Mitophagy,as a highly conserved cellular defense mechanism,precisely recognizes and eliminates dys-functional or excessive mitochondria,thereby maintaining homeostasis of the mitochondrial network and providing a stable energy supply and raw materials for biosynthesis in cells.Mitophagy exerts regulatory effects on the develop-ment of osteosarcoma through multiple molecular mechanisms.Targeting this mechanism as a new strategy for thera-peutic intervention provides a solid theoretical foundation and set stage for exploring novel targeted therapies,there-by driving the field of osteosarcoma treatment towards more precise and efficient directions.