Polycystic ovary syndrome（PCOS） and its resulting infertility is one of the common diseases of gynecology and reproductive endocrinology. The phosphatidylinositol 3-kinase/protein kinase B（PI3K/Akt） signaling pathway is relatively well-studied in the development of intervention in PCOS， and the experiments on PCOS in rats conducted by traditional Chinese medicine through this signaling pathway is also the main direction of mechanistic research. In this paper， 20 articles published in academic journals in the past 5 years were selected through the corresponding criteria， and the objective situation and existing problems of the selected research projects were analyzed from five aspects， namely， baseline data， modeling and treatment， grouping， evaluative indexes， and pharmacodynamic indexes. It is found that there were different degrees of problems in each research project， such as the observation indicators of modeling， criteria for judging the success of the model， the treatment period， the calculation of dosage of prescription/active ingredients and specific dosage were not clearly defined， which could easily lead the bias of the results or reduce the validity of experimental data. Based on this， the list of PCOS rat experimental research operations was formed， involving five categories of experimental rats， model construction， study implementation， outcome measures and analysis and report with a total of 21 operation lists， with a view to provide a reference for the subsequent PCOS experiments related to scientific research and helping to form high-quality results.

ObjectiveTo construct a large language model （LLM）-based intelligent pre-consultation system for traditional Chinese medicine （TCM） to improve efficacy of clinical practice. MethodsA TCM large language model was fine-tuned using DeepSpeed ZeRO-3 distributed training strategy based on YAYI 2-30B. A weighted undirected graph network was designed and an agent-based syndrome differentiation model was established based on relationship data extracted from TCM literature and clinical records. An agent collaboration framework was developed to integrate the TCM LLM with the syndrome differentiation model. Model performance was comprehensively evaluated by Loss function， BLEU-4， and ROUGE-L metrics， through which training convergence， text generation quality， and language understanding capability were assessed. Professional knowledge test sets were developed to evaluate system proficiency in TCM physician licensure content， TCM pharmacist licensure content， TCM symptom terminology recognition， and meridian identification. Clinical tests were conducted to compare the system with attending physicians in terms of diagnostic accuracy， consultation rounds， and consultation duration. ResultsAfter 100 000 iterations， the training loss value was gradually stabilized at about 0.7±0.08， indicating that the TCM-LLM has been trained and has good generalization ability. The TCM-LLM scored 0.38 in BLEU-4 and 0.62 in ROUGE-L， suggesting that its natural language processing ability meets the standard. We obtained 2715 symptom terms， 505 relationships between diseases and syndromes， 1011 relationships between diseases and main symptoms， and 1 303 600 relationships among different symptoms， and constructed the Agent of syndrome differentiation model. The accuracy rates in the simulated tests for TCM practitioners， licensed pharmacists of Chinese materia medica， recognition of TCM symptom terminology， and meridian recognition were 94.09%， 78.00%， 87.50%， and 68.80%， respectively. In clinical tests， the syndrome differentiation accuracy of the system reached 88.33%， with fewer consultation rounds and shorter consultation time compared to the attending physicians （P＜0.01）， suggesting that the system has a certain pre- consultation ability. ConclusionThe LLM-based intelligent TCM pre-diagnosis system could simulate diagnostic thinking of TCM physicians to a certain extent. After understanding the patients' natural language， it collects all the patient's symptom through guided questioning， thereby enhancing the diagnostic and treatment efficiency of physicians as well as the consultation experience of the patients.

Aiming at the problems of tongue diagnosis instruments relying on manual operation,uneven illumination,color distortion and lack of dynamic information,an automated operation black box tongue diagnosis instrument based on machine vision is proposed.This instrument utilizes machine vision technology and image processing algorithms to achieve automatic positioning and automatic acquisition of tongue manifestations.High-resolution camera technology enables the acquisition of 3-second dynamic videos and high-quality static images.The performance test results show that,based on the dual-camera geometric projection calibration algorithm,the average time for tongue recognition is less than 0.15 seconds.The diffuse reflection light source improves the gray uniformity to 76.74%,and the maximum color difference in color reproduction degree is 19,which is better than the industry standard value of 25.The developed tongue diagnosis instrument will promote the objective,efficient and precise acquisition of tongue diagnosis in traditional Chinese medicine.

Aiming at the problems of tongue diagnosis instruments relying on manual operation,uneven illumination,color distortion and lack of dynamic information,an automated operation black box tongue diagnosis instrument based on machine vision is proposed.This instrument utilizes machine vision technology and image processing algorithms to achieve automatic positioning and automatic acquisition of tongue manifestations.High-resolution camera technology enables the acquisition of 3-second dynamic videos and high-quality static images.The performance test results show that,based on the dual-camera geometric projection calibration algorithm,the average time for tongue recognition is less than 0.15 seconds.The diffuse reflection light source improves the gray uniformity to 76.74%,and the maximum color difference in color reproduction degree is 19,which is better than the industry standard value of 25.The developed tongue diagnosis instrument will promote the objective,efficient and precise acquisition of tongue diagnosis in traditional Chinese medicine.

A 24-year-old male patient with viral meningoencephalitis received intravenous infusion of acyclovir 0.5 g thrice daily,Xingnaojing injection（醒脑静注射液）20 ml once daily,mannitol injection 250 ml thrice daily,creatine phosphate sodium 1 g once daily,insulin injection (6 U + 5 ％ glucose injection 500 ml+potassium chloride injection 10 ml),Xueshuantong injection（血栓通注射液）500 mg once daily,mecobalamin injection 2 rnl once daily,and oral butylphthalide soft capsules 0.2 g thrice daily,ranitidine bismuth citrate capsules 0.2 g twice daily,etc.On day 7,the patient developed visual blurring and could not see distant objects clearly.At the same time,the patient experienced mydriasis with diameter about 5.0 mm and conjunctival congestion.Acyclovir was discontinued and other drugs were continued.Five days after withdrawal,his symptoms of conjunctival congestion,mydriasis,and vision were improved.At 2 months of follow-up,blurred vision did not recur.

A 24-year-old male patient with viral meningoencephalitis received intravenous infusion of acyclovir 0.5 g thrice daily,Xingnaojing injection（醒脑静注射液）20 ml once daily,mannitol injection 250 ml thrice daily,creatine phosphate sodium 1 g once daily,insulin injection (6 U + 5 ％ glucose injection 500 ml+potassium chloride injection 10 ml),Xueshuantong injection（血栓通注射液）500 mg once daily,mecobalamin injection 2 rnl once daily,and oral butylphthalide soft capsules 0.2 g thrice daily,ranitidine bismuth citrate capsules 0.2 g twice daily,etc.On day 7,the patient developed visual blurring and could not see distant objects clearly.At the same time,the patient experienced mydriasis with diameter about 5.0 mm and conjunctival congestion.Acyclovir was discontinued and other drugs were continued.Five days after withdrawal,his symptoms of conjunctival congestion,mydriasis,and vision were improved.At 2 months of follow-up,blurred vision did not recur.

Objective To investigate the value of CT guided interventional treatment in intracranial hematoma.Methods There were 108 cases with intracranial hematoma(30~100 ml,average of 50 ml) in our hospital from 2000~2003.Among them,58 cases were located in putamen,6 cases in subcortial,4 cases in thalamus,4 cases in cerebella,12 cases were traumatic eprdural hematoma and 12 cases were subdural hematoma.The table distance of the aspirated slice was the sum of table distance at the slice of the maximum hematoma and half slice thickness.It was considered successful for CT guided aspiration if the distance between the front of aspiration needle and the center ofthe biggest hematoma was not more than 1mm and it was regarded effective,when the patients' lives were saved and their life quality was improved.Results The success rate of CT guided aspiration was 99.8%.The effective rate of hypertensive intracerebralhemorrhage located in putamen,subcortial and thalamus was 94.3%.The effective rate of CT guided interventional treatment for cerebellar hypertensive intracerebral hemorrhage above 30ml was 0,the cure rate of traumatic epidural and subdural hematoma was 97.2%.Conclusion The effect of CT guided interventional treatment of intracranial hematoma is well except for cerebellar hematoma.