Although chromosomal mosaic embryos detected by trophectoderm(TE)biopsy offer healthy embryos available for transfer,high-resolution postnatal karyotyping and chromosome testing of the transferred embryos are insufficient.Here,we applied single-cell multi-omics sequenc-ing for seven infants with blastula chromosomal mosaicism detected by TE biopsy.The chromo-some ploidy was examined by single-cell genome analysis,with the cellular identity being identified by single-cell transcriptome analysis.A total of 1616 peripheral leukocytes from seven infants with embryonic chromosomal mosaicism and three control ones with euploid TE biopsy were analyzed.A small number of blood cells showed copy number alterations(CNAs)on seem-ingly random locations at a frequency of 0％-2.5％per infant.However,none of the cells showed CNAs that were the same as those of the corresponding TE biopsies.The blastula chromosomal mosaicism may be fully self-corrected,probably through the selective loss of the aneuploid cells dur-ing development,and the transferred embryos can be born as euploid infants without mosaic CNAs corresponding to the TE biopsies.The results provide a new reference for the evaluations of trans-ferring chromosomal mosaic embryos in certain situations.

Adenomyosis is a common disease in women of childbearing age, and is one of the important causes of infertility. Based on the relationship between adenomyosis and infertility, and the impact of adenomyosis on the outcome of assisted reproductive technology (ART), this article focused on the strategies of ART for patients with adenomyosis complicated with infertility, discussing the indications and timing of ART, pretreatment strategies, ovarian stimulation protocols and embryo transfer strategies.

Adenomyosis is a common disease in women of childbearing age, and is one of the important causes of infertility. Based on the relationship between adenomyosis and infertility, and the impact of adenomyosis on the outcome of assisted reproductive technology (ART), this article focused on the strategies of ART for patients with adenomyosis complicated with infertility, discussing the indications and timing of ART, pretreatment strategies, ovarian stimulation protocols and embryo transfer strategies.

As a member of galectins family, galectin-1(Gal-1)is widely expressed in tissues and cells, and participates in a variety of physiological and pathological processes, such as cell adhesion, proliferation, apoptosis and inflammatory reaction. Recently, it has been found that Gal-1 is highly expressed at the maternal-fetal interface and plays important roles in trophoblast cell proliferation, differentiation and invasion, endometrial receptivity, placental angiogenesis and maternal-fetal immune tolerance. In this review, we outline the expression of Gal-1 at the maternal-fetal interface and the involvement of Gal-1 in embryo implantation and pregnancy maintenance, to provide novel insights for the early diagnosis, prognostic assessment and treatment of early pregnancy loss and pregnancy-related diseases.