2.Simultaneous determination of three coumarin constituents in roots of Peucedanum praeruptorum by RP-HPLC.
Wenling WU ; Jiajia CHEN ; Shoujin LIU ; Nianjun YU ; Chengwu FANG ; Qingqing ZHANG
China Journal of Chinese Materia Medica 2009;34(9):1121-1123
OBJECTIVETo develop a reserved-phase HPLC method for the determination of praeruptorin A, praeruptorin B, qianhucoumarin E in roots of Peucedanum praeruptorum.
METHODAgilent TC-C18 column (4.6 mm x 250 mm, 5 microm) was used at 30 degrees C with the mobile phase of methanol-water (75:25). The flow rate was set at 0.8 mL x min(-1). The detection wavelength was 321 nm.
RESULTThe linear response ranged from 3.20-28.80 microg for +/- praeruptorin A (r = 0.9999, n = 5), 1.60-14.40 g for praeruptorin B (r = 0.9995, n = 5) and 1.64-14.76 g for qianhucoumarin E (r = 0.9994, n = 5), respectively. Recoveries were 98.92% with RSD 1.6% for praeruptorin A, 99.66% with RSD 1.5% for praeruptorin B and 99.72% with RSD 1.4% for qianhucoumarin E.
CONCLUSIONThe method is quick, simple and repeatable for determination of three coumarin constituents in root of P. praeruptorum.
Apiaceae ; chemistry ; Chromatography, High Pressure Liquid ; Coumarins ; analysis ; isolation & purification ; Linear Models ; Plant Roots ; chemistry ; Reproducibility of Results ; Time Factors
Result Analysis
Print
Save
E-mail