Objective To investigate the effect of Lianpu Yin on duodenal microinflammation in rats with functional dyspepsia(FD)by regulating NLRP3 activation.Methods Wistar rats were randomly divided into blank group and model group.FD rats were reconstructed by iodoacetamide method(2%sucrose solution containing 0.1%iodoacetamide),and the model was verified.FD model rats were randomly divided into model group,Lianpu Yin group and Moxapride group by random number expression method.After a period of two weeks of administration,measurements were taken to determine the body mass,three-hour food consumption,as well as the rates of gastric emptying and intestinal propulsion.The pathological structure of duodenal tissue was observed by HE staining.The serum levels of IL-1β and IL-18 were quantified using the enzyme-linked immunosorbent assay(ELISA)method.The expression levels of NLRP3 and Caspase-1 in each group were detected by Western blot.Expression levels of NLRP3 and Caspase-1 proteins were detected by immunofluorescence.Results Compared with the blank group,body weight,food intake at 3 h,gastric emptyand intestinal propulsion rate in model group were significantly decreased(P<0.01),and inflammatory infiltration of duodenum tissue appeared in the model group.Meanwhile,the expressions of NLRP3 and Caspase-1 proteins,as well as the levels of IL-1β and IL-18 in the duodenal tissue of the model group,showed significant increasing(P<0.05).Compared with the model group,rats in the Lianpu Yin and Moxapride groups displayed significant increasing in body weight,gastric emptying rate,and intestinal propulsion rate(P<0.01).Additionally,inflammatory infiltration of duodenum tissue reduced in these groups.Furthermore,NLRP3 and Caspase-1 protein expressions,as well as IL-1β and IL-18 levels,significantly decreased in the Lianpu Yin and Moxapride groups compared to the model group(P<0.05).Conclusion Lianpu Yin can treat FD rats by inhibiting duodenal microinflammation and then restoring gastrointestinal motility,which may be related to the abnormal activation of NLRP3 inflammasome.

Objective To use Citespace 6.2.R4software to analyze the literature research on the prevention and treatment of HP-associated gastritis by traditional Chinese medicine in recent 10 years,and to discuss the research hotspot and research trend in this field.Methods Literature on the treatment of Helicobacter pylori(Hp)associated gastritis with traditional Chinese medicine was retrieved from CNKI database,and then these literature datas were imported into Citespace knowledge graph software,which was used to visually analyze the number of publications,author distribution,research institutions and keywords in the literature.Results A total of 930 literatures were included.The number of published papers from 2015 to 2024 was basically stable,and the number of published papers from 2019-2020 was the largest;Author co-occurrence analysis shows that the authors with the most publications are Liu Qian,Yu Bin and Zhou Yifang.The co-occurrence analysis of institutions showed that the institutions with the highest number of publications were Nanjing University of Chinese Medicine,Hunan University of Chinese Medicine,Guangzhou University of Chinese Medicine and Hubei University of Chinese Medicine.Keyword analysis shows that the current research in this field mainly focuses on clinical efficacy,clinical research,TCM syndrome types,famous doctors'experience,data mining and so on.Conclusion In recent years,Chinese medicine has shown a high popularity in the prevention and treatment of HP-related gastritis.In the future,cross-regional exchanges and cooperation between various teams and institutions should be strengthened,more real-world studies on TCM prevention and treatment of HP-associated gastritis should be carried out,high-level evidence-based evidence should be obtained,and the mechanism of action of the prescription should be clarified.

Objective To use Citespace 6.2.R4software to analyze the literature research on the prevention and treatment of HP-associated gastritis by traditional Chinese medicine in recent 10 years,and to discuss the research hotspot and research trend in this field.Methods Literature on the treatment of Helicobacter pylori(Hp)associated gastritis with traditional Chinese medicine was retrieved from CNKI database,and then these literature datas were imported into Citespace knowledge graph software,which was used to visually analyze the number of publications,author distribution,research institutions and keywords in the literature.Results A total of 930 literatures were included.The number of published papers from 2015 to 2024 was basically stable,and the number of published papers from 2019-2020 was the largest;Author co-occurrence analysis shows that the authors with the most publications are Liu Qian,Yu Bin and Zhou Yifang.The co-occurrence analysis of institutions showed that the institutions with the highest number of publications were Nanjing University of Chinese Medicine,Hunan University of Chinese Medicine,Guangzhou University of Chinese Medicine and Hubei University of Chinese Medicine.Keyword analysis shows that the current research in this field mainly focuses on clinical efficacy,clinical research,TCM syndrome types,famous doctors'experience,data mining and so on.Conclusion In recent years,Chinese medicine has shown a high popularity in the prevention and treatment of HP-related gastritis.In the future,cross-regional exchanges and cooperation between various teams and institutions should be strengthened,more real-world studies on TCM prevention and treatment of HP-associated gastritis should be carried out,high-level evidence-based evidence should be obtained,and the mechanism of action of the prescription should be clarified.

Objective To investigate the effect of Lianpu Yin on duodenal microinflammation in rats with functional dyspepsia(FD)by regulating NLRP3 activation.Methods Wistar rats were randomly divided into blank group and model group.FD rats were reconstructed by iodoacetamide method(2%sucrose solution containing 0.1%iodoacetamide),and the model was verified.FD model rats were randomly divided into model group,Lianpu Yin group and Moxapride group by random number expression method.After a period of two weeks of administration,measurements were taken to determine the body mass,three-hour food consumption,as well as the rates of gastric emptying and intestinal propulsion.The pathological structure of duodenal tissue was observed by HE staining.The serum levels of IL-1β and IL-18 were quantified using the enzyme-linked immunosorbent assay(ELISA)method.The expression levels of NLRP3 and Caspase-1 in each group were detected by Western blot.Expression levels of NLRP3 and Caspase-1 proteins were detected by immunofluorescence.Results Compared with the blank group,body weight,food intake at 3 h,gastric emptyand intestinal propulsion rate in model group were significantly decreased(P<0.01),and inflammatory infiltration of duodenum tissue appeared in the model group.Meanwhile,the expressions of NLRP3 and Caspase-1 proteins,as well as the levels of IL-1β and IL-18 in the duodenal tissue of the model group,showed significant increasing(P<0.05).Compared with the model group,rats in the Lianpu Yin and Moxapride groups displayed significant increasing in body weight,gastric emptying rate,and intestinal propulsion rate(P<0.01).Additionally,inflammatory infiltration of duodenum tissue reduced in these groups.Furthermore,NLRP3 and Caspase-1 protein expressions,as well as IL-1β and IL-18 levels,significantly decreased in the Lianpu Yin and Moxapride groups compared to the model group(P<0.05).Conclusion Lianpu Yin can treat FD rats by inhibiting duodenal microinflammation and then restoring gastrointestinal motility,which may be related to the abnormal activation of NLRP3 inflammasome.