[摘 要] 目的：探究新型α-位四取代磺化酞菁锌（ZnPcX6）与牛血清白蛋白（BSA）、人血清白蛋白（HSA）的相互作用，制备ZnPcX6-白蛋白复合物，并评估复合物对酞菁聚集状态的改善效果及其体外光动力抗肿瘤活性。方法：采用荧光光谱法、紫外-可见吸收光谱法检测ZnPcX6与BSA、HSA的相互作用并计算其结合常数与表观结合位点数；通过温育交换法制备ZnPcX6-白蛋白非共价复合物，利用凝胶排阻层析完成分离纯化并表征复合物光谱特征；采用MTT法评价ZnPcX6-BSA对HepG2肝癌细胞的体外光动力抗肿瘤活性，通过激光共聚焦显微镜观察HepG2细胞对ZnPcX6-白蛋白的摄取行为。结果：ZnPcX6与BSA的结合位点数为1.007，结合常数为8.41 × 105 L/mol；与HSA的结合作用更强，结合位点数为1.372，结合常数为1.14 × 108 L/mol，两种体系中ZnPcX6与白蛋白的摩尔结合比均约为1∶1。光谱分析显示，与游离ZnPcX6相比，ZnPcX6-白蛋白复合物中酞菁聚集体占比下降，单体占比提升，Q带最大吸收峰红移3 nm，复合物光谱性质得到改善。体外活性实验证实，ZnPcX6-BSA复合物对HepG2肝癌细胞的光动力抗肿瘤活性高于游离ZnPcX6；其IC50为0.52 μmol/L，细胞摄取实验表明与白蛋白形成复合物后，HepG2细胞对酞菁的摄取效率提高。结论：ZnPcX6可与BSA、HSA以约1∶1的摩尔比通过非共价结合形成复合物；与白蛋白复合后，ZnPcX6的聚集体占比下降；ZnPcX6-BSA可提高HepG2肝癌细胞对ZnPcX6的摄取，表现出更优的光动力抗肿瘤活性。

Objective:To explore the application effect of remazolam besylate in elderly hip fracture surgery and its impact on anesthesia recovery quality and bone metabolism indicators.Methods:A total of 100 elderly patients with hip fractures admitted to Ningde Hospital from January 2021 to April 2022 were selected, all of whom underwent hip replacement surgery. According to the random number table method, they were divided into an observation group ( n=50) and a control group ( n=50). We observed the use of drugs such as remifentanil besylate combined with sufentanil for anesthesia induction and remifentanil combined with other drugs for anesthesia maintenance; The control group received anesthesia induction with drugs such as midazolam combined with sufentanil, and anesthesia maintenance with drugs such as remifentanil. Comparisons were made between the analgesic effects of anesthesia, the quality of anesthesia recovery, and the levels of hemodynamic and bone metabolism indicators at different time points between the two groups. Results:The postoperative recovery time, eye opening time, and extubation time of the observation group were shorter than those of the control group (all P<0.05); The total incidence of restlessness and coughing was lower in the control group than in the control group ( P<0.05); There was no statistically significant difference in the levels of serum osteocalcin (BGP), parathyroid hormone (PTH), and bone alkaline phosphatase (BALP) between the two groups after treatment (all P>0.05); The heart rate (HR) and mean arterial pressure (MAP) of the observation group during skin cutting (T 2) and 5 minutes after extubation (T 3) were lower than those of the control group (all P<0.05), and SpO 2 was higher than that of the control group ( P<0.05). The excellent rate of anesthesia and analgesic effect in the observation group was significantly higher than that in the control group ( P<0.05). Conclusions:Remazolam besylate has a good clinical effect in hip surgery, and the quality of anesthesia recovery is relatively high, but it has little effect on the postoperative bone metabolism indicators.

Transportation service is an important part of hospital logistics management. In 2023, a tertiary hospital centered on patient service and guided by lean management, launched an intelligent transportation service in the " Didi" mode on the basis of the medical internet of things platform. This practice investigated the status of hospital transportation services and developed an intelligent transportation system that includes three subsystems: task reception and generation, dispatch and command of transportation center, and handheld computer accepting order. At the same time, standardized business processes were developed and transportation service performance evaluations were implemented. This practice had gradually piloted and achieved intelligent management of hospital transportation services. As of December 2023, the hospital had completed a total of about 310 thousand transportation tasks, of which 70% were intelligent system dispatching tasks. The timely delivery service rate had increased from 58% in 2022 to 98% in 2023. This practice had improved the quality and efficiency of transportation services, promoted the high-quality development of hospital logistics management, which could provide references for other hospitals to improve logistics management efficiency.

Red doctor’s culture is a precious spiritual wealth formed in the medical and health practice of the Central Soviet Area during a specific historical period. With the development of society and the changes of the times, the red doctor’s culture has been endowed with new connotation. Western Fujian was the earliest place to provide medical support for the revolutionary army, the birthplace of the Republic’s health service, and made great contributions to the victory of the Chinese revolution. The cultural resources of red doctor’s in western Fujian are abundant, and it is one of the important lineages of the red culture in western Fujian, with its unique historical value. At present, there are many problems in promoting the red doctor’s culture. Actively exploring and researching specific paths and methods to promote the red doctor’s culture is of great significance and value for giving full play to the connotation of the red doctor’s culture in the new era, promoting its value leading role at various levels of society, and passing down the spirit and culture of red doctor’s.

Trace metals deficiency or excess are associated with the etiology and pathogenesis of rheumatoid arthritis(RA). Aconiti Radix Cocta(A) and Paeoniae Radix Alba(B) are commonly used together for the treatment of RA. In this study, we aim to determine anti-arthritic-related metal bioavailability in the compatibility of herb A and B for avoiding metal deficiency or excess, and optimize the combination ratio of herb A and B, accordingly. Anti-arthritic-related metal bioaccessibility were evaluated by in vitro simulator of all gastrointestinal tract(including mouth, stomach, small and large intestines), and the roles of gastrointestinal digestive enzymes and intestinal microflora were investigated. Anti-arthritic-related metal bioavailability was assessed by the affinity adsorption with liposomes. The results indicated that compatibility proportion of corresponding herbal plants, gastrointestinal digestion and microbial metabolic, which could affect metal digestion and absorption. The optimal compatibility proportion of 1 A∶1 B is recommended, according to the dose of anti-arthritic-related metal bioavailability, which is often chosen for clinical practice of RA therapy. Thus, anti-arthritic-related metal bioavailability might be the key active substances for RA treatment.
Aconitum ; Biological Availability ; Drugs, Chinese Herbal ; Paeonia