To explore the relationship of neutrophil/lymphocyte ratio(NLR),25-hydroxyvitamin D3[25-(OH)D3]and periostin levels with immune function in children with Henoch-Sch?nlein purpura(HSP)and their prognostic value,120 children with HSP admitted to our hospital from January 2021 to June 2023 were selected as observation group,and 120 healthy children underwent physical examination during the same period were included as control group.Corresponding methods were used to detect the levels of NLR,serum 25-(OH)D3,periostin and peripheral blood lymphocyte subsets(CD3+,CD4+,CD8+,CD19+,CD56+),IgA,IgM,IgG,C3 and C4,and Spearman correlation analysis was used to analyze the correlation among them.After discharge,the children were followed up for 6 months to observe the prognosis and divided into favorable prognosis group and poor prognosis group,and the levels of NLR,25-(OH)D3 and periostin in the two groups were analyzed and compared.The risk factors for poor prognosis in HSP children were analyzed by logistic regression,and ROC curve was drawn to analyze the predictive value of the three indicators for prognosis.Compared with the control group,the NLR and periostin level in observation group were significantly increased,the 25-(OH)D3 level was significantly decreased(t value ranged from 10.604 to 19.332,P＜0.05),the levels of lymphocyte subsets CD3+,CD4+,CD4+/CD8+,CD19+and CD56+were decreased,the levels of CD8+were increased(t value ranged from 9.368 to 23.587,P＜0.05),the levels of IgA and IgG in peripheral blood were increased,while the levels of IgM,C3 and C4 were significantly decreased(t value ranged from 6.376 to 26.048,P＜0.05).Compared with the HSP children in the good prognosis group,poor prognosis group demonstrated significant differences in disease duration,respiratory tract infection,the levels of white blood cells,NLR,25-(OH)D3 and periostin(t values ranged from 3.006 to 10.806,χ2=5.133,P＜0.05),and all were risk factors affecting the prognosis.The AUC value,sensitivity and specificity of NLR,25-(OH)D3 and periostin combination for predicting the prognosis of HSP children were 0.937,91.86%and 76.47%,respectively,which were higher than the prediction efficacy of the above indexes alone(P＜0.05).In conclusion,NLR,25-(OH)D3 and periostin are related to immune function in children with HSP,and NLR,25-(OH)D3 and periostin are independent influencing factors of HSP poor prognosis,thus combination detection of the three indicators has certain predictive value for prognosis of HSP.

To explore the relationship of neutrophil/lymphocyte ratio(NLR),25-hydroxyvitamin D3[25-(OH)D3]and periostin levels with immune function in children with Henoch-Sch?nlein purpura(HSP)and their prognostic value,120 children with HSP admitted to our hospital from January 2021 to June 2023 were selected as observation group,and 120 healthy children underwent physical examination during the same period were included as control group.Corresponding methods were used to detect the levels of NLR,serum 25-(OH)D3,periostin and peripheral blood lymphocyte subsets(CD3+,CD4+,CD8+,CD19+,CD56+),IgA,IgM,IgG,C3 and C4,and Spearman correlation analysis was used to analyze the correlation among them.After discharge,the children were followed up for 6 months to observe the prognosis and divided into favorable prognosis group and poor prognosis group,and the levels of NLR,25-(OH)D3 and periostin in the two groups were analyzed and compared.The risk factors for poor prognosis in HSP children were analyzed by logistic regression,and ROC curve was drawn to analyze the predictive value of the three indicators for prognosis.Compared with the control group,the NLR and periostin level in observation group were significantly increased,the 25-(OH)D3 level was significantly decreased(t value ranged from 10.604 to 19.332,P＜0.05),the levels of lymphocyte subsets CD3+,CD4+,CD4+/CD8+,CD19+and CD56+were decreased,the levels of CD8+were increased(t value ranged from 9.368 to 23.587,P＜0.05),the levels of IgA and IgG in peripheral blood were increased,while the levels of IgM,C3 and C4 were significantly decreased(t value ranged from 6.376 to 26.048,P＜0.05).Compared with the HSP children in the good prognosis group,poor prognosis group demonstrated significant differences in disease duration,respiratory tract infection,the levels of white blood cells,NLR,25-(OH)D3 and periostin(t values ranged from 3.006 to 10.806,χ2=5.133,P＜0.05),and all were risk factors affecting the prognosis.The AUC value,sensitivity and specificity of NLR,25-(OH)D3 and periostin combination for predicting the prognosis of HSP children were 0.937,91.86%and 76.47%,respectively,which were higher than the prediction efficacy of the above indexes alone(P＜0.05).In conclusion,NLR,25-(OH)D3 and periostin are related to immune function in children with HSP,and NLR,25-(OH)D3 and periostin are independent influencing factors of HSP poor prognosis,thus combination detection of the three indicators has certain predictive value for prognosis of HSP.

Objective: To explore the effects of down-regulated ITGB5 expression on the proliferation of keloid fibroblasts and clarify the possible role of β5-integrin(ITGB5) in keloid. Methods: Construct lentiviral sh-RNA-expression vector targeting ITGB5 and infect keloid fibroblasts, the expression of ITGB5 were detected by Western Blot, the proliferation ability was identified by MTT. Results: The expression quantity of ITGB5 mRNA and protein in KFb group, LV-NC group and LV-KFb group are 1.00±0.00, 1.08±0.05, 0.34±0.01 and 0.91±0.03, 0.93±0.02, 0.28±0.07. Compared with LV-NC group and KFb group, the expression quantity of ITGB5 mRNA and protein in LV-KFb group decreased significantly(P<0.01). Compared with LV-NC group and KFb group, the proliferation rate decreased significantly in LV-KFb group at 48 h(P<0.01). Conclusions These results suggest that ITGB5 can accelerate fibroblasts proliferation in keloids.

Objective To study the clinical and genetic characteristics of keloid through investigating on four Han Chinese pedigrees.Methods The pedigree information and clinical data from Han Chinese keloid pedigrees were collected,which consisted of 22 patients in 127 family members,and then the charts of these pedigrees were constructed according to the data.Using the genetic model and pedigree analyses we summarized the clinical features of the disease in the families.Results Four Han Chinese keloid pedigrees were discovered.The three pedigree spans included 3 generations and one was 4 generations.Incidence of KD in the consanguinity family member was 23.7％ (23/93),and 20.8％ (11/53) in male KD,and 27.5％ (11/40) in female.Incidence of anterior chest KD was 40.9 ％.The inheritance pattern observed in these pedigrees was consistent with an autosomal dominant inheritance multi-gene hereditary disease with incomplete penetrance,and its nonpenetrance of KD gene carriers was 12％ (3/25).Conclusions The pattern of inheritance observed in these four Han Chinese keloid pedigrees is similar to previous reports and no gender differences are found in the incidence of disease,but differences in pathogenic site.Pedigree investigation helps to reveal the genetic characteristics of keloid.

OBJECTIVETo investigate the genome structure variation (SV) related with keloid using the whole-gene resequencing technology.

METHODSWe studied a keloid pedigree containing 4 generation of 27 people. 5 people (4 cases of keloid patients, and 1 case of normal) were selected to extract the genomic DNA. Then the whole-gene resequencing technique was used to check the variations.

RESULTSThrough database comparison and variation annotation analysis, we obtained 2 SVs associated with keloid formation. We used DAVID software to do the gene ontology and pathway analysis. We found a 168 bp inversion in gene tetraspanin 8 (TSPAN8) in all keloid patients, which contained the forth exon of TSPAN8.

CONCLUSIONSThere was no report about SVs related to keloid. In this study, we found 2 SVs associated with keloid, especially TSPAN8. The tumor cells express the TSPAN8 can up-regulate the vascular endothelial growth factor and its receptors, promote the adjacent fibroblasts secrete matrix metalloproteinases and uridylyl phosphate adenosine. So we hypothesis that the inversion of the forth exon in TSPAN8 may lead to the signal transduction disorder in the keloid patients. This study was a preliminary research. It needs a further study containing large sample to confirm.

Base Sequence ; Female ; Humans ; Keloid ; genetics ; Male ; Molecular Sequence Data ; Pedigree ; Sequence Analysis ; methods ; Tetraspanins ; genetics

OBJECTIVETo investigate the effects of botulinum toxin type A (BTXA) on the expression of alpha smooth muscle actin(alpha-SMA) and myosin-II of fibroblasts in scars. Methods Fibroblasts were isolated from tissue specimens of scars contracture. Cells from passages 3-5 were randomly divided into 3 groups (control group, low BTXA group (1 U/10(6) Cells), and high BTXA group (2.5 U/ 10(6)Cells)). Growth condition of fibroblasts was observed at 1 , 4, 7 day after BTXA treated. Changes of alpha-SMA and myosin-II in fibroblasts were detected by Western blot.

RESULTSFibroblasts grew well in control group. The proliferation was decreased 4 days later in BTXA groups. Lots of apoptotic cells were seen in high BTXA group at 7th day. Proteins of alpha-SMA and myosin-II in fibroblasts were statistically different between BTXA group and control groups at 4th day (P < 0.05). The expression of alpha-SMA and myosin-II in low BTXA group was higher than that in high BTXA group at 7th day (P < 0.05).

CONCLUSIONSBTXA could induce the apoptosis of fibroblasts and decrease the expression of alpha-SMA and myosin-II in fibroblasts. The inhibitory effect was strengthened with BTXA concentration increase within a certain range.

Actins ; metabolism ; Botulinum Toxins, Type A ; pharmacology ; Cicatrix ; Fibroblasts ; drug effects ; metabolism ; Humans ; Muscle, Smooth ; metabolism ; Myosin Type II ; metabolism ; Random Allocation

OBJECTIVETo investigate the genome copy number variation (CNV) related with keloid using the whole-gene resequencing technology.

METHODSA keloid pedigree containing 4 generation of 27 people was studied. Five people (4 cases of keloid patients, and 1 case of normal) were selected to extract the genomic DNA. Then the whole-gene resequencing technique was used to check the variations based on the Illumina Hiseq 2000.

RESULTSThrough database comparison and variation annotation analysis, 15 CNVs associated with scar hyperplasia were obtained. DAVID software was used to do the Gene Ontology and pathway analysis. Five CNVs were closely related to the keloid formation. They were growth factor receptor-bound 7 (Grb7), mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), mitogen-activated protein kinase kinase kinase 15 (MAP3K15), kruppel-like factors 7 (KLF7) and NK2 homeobox 2 (NKX2-2). These CNVs were involved in the process of epidermal cells formation and differentiation, cell exocrine and cell adhesion.

CONCLUSIONSThere are 5 CNVs associated with scar hyperplasia. Especially MAP3K15 and MAP4K4 deserve more research to find their function in keloid formation.

Cicatrix ; genetics ; DNA Copy Number Variations ; Female ; Humans ; Male ; Pedigree

Objective To evaluate the validity of botulinum toxin type A (BTXA) injections for the treatment of scar contracture.Methods 26 patients with scar contracture were randomly assigned into BTXA group and triamcinolone acetonide (TAC) group.Pinpoint tattooing was performed on each side of each scar in the plane of its longest axis.A template was used to ensure consistent length.These two tattoo points were measured to assess scar contraction at baseline,at every month for a total of 6 months.Histological analysis was conducted to study the physiological environment and immunohistochemistry to detect the expression of α-SMA and myosin-Ⅱ at different groups.Results Scar contraction was more relaxed in BTXA group than that in TAC group after 1 month (P＜0.05),especially in the 6th month (the D value in BTXA group and TAC group was (1.23±0.42) cm,and (0.56±0.33) cm respectively).For immunohistochemistry,the expression of α-SMA and myosin-Ⅱ also decreased in BTXA group (P＜0.05).Conclusions The treatment of scar contracture by suitable BTXA injections is safe and effective.

Objective:To investigate the relation of antiradiation effect of LJP and lymphocyte apoptosis. Methods:36 Wistar rats were randomly divided into 6 groups (n=6): control, model,and LJP given i.g.at 4 doses(100,200,300,400 mg/ kg bw) for 10d before whole-body irradiation with 9.0 Gy Co?-ray. 18h later,the effects of LJP on the indices of immune function of the irradiated rats 60 were measured. TUNEL and flow cytometry were used to study the effects of LJP on splenic lymphocyte apoptosis and immunohistochemical technique was used to detect the expression of Bcl-2 and the Bax protein. Results:LJP significantly modulated immune function in irradiated rats. The apoptosis ratio of splenic lymphocyte of the model group was higher than those of other groups. LJP could markedly inhibit the effects of irradiation on apoptosis and increase the ratio of bcl-2/bax protein in dose-effect manner. Conclusion:LJP could inhibit splenic lymphocyte apoptosis induced by irradiation, and its mechanism is associated with regulating the expression of bcl-2 and bax protein of splenic lymphocyte. Key word:laminaria japonica polysaccharides; irradiation; lymphocyte; apoptosis apoptosis-related genes