1.Effect of inhibiting LILRB2 on proliferation and apoptosis of human colorectal cancer SW480 cells
Hongwei PAN ; Jingjing WENG ; Yan ZHANG ; Zhizhi LIU ; Minya WANG ; Xiaofeng CHEN
Chinese Journal of Endocrine Surgery 2022;16(6):650-654
Objective:To explore the effect of LILRB2 on the proliferation and apoptosis of colorectal cancer SW480 cells, and to further explore its mechanism.Methods:Colorectal cancer SW480 cells were cultured in vitro and divided into blank control group, negative control group and experimental group. The expression of LILRB2 was detected by flow cytometry. The expression of LILRB2 was detected by qPCR, and the empty vector plasmid and the LILRB2 plasmid were transfected into SW480 cells respectively; cell proliferation was detected by CCK-8 method; cell apoptosis was detected by flow cytometry. Western blot was used to detect changes in the expression of related proteins.Results:The expression level of LILRB2 in SW480 was 0.84 ± 0.09, twice higher than that in FHC cells (0.38 ± 0.05) , and the difference was statistically significant ( P<0.05) . After virus infection, the expression of LILRB2 (0.48 ± 0.07) in SW480 cells of the experimental group decreased significantly. CCK-8 experiment results showed that after 12 hours of treatment, the proliferation of SW480 cells in the LILRB2 low expression experimental group was inhibited, and the percentage of apoptosis in SW480 cells in the LILRB2 low expression experimental group increased to 49.3%±1.2%, which was statistically significant ( P<0.05) compared with the percentage of apoptosis in the blank control group and the negative control group (7.48%±0.85%, 7.35%±0.93%) . The ROS level of SW480 cells in the experimental group with low LILRB2 expression was significantly higher than that in the blank control group and negative control group ( P<0.05) . After adding ROS scavenger NAC, the apoptosis of LILRB2 in the experimental group increased. Conclusion:The low expression of LILRB2 inhibits the proliferation of SW480 cells and induces apoptosis, which may play a role by regulating the level of ROS, providing a theoretical basis for the study of LILRB2 in colorectal cancer.
2.Application of Image Cloud Platform in Hierarchical Hospital Visit
Minya CHEN ; Weiping CHEN ; Ke LI
Chinese Journal of Medical Instrumentation 2017;41(3):228-230
Objective To achieve medical information sharing among different health institutes through constructing image cloud platform, thus establish a diagnosis and treatment system through long range image-viewing. Methods Design and apply image cloud platform in Wuxi people's hospital and other 16 basal level health institutes. Results The goal of sharing the medical equipment and imaging diagnosis expert collaboration at the regional medical institutions was achieved through establishment of the platform. Conclusion The establishment of such platform plays a positive role in balancing health resources, improves the level of basic-level hospital diagnosis and treatment and reduces medical cost.
3.Practice of information management platform for a medical alliance in Wuxi
Minya CHEN ; Weiping CHEN ; Chun LUO
Chinese Journal of Hospital Administration 2016;32(11):830-832
Covered in the paper is the practice of an information platform of a medical alliance centering on Wuxi People′s Hospital,and its application in 1 6 member hospitals.Thanks to the overall standardization,planning, construction, regulation and synergy of the members in tiered medical services,such a platform contributes positively in sharing management,technology and medical resources among the members.
4.The Intelligent Beds Monitoring System Based on Sensor Technology
Chinese Journal of Medical Instrumentation 2016;40(2):101-102
An inteligent beds monitoring system based on sensor technology is developed. Through the instalation of sensors in the bed, the patient’s vital signs like heart rate, breathing rate can be acquired, other information such as rol over, in/out of bed can be real-time monitored. Also, the system can automaticaly analysis data and remind the nurse if abnormal events happened. After one year's clinical application, the system received the affi rmation from patients and medical staff.
5.Construction of medical examination appointment platform in general hospital
Minya CHEN ; Chun LUO ; Ke LI
China Medical Equipment 2015;(1):118-120
Objective: To optimize the hospital medical examination process, reduce the patient waiting time. Methods: Established a centralized examination appointment center at outpatient department. Development and implementation of medical examination appointment platform, integrated with the hospital HIS system, PACS systems and queue system. Results:Achieved the function of“one-stop booking, network distribution, multi-point examination”for all patients, a better solution to the disorder problem of medication examination. Conclusion:The implementation of the medication examination platform has played a positive role. Facilitate patient visits, rational use of equipment, improve work efficiency and improve the treatment environment.
6.Implementation of ECG Monitoring System Based on Internet of Things.
Chinese Journal of Medical Instrumentation 2015;39(6):418-420
In order to expand the capabilities of hospital's traditional ECG device and enhance medical staff's work efficiency, an ECG monitoring system based on internet of things is introduced. The system can monitor ECG signals in real time and analyze data using ECG sensor, PDA, Web servers, which embeds C language, Android systems, .NET, wireless network and other technologies. After experiments, it can be showed that the system has high reliability and stability and can bring the convenience to medical staffs.
Electrocardiography
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Internet
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Monitoring, Physiologic
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Reproducibility of Results
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Wireless Technology
7.Implementation of ECG Monitoring System Based on Internet of Things
Chinese Journal of Medical Instrumentation 2015;(6):418-420
In order to expand the capabilities of hospital’s traditional ECG device and enhance medical staff’s work efficiency, an ECG monitoring system based on internet of things is introduced. The system can monitor ECG signals in real time and analyze data using ECG sensor, PDA, Web servers, which embeds C language, Android systems, .NET, wireless network and other technologies. After experiments, it can be showed that the system has high reliability and stability and can bring the convenience to medical staffs.
8.Research on design and application of medical waste management system
Minya CHEN ; Yi ZHANG ; Liangliang LU
China Medical Equipment 2014;(10):12-13,14
Objective: Medical waste management is not only the problem of hospital, but also is an important public health problem. Apply modern information technology to insure real-time tracking and monitoring medical waste recycling, storage, disposal, improve medical waste management. Methods: Construct medical waste management system with wireless network, bar code and RFID etc. Results:By this system, transportation, storage and disposal that process of medical waste within the hospital are monitoring and supervision effective. Conclusion:The collection and transportation sectors of medical waste have been standard, to ensure the security, enhanced transparency and improved hospital management.
9.Development and application of hospital customer service center platform.
Minya CHEN ; Konglin ZHENG ; Yong XIA
Chinese Journal of Medical Instrumentation 2012;36(1):77-78
This paper introduces the construction and application of the platform of client service center in the general hospital and discusses how to provide patients with an entire service including service before clinic, on clinic and after clinic. It can also provide references for a new service mode for clinic service.
Centralized Hospital Services
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methods
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organization & administration
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Hospital Administration
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methods
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Patient Satisfaction
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Patients
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Software Design
10.Practice and experience about construction of pharmacy automation at general hospital.
Minya CHEN ; Yong XIA ; Jiayi SHI
Chinese Journal of Medical Instrumentation 2011;35(2):155-156
This paper introduce how to combining the whole package automatic dispensing machine with intelligent storage cabinets at outpatient pharmacy. Furthermore, this paper introduce how to integrated this system with hospital information systems which can provide references for the construction of automatic hospital pharmacy in our country.
Automation
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methods
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Hospitals, General
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Medication Systems, Hospital
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Pharmacy Service, Hospital

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