ObjectiveTo explore the mechanisms of Qingchang Wenzhong Decoction （清肠温中方， QWD） in treating ulcerative colitis （UC）. MethodsA total of 100 male C57BL/6J mice were assigned to a pseudo-germ-free group （n=45） and a conventional group （n=55） using a random number table. Pseudo-germ-free model was established by giving antibiotic treatment and subsequently subjected to acute UC induction. After the model was successfully established， the 45 pseudo-germ-free acute UC mice were randomly allocated to a pseudo-germ-free model group， a pseudo-germ-free QWD group， and a pseudo-germ-free mesalazine group， with 15 mice in each group. The 55 conventional mice were randomly divided into a normal control group （n=10）， as well as a conventional model group， a conventional QWD group， and a conventional mesalazine group， with 15 mice in each group. Except for the normal control group， acute UC models were established in all other groups. Mice in the control group received no intervention. From day 32 of the experiment， mice in the pseudo-germ-free QWD group and the conventional QWD group were administered with QWD granules at a dose of 9.71 g/（kg·d） by gavage with deionized water， while those in the pseudo-germ-free mesalazine group and the conventional mesalazine group were given mesalazine enteric-coated tablets at a dose of 151.67 mg/（kg·d） by gavage. Mice in the remaining groups received an equal volume of deionized water once daily for 7 consecutive days. On day 25 of the experiment， six mice were randomly selected from both the pseudo-germ-free group and the conventional group to measure body weight， and fecal samples were collected for 16S rDNA sequencing to compare differences in gut microbiota， including operational taxonomic units （OTUs）， Shannon index， Observed_species index， and phylogenetic diversity （PD whole tree）. On day 39， body weight was measured in all groups， and the disease activity index （DAI） was evaluated. Colon length and colon wet weight were recorded. Histopathological changes in colonic tissues were assessed by hematoxylin and eosin （HE） staining， and histological injury scores were determined. In addition， the expression levels of mucopolysaccharides and mucin 2 （MUC2） in colonic tissues were measured. ResultsOn day 25， there was no significant difference in body weight between the pseudo-germ-free group and the conventional microbiota group （P＞0.05）. Compared to the conventional microbiota group， the pseudo-germ-free group showed significantly reduced OTUs， Shannon index， Observed_species index， and PD whole tree （P＜0.01）. On day 39， compared to the normal control group， mice in the conventional model group showed a significant decrease in the body weight and an increase in DAI， along with increased colon wet weight， shortened colon length， markedly elevated histopathological scores， and reduced expression of mucopolysaccharides and MUC2 （P＜0.01）. In contrast， no significant differences were observed in these parameters in the pseudo-germ-free model group （P＞0.05）. Compared to the conventional model group， the conventional QWD group showed significant improvement in all measured parameters， whereas the conventional mesalazine group showed reductions only in DAI scores and colon wet weight （P＜0.01）. No statistically significant differences were observed among the pseudo-germ-free model group， the pseudo-germ-free QWD group， and the pseudo-germ-free mesalazine group （P＞0.05）. ConclusionQWD may promote the reconstruction of the intestinal mucus barrier by regulating the intestinal flora， thereby exerting a therapeutic effect on acute UC.

ObjectiveTo explore the mechanisms of Qingchang Wenzhong Decoction （清肠温中方， QWD） in treating ulcerative colitis （UC）. MethodsA total of 100 male C57BL/6J mice were assigned to a pseudo-germ-free group （n=45） and a conventional group （n=55） using a random number table. Pseudo-germ-free model was established by giving antibiotic treatment and subsequently subjected to acute UC induction. After the model was successfully established， the 45 pseudo-germ-free acute UC mice were randomly allocated to a pseudo-germ-free model group， a pseudo-germ-free QWD group， and a pseudo-germ-free mesalazine group， with 15 mice in each group. The 55 conventional mice were randomly divided into a normal control group （n=10）， as well as a conventional model group， a conventional QWD group， and a conventional mesalazine group， with 15 mice in each group. Except for the normal control group， acute UC models were established in all other groups. Mice in the control group received no intervention. From day 32 of the experiment， mice in the pseudo-germ-free QWD group and the conventional QWD group were administered with QWD granules at a dose of 9.71 g/（kg·d） by gavage with deionized water， while those in the pseudo-germ-free mesalazine group and the conventional mesalazine group were given mesalazine enteric-coated tablets at a dose of 151.67 mg/（kg·d） by gavage. Mice in the remaining groups received an equal volume of deionized water once daily for 7 consecutive days. On day 25 of the experiment， six mice were randomly selected from both the pseudo-germ-free group and the conventional group to measure body weight， and fecal samples were collected for 16S rDNA sequencing to compare differences in gut microbiota， including operational taxonomic units （OTUs）， Shannon index， Observed_species index， and phylogenetic diversity （PD whole tree）. On day 39， body weight was measured in all groups， and the disease activity index （DAI） was evaluated. Colon length and colon wet weight were recorded. Histopathological changes in colonic tissues were assessed by hematoxylin and eosin （HE） staining， and histological injury scores were determined. In addition， the expression levels of mucopolysaccharides and mucin 2 （MUC2） in colonic tissues were measured. ResultsOn day 25， there was no significant difference in body weight between the pseudo-germ-free group and the conventional microbiota group （P＞0.05）. Compared to the conventional microbiota group， the pseudo-germ-free group showed significantly reduced OTUs， Shannon index， Observed_species index， and PD whole tree （P＜0.01）. On day 39， compared to the normal control group， mice in the conventional model group showed a significant decrease in the body weight and an increase in DAI， along with increased colon wet weight， shortened colon length， markedly elevated histopathological scores， and reduced expression of mucopolysaccharides and MUC2 （P＜0.01）. In contrast， no significant differences were observed in these parameters in the pseudo-germ-free model group （P＞0.05）. Compared to the conventional model group， the conventional QWD group showed significant improvement in all measured parameters， whereas the conventional mesalazine group showed reductions only in DAI scores and colon wet weight （P＜0.01）. No statistically significant differences were observed among the pseudo-germ-free model group， the pseudo-germ-free QWD group， and the pseudo-germ-free mesalazine group （P＞0.05）. ConclusionQWD may promote the reconstruction of the intestinal mucus barrier by regulating the intestinal flora， thereby exerting a therapeutic effect on acute UC.

Real-time acquisition of pulmonary ventilation and perfusion information through thoracic electrical impedance tomography (EIT) holds significant clinical value. This study proposes a novel method based on the rime (RIME) algorithm-optimized variational mode decomposition (VMD) to separate lung ventilation and perfusion signals directly from raw voltage data prior to EIT image reconstruction, enabling independent imaging of both parameters. To validate this approach, EIT data were collected from 16 healthy volunteers under normal breathing and inspiratory breath-holding conditions. The RIME algorithm was employed to optimize VMD parameters by minimizing envelope entropy as the fitness function. The optimized VMD was then applied to separate raw data across all measurement channels in EIT, with spectral analysis identifying relevant components to reconstruct ventilation and perfusion signals. Results demonstrated that the structural similarity index (SSIM) between perfusion images derived from normal breathing and breath-holding states averaged approximately 84% across all 16 subjects, significantly outperforming traditional frequency-domain filtering methods in perfusion imaging accuracy. This method offers a promising technical advancement for real-time monitoring of pulmonary ventilation and perfusion, holding significant value for advancing the clinical application of EIT in the diagnosis and treatment of respiratory diseases.
Humans ; Electric Impedance ; Algorithms ; Tomography/methods* ; Pulmonary Ventilation/physiology* ; Lung/diagnostic imaging* ; Image Processing, Computer-Assisted/methods* ; Adult

Objective This study aims to investigate the expression of YTH domain N6-methyladenine RNA binding protein 2(YTHDF2)and UBX domain protein 1(UBXN1)in glioma tissue and their prognostic value.Methods A total of 92 glioma cases that underwent surgical treatment in Qingdao Jiaozhou Central Hospital from February 2017 to February 2018 were included.Immunohistochemistry was used to detect YTHDF2 and UBXN1 expression.Spearman rank correlation analysis was conducted.Kaplan-Meier survival curves were plotted to analyze the association between YTHDF2,UBXN1 expression and prognosis in glioma patients.COX analysis was used to determine the prognostic factors affecting glioma patients.Results Compared with adjacent tissues,the positivity rate of YTHDF2(65.22％vs 15.22％)was significantly higher in gliomas,while the positivity rate of UBXN1(26.09％vs 73.91％)was lower,and differences were statistically significant(x2=47.831,42.087,all P<0.05).Spearman rank correlation analysis,showed a negative correlation between YTHDF2 and UBXN1 expression in gliomas(r=-0.712,P<0.05).Compared with tumors diameter<3cm and WHO grades Ⅰ to Ⅱ,YTHDF2(75.47％vs 51.28％,65.22％vs 50.00％)had a higher positivity rate in glioma tissues with tumor diameter≥3cm and WHO grade Ⅲ,while UBXN1(15.09％vs 41.03％,11.11％vs 47.37％)had a lower positivity rate,and differences were statistically significant(x2=5.795,6.609;7.835,15.207,all P<0.05).The five-year overall survival rate of YTHDF2 positive group was lower than that of negative group[28.33％(17/60)vs 62.50％(20/32)],while the five-year overall survival rate of UBXN1 positive group was higher than that of negative group[66.67％(16/24)vs 30.88％(21/68)],and the differences were statistically significant(Log-Rank x2=12.870,7.665,all P<0.05).YTHDF2 positive(HR=2.427,95％CI:1.426～4.569),UBXN1 negative(HR=1.740,95％CI:1.121～2.568),WHO grade Ⅲ(HR=2.671,95％CI:1.160～6.012)and tumor diameter≥3cm(HR=1.628,95％CI:1.017～2.592)were risk factors for poor survival prognosis in glioma patients.Conclusion YTHDF2 increased and UBXN1 decreased in glioma tissues,both of which are related to WHO grading and tumor diameter,and they are independent factors for evaluating the prognosis of glioma patients.

Objective To explore the effect of hospital health management mode in patients with prediabetes.Methods Eighty pre-diabetes patients who were randomly chosen from physical examination people in the physical examination center of our hospital from April 2011 to June 2011 were divided into the observation group and the control group.The observation group received the hospital health management mode of pre diabetes designed by ourselves,and the control group received the traditional management mode.The awareness rate of diabetes in patients with pre-diabetes,the fasting plasma glucose (FPG),2 h postprandial plasma glucose (2 h PG),systolic blood pressure (SBP),diastolic blood pressure (DBP),low-density lipoprotein (LDL),body mass index (BMI) were evaluated at the beginning of the study,six month after the study and one year after the study.The conversion rate of diabetes was compared one year after the study.Results The awareness rate of diabetes in patients with pre-diabetes,the levels of FPG,2 h PG,SBP and DBP were respectively(5.68 ± 0.43) mmol/L,(9.63 ± 0.67) mmol/L,(117.40 ± 7.40) mmHg,(72.90 ± 5.10) mmHg in the observation group,and were (6.84 ± 1.32) mmol/L,(10.75 ±0.91) mmol/L,(125.60 ± 10.20) mmHg,(77.30 ±6.91) mmHg in the control group,and the differences were statistically significant (x2 =19.156,t =5.030 6,2.285 2,3.955 4,2.615 2,respectively; P ＜ 0.05).The levels of LDL and BMI in the observation group showed a downward trend,but no differences were found in two groups (P ＞ 0.05).One year after the study,the levels of FPG,2 h PG,SBP,DBP,LDL and BMI in the observation group were respectively (5.57 ± 0.38) mmol/L,(9.51 ±0.47) mmol/L,(116.23±7.93) mmHg,(70.4±4.3) mmHg,(3.00±0.37) mmol/L and (23.4 ±2.48)kg/m2,and were lower than (6.91 ±1.15) mmol/L,(10.86±3.21) mmol/L,(128.3± 12.14) mmHg,(79.2 ±9.56) mmHg,(3.6 ±0.41) mmol/L and (27 ±5.41) kg/m2 in the control group,and the differences were statistically significant (P ＜0.05) ; the conversion rate of diabetes was 2.7％ (1/37)in the observation group,and was lower than the 15.8％ (6/38) in the control group,and the difference was statistically significant (x2 =2.405 1,P ＜ 0.05).Conclusions The hospital health management mode of pre diabetes designed by ourselves can improve the awareness rate of diabetes in patients with pre-diabetes,decrease the level of FPG,2hPG,SBP,DBP,LDL and BMI,and reduce the conversion rate of diabetes.

Objective To evaluate the frequencies and polymorphisms of CCR5-△32,CCR2-641 and SDF1-3'A alleles conferring resistance to HIV-1 infection in Chinese population from Han ethnic origin.Methods This cohort was comprised of 1251 subjects(915 men and 336 women)aged 15 -80 years and none was HIV-1 positive.Genotyping of allelic CCR5-△32,CCR2-641 and SDF1-3' A variants was performed using PCR or PCR/RFLP assay,and further confirmed by direct DNA sequencing.Results Our finding shows that the△32 deletion mutation in the CCR5 gene does occur in this population and can be inherited in a Mendelian fashion in indigenous Han Chinese at a very low frequency of 0.00119(n= 1254).The frequencies of mutant CCR2-641 and SDF1-3'A alleles were 0.20023(n = 1251)and 0.2873(n = 893),in this population,which are higher than those found in American Caucasians.Furthermore the polymorphisms of CCR2-641 and SDF1-3' A alleles in the Han Chinese population were different from those in American Caucasians.Statistical analysis showed that the genotype distribution of CCR5-△32,CCR2-641 and SDF1-3' A alleles was in equilibrium according to the Hardy-Weinberg equation.Conclusion The CCR5-△32 mutation may not be a major resistant factor against HIV-1 infection in indigenous Han Chinese.The significance of higher frequencies of CCR2-641 and SDF1-3' A alleles (0.20023 and 0.2791)in the Han population remains to be clarified in HIV-1-positive carriers and AIDS patients.

Objective　To detect the genotypes and sequences of the exon 1 of human mannose-binding protein (MBP) allele in 5 Chinese nationalities. Methods　 The genotypes of MBP gene of 5 Chinese nationalities were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The exon 1 of the MBP gene of 22 Chinese Hans was analyzed by using ABI 310 genetic analyzer. Results　 The DNA sequences of exon 1 of Chinese MBP gene were acquired. The allele frequencies of the codon 54 of the MBP gene (MBP-54) of 5 Chinese nationalities were 0.181(Hans), 0.128(Uygurs), 0.181(Mongols), 0.179(Tibetans) and 0.181(Yis). The allele distribution for MBP-54 mutation of 5 Chinese nationalities was in good agreement with Hardy-Weinberg equilibrium. Compared with the Hans, Uygurs had a lower MBP-54 mutation rate. There were no differences in the allele frequencies between the chronic hepatitis B patients and health controls in Chinese Hans. The mutations of the codons 52 and 57 were not detected in this study. Conclusion　 A higher prevalence of MBP-54 mutation was found in 5 Chinese nationalities, MBP-54 mutation was not associated with the persistence of hepatitis B.