Objective: To explore the expression of collagen type 1 alpha 2 (COL1A2) in cervical cancer and its correlation with immune infiltration. Methods: Bioinformatics techniques were used to analyze the expression of COL1A2 in cervical cancer. Western blot and RT-qPCR were used to detect the expression of COL1A2 in cervical cancer tissues and cell lines. The correlation between the expression of COL1A2 and tumor immune cell infiltration was analyzed by tumor immune estimation resource (TIMER2. 0) . Gene set enrichment analysis (GSEA) was used to analyze the possible mechanism of COL1A2 in cervical cancer. Jaspar database was used to predict the transcrip- tion factors of COL1A2. Western blot and RT-qPCR were used to detect the expression of transcription factors in cervical cancer tissues and cell lines. Results: The expression of COL1A2 was down-regulated in cervical cancer (P < 0. 05) . The expression of COL1A2 was positively correlated with the levels of macrophages and myeloid den- dritic cells (P < 0. 01) . The proportions of 22 types of immune cells were different in different cervical cancer pa- tients. In addition , compared with the high expression group of COL1A2 , the proportion of M0 macrophages , M2 macrophages and resting memory CD4 + T cells increased in the low expression group of COL1A2 , while the propor- tion of CD8 + T cells , activated memory CD4 + T cells , follicular helper T cells , activated NK cells and activated myeloid dendritic cells decreased (P < 0. 05) . GSEA analysis showed that COL1A2 was related to immune-related signaling pathways , including Notch signaling pathway , interleukin-6/janus kinase/signal transducer and activator of transcription 3 (IL6/JAK/STAT3) , Wnt/β-catenin signaling pathway , etc. (P < 0. 01) . Jaspar database pre- dicted that the transcription factor of COL1A2 was paired box protein 5 (PAX5) , and the expression of PAX5 de- creased in cervical cancer (P < 0. 05) . Conclusion COL1A2 is expected to become a potential diagnostic biomar- ker and immunotherapy target for cervical cancer.

Objective : To investigate the spatial distribution and the influencing factors of hypertensive patients aged 45 years and above,and to provide evidence for the prevention and control of hypertension among middle-aged and elderly population. Methods : Multi-stage stratified random sampling method was used to collect 150 counties（cities or districts）from 30 provinces（municipalities or autonomous regions）from CHARLS database in 2015 according to the per capita GDP in different areas. Then the probability proportionate to size sampling method was used to select three villages（neighborhood committees）,80 families were randomly selected from each village（neighborhood committees）,and residents aged 45 years and above were interviewed. Spatial analysis was based on the vector map of China's provincial boundaries,Geoda and ArcGIS 10.2 software were used to analyze the prevalence and influencing factors of hypertension at provincial levels. Results : The health data of 14 880 people aged 45 years and above in 28 provinces（municipalities or autonomous regions）were obtained,including 4 203 patients with hypertension（28.25%）and 5 408 people with pre-hypertension（36.34%）. The results of local spatial analysis showed that the clustering regions of hypertension patients were in northeast and eastern parts of China. The results of spatial regression analysis showed that males,age of 80 years and above and overweight were the main risk factors for hypertension（all P<0.05）,which had greater influence on the middle part of China,north and northeast of China,and the east and north of China,respectively. Conclusion Males,age of 80 years and above and overweight were the main risk factors for hypertension in middle-aged and elderly population in China. The main prevention and control areas of hypertension were eastern and northeast China.

Objective:To evaluate the impact of atorvastatin and rosuvastatin at different doses on liver function in the patients with coronary artery disease. Methods:A retrospective study was used, and the patients with coronary heart disease hospitalized in the first hospital of China medical university from January 2013 to March 2014 were selected. According to the drug variety and dosage, the patients were divided into four groups: A (atorvastatin, 20 mg·d-1), B (atorvastatin, 40 mg·d-1), C (rosuvastatin, 10 mg· d-1 ) and D ( rosuvastatin, 20 mg·d-1 ) . The basic information of patients, the indices of liver function( ALT,AST,ALP and TBIL) , the time of abnormal liver function, treatment strategies and prognosis were recorded. The incidence of abnormal liver function in the groups was compared, and the rationality and effectiveness of the intervetion strategies were evaluated. Results:A total of 269 patients were collected. Among them, 149 cases were with abnormal liver function in varying degrees, and 21 patients were with ALT>3?ULN. The incidence of ALT>3?ULN in atorvastatin high dose group was much higher than that in atorvastatin low dose group( P<0. 05). Conclusion:The patients treated with high dose atorvastatin (40 mg·d-1) have a high risk of liver injury.

Objective To observe HA14-1 sensitizes Lewis lung carcinoma in mice to cyclophosphamide (CTX) and to explore its possible mechanism. Methods Forty Lewis lung carcinoma model mice were randomly divided into 4 groups:normal saline group,CTX group, HA14-1 group,CTX+HA14-1 group. After the treatment of 7 days,all of the mice were killed on the 22nd day of tumor inoculation. The tumor volume growth curve of each group was described;tumor inhibition rate was caculatued;Bcl-2,Bax,Caspase-9 protein expression levels before and after the treatment were determined by immunohistnehemistry. Results Compared to the normal saline group,HA14-1 group had no significant effect on inhibiting tumor volume,and the tumor volume in HA14-1 group increased less slowly than that of CTX group, HA14-1 group and CTX+HA14-1 group. Compared to the normal saline group, the tumor inhibition rate of HA14-1 group had no significant increase (P> 0.05),while that of CTX group and CTX + HA14-1 group increased significantly (P< 0.05);compared to the CTX group,the tumor inhibition rate of CTX + HA14-1 group increased significantly (P < 0.05). Bcl-2 protein expression levels in CTX group,HA14-1 group and CTX+HA14-1 group were lower than that in the normal saline groups compared to CTX group,Bcl-2 protein expression of CTX + HA14-1 group reduced significantly. Compared to the normal saline group,the expression levels of Bax and Caspase-9 protein in CTX group, HA14-1 group and CTX+HA14-1 group increased significantly (P< 0.05);compared to CTX group,CTX + HA14-1 group increased more significantly (P < 0.05). Conclusion HA14-1 might enhance the efficiency of CTX chemotherapy via inhibiting the expression of Bcl-2, increasing the expression of Bax and caspase-9 and promoting tumor cell apoptosis.

OBJECTIVE: To review and explore the clinical characteristics, acoustic features, early diagnosis and prognosis of epidemic parotitis being accompanied by acute pancreatitis and sensorineural hearing loss (SNHL). METHOD: Report and analysis the clinical data of a patient with epidemic parotitis being accompanied by acute pancreatitis and sensorineural hearing loss and review the relevant literatures. RESULT: Epidemic parotitis virus could produce lesion in more than one organ resulting in the corresponding complications. It was uncommon when a patient presented epidemic parotitis, acute pancreatitis and sensorineural hearing loss at the same time. Unilaterally total hearing loss was the most common type of the SNHL, and the prognosis was bad. CONCLUSION Epidemic parotitis can be accompanied by one or more complication, including SNHL. Auditory brainstem response (ABR) is of great value in early diagnosis of SNHL in patients with epidemic parotitis.
Child ; Female ; Hearing Loss, Sensorineural ; complications ; Humans ; Mumps ; complications ; Pancreatitis ; complications

According to the syndrome differentiation, 50 cases of cervical spondylopathy were treated by puncturing the distal acupoints and performing massage manipulation on the local affected area. After 2 courses of treatment, 35 cases were cured, 8 cases were markedly effective, 6 cases were improved,1 case was ineffective.