Objective: To analyze the serological identification methods of the coexisting anti-c alloantibodies and anti-C, and anti-e mimicking autoantibodies in an infant with autoimmune hemolytic anemia (AIHA), and to explore the strategies for precisely differentiating the two types of antibodies and guiding safe blood transfusion. Methods: Using the saline tube method and the microcolumn agglutination anti-human globulin method, blood type identification was carried out on a specimen from one patient with difficult crossmatching, including ABO, Rh (D), MNS, Duffy, Diego, and Lewis blood type identification, antibody screening, antibody specificity identification, direct anti-human globulin test and crossmatching test；multiple absorption-elution test, dilution method and major crossmatching with red blood cells of specific antigens for further antibody confirmation；and the simple capillary ultracentrifugation method to separate new and old red blood cells, followed by red blood cell Rh system blood type antigen identification. Results: Serological testing showed the child had blood type A, Rh type CCDee, with auto-positive reactions, positive direct antiglobulin and antibody screen tests. Through multiple adsorption-elution tests, dilution tests, and major crossmatching with red cells of specific antigens, the child's serum was found to contain anti-c alloantibodies and anti-C and anti-e mimicking autoantibodies. The patient was transfused with the least incompatible A-type CCDee red blood cells, and the transfusion was effective. Conclusion: In the diagnosis and treatment of AIHA in infants, it is crucial to be highly vigilant about the coexistence of alloantibodies and mimicking autoantibodies. It is necessary to rely on standardized serological tests to accurately distinguish them. For children with combined anti-c alloantibodies and anti-C and anti-e mimicking autoantibodies, blood transfusion should preferably choose blood products with negative c antigen and the same Rh type to ensure blood transfusion safety and long-term tolerance.

Objective: To explore the application of high hydrostatic pressure in the development of melanoma vaccine.Methods:The high hydrostatic pressure,liquid nitrogen freezing and thawing were used to break the murine B16 melanoma cells and then the cell suspension was mixed with Freund's adjuvant to prepare vaccine for immunizing the mice.Results:After immu-nization,the murine B16 melanoma cells were injected intravenously and subcutaneously.The immune results of the vaccines were evaluated,by comparing survival time of the mice, subcutaneous tumor volume, DTH experiments and the fluorescence imaging of tumors.Conclusion:Compared with the method of liquid nitrogen freeze-thaw broken,high hydrostatic pressure crushing cells has more advantages in the development of tumor vaccine.