1.SEROLOGICAL AND MOLECULAR SURVEY OF HEMOPLASMAS IN PIGS REVEALS PREVALENCE OF MYCOPLASMA PARVUM IN HAINAN ISLAND, CHINA
Kadam AMIT ; Jin-Xiu WANG ; Gao-Jin XIE ; Kai-Gong WANG ; Wei WANG ; Jing-Yi ZHAO ; Jia-Min WU ; Ci-Han YANG ; Jin-Hua WANG ; Cheng-Hong LIAO ; Nielsen Peter JENS ; Qian HAN
Acta Parasitologica et Medica Entomologica Sinica 2018;25(3):140-147
Mycoplasma suis and M.parvum are epierythrocytic prokaryotes and causative agents of porcine eperythrozoonosis.Its infections in pigs from Hainan Island,China have not been previously reported.The objective of this study was to investigate the prevalence of Mycoplasma spp.in pigs in Hainan Island,China.The study includes PCR amplification and sequencing using universal primers for 16S rRNA and 23S rRNA genes and further phylogenetic analysis.A total 593 samples were collected and tested for M.suis and M.parvum by end point PCR,real-time PCR and a kit based on immuno-chromatographic assay.The results showed that 6.4％ (15/233) pigs from Hainan Island,China were found positive with M.parvum.This study raises an interesting question regarding the probability of different endemic Mycoplasma species presence in the Island when compared to the Mainland China (Beijing,Guizhou and Fujian provinces with 2.1％ (2/95),0.4 ％ (1/218) and 4.2％ (2/47),respectively),where only M.suis has been found in this study.Out of three diagnostic tests we used here,we found that real-time PCR and immunochromatography are more sensitive than the end point PCR for detection of Mycoplasma spp.from pig blood samples.Our present study describes that M.parvum is present in pigs from Hainan.This raises a need for further studies to understand the economic importance,evolution and transmission of the species.
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