This study aimed to identify factors influencing long-term care hospital nurses’ moral distress and job satisfaction on turnover intention. Methods: Participants were 158 nurses working in one of 13 long-term care hospitals. Data were collected from July 22 to September 10, 2024, and analysed using two-stage multiple regression analysis with SPSS. Results: Turnover intention was a statistically significant positive correlation with moral distress. In the sub factors, there was correlation with futile care, nursing practice, institutional and situational factors, and physician practice. Turnover intention was a statistically significant negative correlation with job satisfaction and all sub factors. Factors influencing the turnover intention of long-term care hospital nurses were under the age of 40, male nurses, moral distress, futile care sub factors of moral distress, job satisfaction, and administration and professionalism sub factors of job satisfaction, showing 30.0% explanatory power. Conclusion: The significance of moral distress and job satisfaction was confirmed as a factor influencing the turnover intention of long-term care hospital nurses. Therefore, the organization must make active efforts to find and solve factors and problems that reduce job satisfaction and intensify moral distress of long-term care hospital nurses. In addition, at the organizational level, a management strategy will be needed to lower nurses’ intention to turnover by preparing and applying ethical standards and guidelines to systematically solve the moral distress of nurses in the organization.

【Objective】 To investigate the effect of serological methods for eliminating the interference of warm autoantibodies with the compatibility test before blood transfusion. 【Methods】 The blood samples (whole blood and serum, 3 mL/each) of 10 cases of warm autoantibodies interfering with antibody screening and identification were collected. Autogenous or allogeneic red blood cells(RBCs) were treated with microthermal(45 ℃), chloroquine, or ZZAP (dithiothreitol and papain) reagents, and then were used to absorb the autoantibodies in patients′ plasma. The plasma after absorption and RBC elution were used for antibody identification by anti-human globulin method or Polybrene method to determine the specificity of the autoantibody/alloantibody. Blood transfusion with ABO/Rh homotypic RBCs without corresponding antigens was performed in patients with alloantibodies or specific autoantibodies, and the efficacy of blood transfusion was evaluated. 【Results】 The interferenc of warm autoantibodies with antibody screening and identification due to primary or secondary autoimmune diseases were eliminated after absorption, and IgG isospecific antibodies (anti-E, anti-Jk^a, anti-Wr^a) in 3 cases and specific autoantibodies (anti-Ce) in 1 case were yielded. 6 of the 10 patients received blood transfusion, and 4 with specific antibodies avoided exposure to corresponding antigens. After transfusion of 2U suspended RBCs, the increase of hemoglobin at 24h in all 6 patients was greater than 10 g/L, and no hemolytic transfusion reaction occurred. Anemia symptoms were improved after transfusion. 【Conclusion】 Appropriate elution methods and autologous/allogeneic absorption methods can eliminate the interference of warm autoantibodies with the identification of alloantibodies, therefore can accurately identify the types and properties of antibodies, thus providing targeted and effective blood transfusion.