaining respectful communication with students were found to be closely related to teachers’ knowledge and professional skills.

Background: The study of the anatomical structure of the ankle joint ligaments is essential for understanding the stability, axis, and movement of the joint, as well as their interrelationship with the function. It also provides fundamental quantitative data for the training and research of physiotherapists, as well as the practice of injury and trauma medicine. Proper diagnosis and treatment of ankle joint injuries, as well as understanding the mechanism of injury, are based on this knowledge. Ankle joint injuries are common, with ligament and tendon injuries accounting for 85% of ankle injuries. In Mongolia, 81.1% of ankle injuries are new, 9.4% are similar to the initial injury, 8.1% are recurrent injuries, and 0.7% have unknown causes. Depending on the mechanism of injury, certain ligaments and connective tissues can be damaged. Ankle ligament injuries are the most common cause of acute ankle pain, and chronic ankle pain is often due to structural changes in the ankle ligaments. Aim: To summarize the research on the normal anatomy of the ankle and subtalar joint, as well as the studies on joint injuries, using the dissection method. Materials and Methods: Literature search was conducted in Thomson Reuters, Pubmed central database, Google Scholar, and Web of Science databases, as well as the statistical data from the Health Development Center and the National Center for Trauma and Orthopedics. Keywords used include: ankle joint, subtalar joint, ankle joint and subtalar joint injuries. A total of 330 publications were identified, and 34 key articles on the normal anatomy of the ankle and subtalar joint, as well as joint injuries, were included in the review. Results: The ankle joint is formed between the distal end of the tibia and the superior articular surface of the talus. It allows plantar flexion and dorsiflexion movements, with a normal range of 30 degrees for plantar flexion and 50 degrees for dorsiflexion. The subtalar joint is formed between the posterior articular surfaces of the talus and calcaneus. It allows inversion and eversion movements, with a normal range of 35 degrees for inversion and 20 degrees for eversion. The ankle ligaments are divided into three groups: lateral, medial, and inferior tibiofibular (syndesmotic) ligaments. The most common cause of ankle joint injuries is excessive inversion of the subtalar joint, leading to injuries to the joint capsule, lateral and medial collateral ligaments, and the syndesmotic ligaments. Excessive plantar flexion and dorsiflexion of the ankle joint can also cause injuries to the posterior talofibular ligament, the osteochondral region of the talar neck, and the anterior-inferior margin of the tibia. Conclusion Comprehensive study of the ankle joint structure provides the basis for explaining the mechanism of injury. Further research in Mongolia is needed, combining dissection and imaging techniques, to develop standardized evaluation protocols that correlate with clinical outcomes.

Background: Insects are an inexhaustible source of medicine, and it is essential to study the biologically active substances contained in insect bodies. Although there is information about various insects and beetles used in traditional Mongolian medicine treatment, there are few studies that have compiled and compared these at the source level. Aim: To study and determine from primary sources information about the composition of pheromone secretions contained in the bodies of various insects and worms used in “Entomotherapy (Insect therapy)” in traditional medicine, particularly rare beetle species, and information about what diseases and ailments these insects were used to treat. Materials and Methods: This study used primary source and historical research methods, analysis-synthesis methods, systematic analytical methods, and historical comparative methods. Information was extracted from traditional medicine source books about insect therapy, particularly identifying and compiling primary sources about the use of beetles in folk medical treatment. Results: According to primary sources, traditional Mongolian medicine used quite a variety of “therapeutic insects and worms” of animal origin, including representatives of arthropods such as scorpions, spiders, centipedes, wasps, bees, various types of beetles, and moths. Among these, stag beetles, dung beetles, and black beetles were used in traditional medicine for treating joint and muscle diseases, various women’s diseases, epilepsy and convulsions, headaches, and all 11 types of bile diseases, with specific prescriptions developed for each condition. Conclusion Traditional medicine treatment used various insects from the biological classification of the insect class, particularly many types of beetles from the Coleoptera order. The pheromone secretions contained in their bodies include many different types of chemical substances.

Background: In Mongolian Traditional Medicine (MTM), the human body constitution is classified according to the doctrine of Khii, Shar, and Badgan, resulting in seven types: three single constitutions Khii, Shar, Badgan, three mixed constitutions Khii-Badgan, Shar-Badgan, Khii-Shar, and one combined constitution in which all three principles coexist. This concept considers not only physical, physiological, mental, and behavioral characteristics but also environmental influences. Childhood obesity has become a major global public health issue. In Mongolia, the prevalence of overweight and obese children aged 5–19 years is projected to reach 19% (approximately 79,648 children) by 2030. However, limited research has examined the relationship between children’s constitutional types and their physical and body composition indicators. Aim: Children (11-17 ages) innate nature of and study of the relationship between physical and body composition. Materials and Methods: A cross-sectional analytical study was conducted among 459 students aged 11–17 years from “Erdmiin Khutuch” Secondary School No. 58, Sukhbaatar District, Ulaanbaatar, selected through random sampling. Constitutional types were determined using the “Mongolian Traditional Medicine Human innate nature Typology” developed by B. Dagvatsereen and U. Batchimeg (2003), based on 19 parameters and 44 indicators through a web-based tool (WEB1). Body composition was measured using a Tanita DC430a analyzer. Statistical analyses were performed using SPSS version 27. Results: Among children aged 11-13 years, the predominant constitutional types were Badgan-Shar, Khii-Badgan, Khii- Shar, Badgan-Khii, and Shar-Khii, with Badgan-Khii being the most common. In those aged 14-17 years, the dominant constitutions shifted to Shar–Khii, Khii–Shar, and Shar–Badgan, with Shar–Khii and Khii–Shar more frequently observed. These patterns may be related to the physiological characteristics of growth during childhood and adolescence. The average height of boys was 158.35±1.13 cm (118-175), while that of girls was 154.94±0.61 cm (116-175), indicating that boys were 3.41 cm taller than girls (p<0.05). The mean waist circumference of boys was 69.66±0.79 cm (60-110), compared to 67.77±0.66 cm (29.50-108) in girls, showing a statistically significant difference of 1.89 cm (p<0.05). The findings demonstrated that girls had higher body fat percentage and fat mass than boys, whereas boys exhibited greater skeletal muscle percentage, skeletal mass, muscle percentage, and muscle mass. These sex differences in fat and muscle composition among children aged 11-17 years may be attributable to hormonal variations associated with pubertal development. Body mass index, waist circumference, body fat percentage, visceral fat level, daily metabolic expenditure, obesity grade, and bone mass showed a weak inverse correlation with the Khii–Shar and Shar–Khii constitutional types. Conclusions 1. Among children aged 11-13 years, Badgan-Shar, Khii-Badgan, Badgan-Khii, and Shar-Khii constitutions were dominant, while in those aged 14-17 years, Shar-Khii, Khii-Shar, and Shar-Badgan types predominated. 2. When comparing children’s physical indicators by sex, boys have greater height and waist circumference than girls. Regarding body composition, girls show higher body fat percentage and fat mass, whereas boys have greater skeletal muscle mass, muscle percentage, and total muscle mass compared to girls. 3. The body mass index, waist circumference, body fat percentage, and degree of obesity showed a weak positive correlation with the Badgan-dan and Badgan-khii constitutional types. In contrast, skeletal muscle mass, muscle weight, body water percentage, and body protein percentage demonstrated a weak positive correlation with the Khii-shar and Shar-khii constitutional types.

BACKGROUND. As long ago times or perhaps longer, people were using insects as medicines for healing wounds, preventing infections and improving health. Some of these are purely anecdotal, while others have proven basis in fact as tested by modern medicine.Usage of insects intraditional medicine was recorded since time immemorial.Insects and their substances have been used as medicinal resources by different cultures since ancient time because of chemical compounds - e.g. pheromones, defensive sprays, venoms and toxins, which were sequestered fromplants or prey and later concentrated or transformed for their own use.In many parts of the world,different sections of the society have been using medico-entomological drugs to this day in their lives.A numberof studies has in recent years drawn attention to thetherapeutic value of certain species of insects, their products, and their developmental stages.As has been documented insects can be a source of drugs used in modern medicine, since compounds of insect origin can have immunological, analgesic, antibacterial, diuretic, anaesthetic and anti-rheumatic, antitumor properties. Numerous insect originated materia medicain Mongolian traditional medicine contribute this source of therapeutics and variety of ancientmedical treatises by local authors as well as translations of renowned Ayurvedic medical books about animals as medicine exist.Knowledge about therapy with insects in Mongolian traditional medicine is less studied even they have been used broadly since ancient time. Several orthodox practitioners have surveyed the therapeutic potentials of defensive agents in dark beetleknown as “stink beetle” in the past.Yet the scientific community has to give thismajor and crucial component of traditional Mongolian medicine the attention it deserves, scientific knowledge about biologically active principles within medicinal insect remain poorly unknown. AIM OF STUDY.To define chemical analysis of ethanol whole body extract of Tenebrionid beetles. METHOD AND RESULT. We collected Tenebrionid beetles from local regions including desert, grassland, and to make an 40% ethanol extract of whole body to determine species of Tenebrionid beetles by entomoscope. After 30 days for saving in organic extract, to determine chemical composition of filtered 2 ml sample solution by high performance liquid chromatography - mass spectrometry (HPLC-MS). Using digital usb microscope 2.0 mp to confirm special characters of Tenebrionid beetles to Tenebrionid B.miliaria in biological termin. In HPLC-MS, octadecanoic acid is presented in 2 regional samples. CONCLUSION. Octadecanoic acid, the surface lipid of the insect was determined from the ethanol whole body extract of Tenebrionid beetles.