[Objective]To analyze the academic characteristics in Verse on Jade-Dragon Acupoints(Yulong Ge)from Great Compendium of Acupuncture and Moxibustion(Zhenjiu Dacheng).[Methods]Taking Zhenjiu Dacheng:Yulong Ge as the research object,the academic characteristics of Zhenjiu Dacheng:Yulong Ge are explored by comparing it longitudinally with Bianque Shenying Zhenjiu Yulongjing:Great Hundred and Twenty Points Yulong Ge and the independent book Yulong Ge.[Results]Comparison showed Zhenjiu Dacheng:Yulong Ge supplemented details diseases and syndromes,emphasized tonification and sedation,with more advocates of"tonifying first and then sedating,more tonification and less sedation";added two new cases of trans-acupoint needling based on Yulong Jing,corrected the error of"Touwei through Xuanzhong"in Yulongjing;paid attention to acupuncture and moxibustion contraindications;standardized the size of moxa cones,proposed"twenty-one moxa cones"for Gaohuang moxibustion,which was in line with clinical practice;composed Shengyu Ge,simplifying the content,condensing the length,diseases and acupoints,which has an advantage in dissemination.[Conclusion]Rooted in clinical practice,combining his own academic ideas and clinical experience,integrating disease and syndrome differentiation,trans-acupoint needling techniques and standardization of moxibustion dosage,YANG made revisions to the Yulong Ge,which not only inherited the essence of DOU's acupuncture method,but also innovated to meet clinical needs and reflected YANG's academic characteristics.

[Objective]To analyze the academic characteristics in Verse on Jade-Dragon Acupoints(Yulong Ge)from Great Compendium of Acupuncture and Moxibustion(Zhenjiu Dacheng).[Methods]Taking Zhenjiu Dacheng:Yulong Ge as the research object,the academic characteristics of Zhenjiu Dacheng:Yulong Ge are explored by comparing it longitudinally with Bianque Shenying Zhenjiu Yulongjing:Great Hundred and Twenty Points Yulong Ge and the independent book Yulong Ge.[Results]Comparison showed Zhenjiu Dacheng:Yulong Ge supplemented details diseases and syndromes,emphasized tonification and sedation,with more advocates of"tonifying first and then sedating,more tonification and less sedation";added two new cases of trans-acupoint needling based on Yulong Jing,corrected the error of"Touwei through Xuanzhong"in Yulongjing;paid attention to acupuncture and moxibustion contraindications;standardized the size of moxa cones,proposed"twenty-one moxa cones"for Gaohuang moxibustion,which was in line with clinical practice;composed Shengyu Ge,simplifying the content,condensing the length,diseases and acupoints,which has an advantage in dissemination.[Conclusion]Rooted in clinical practice,combining his own academic ideas and clinical experience,integrating disease and syndrome differentiation,trans-acupoint needling techniques and standardization of moxibustion dosage,YANG made revisions to the Yulong Ge,which not only inherited the essence of DOU's acupuncture method,but also innovated to meet clinical needs and reflected YANG's academic characteristics.

Objective To investigate the distribution and antimicrobial resistance profiles of the common pathogens isolated from urine from 2015 to 2021 in the CHINET Antimicrobial Resistance Surveillance Program.Methods The bacterial strains were isolated from urine and identified routinely in 51 hospitals across China in the CHINET Antimicrobial Resistance Surveillance Program from 2015 to 2021.Antimicrobial susceptibility was determined by Kirby-Bauer method,automatic microbiological analysis system and E-test according to the unified protocol.Results A total of 261 893 nonduplicate strains were isolated from urine specimen from 2015 to 2021,of which gram-positive bacteria accounted for 23.8％(62 219/261 893),and gram-negative bacteria 76.2％(199 674/261 893).The most common species were E.coli(46.7％),E.faecium(10.4％),K.pneumoniae(9.8％),E.faecalis(8.7％),P.mirabilis(3.5％),P.aeruginosa(3.4％),SS.agalactiae(2.6％),and E.cloacae(2.1％).The strains were more frequently isolated from inpatients versus outpatients and emergency patients,from females versus males,and from adults versus children.The prevalence of ESBLs-producing strains in E.coli,K.pneumoniae and P.mirabilis was 53.2％,52.8％and 37.0％,respectively.The prevalence of carbapenem-resistant strains in E.coli,K.pneumoniae,P.aeruginosa and A.baumannii was 1.7％,18.5％,16.4％,and 40.3％,respectively.Lower than 10％of the E.faecalis isolates were resistant to ampicillin,nitrofurantoin,linezolid,vancomycin,teicoplanin and fosfomycin.More than 90％of the E.faecium isolates were ressitant to ampicillin,levofloxacin and erythromycin.The percentage of strains resistant to vancomycin,linezolid or teicoplanin was＜2％.The E.coli,K.pneumoniae,P.aeruginosa and A.baumannii strains isolated from ICU inpatients showed significantly higher resistance rates than the corresponding strains isolated from outpatients and non-ICU inpatients.Conclusions E.coli,Enterococcus and K.pneumoniae are the most common pathogens in urinary tract infection.The bacterial species and antimicrobial resistance of urinary isolates vary with different populations.More attention should be paid to antimicrobial resistance surveillance and reduce the irrational use of antimicrobial agents.

Genomic studies of cancer cell alterations,such as mutations,copy number variations(CNVs),and translocations,greatly promote our understanding of the genesis and development of cancers.However,the 3D genome architecture of cancers remains less studied due to the complexity of cancer genomes and technical difficulties.To explore the 3D genome structure in clin-ical lung cancer,we performed Hi-C experiments using paired normal and tumor cells harvested from patients with lung cancer,combining with RNA sequenceing analysis.We demonstrated the feasibility of studying 3D genome of clinical lung cancer samples with a small number of cells(1×104),compared the genome architecture between clinical samples and cell lines of lung cancer,and identified conserved and changed spatial chromatin structures between normal and cancer sam-ples.We also showed that Hi-C data can be used to infer CNVs and point mutations in cancer.By integrating those different types of cancer alterations,we showed significant associations between CNVs,3D genome,and gene expression.We propose that 3D genome mediates the effects of cancer genomic alterations on gene expression through altering regulatory chromatin structures.Our study highlights the importance of analyzing 3D genomes of clinical cancer samples in addition to cancer cell lines and provides an integrative genomic analysis pipeline for future larger-scale studies in lung cancer and other cancers.

Objective To analyze the bone metabolism in hospitalized patients with Graves disease and the changes after 131I therapy.Methods The differences of bone metabolism were analyzed between 315 patients with Graves disease and 300 normal controls in a case-control study.The changes in bone turnover markers and BMD levels before and one year after 131I therapy were observed in 60 patients.Results Compared to normal control,bone turnover markers were markly higher and BMD levels were lower in patients with Graves disease.The level of thyroid hormones were positively related to bone turnover markers,while negatively related to total hip BMD (Z-score).But there was no linear relationship with lumbarand femoral neck BMD (Z-score).After one year of 131I therapy,bone turnover markers were markly lower than that before treatment,while BMD levels were partly higher than that before treatment.Conclusions In Graves disease patients,bone turnover markers were generally increased,while BMD levels decreased compared with normal people.After 131I therapy,along with the improvement of thyrotoxicosis,the high bone turnover rate can be suppressed,and BMD can partly recover.

Objective:To explore the clinical characteristics, mechanism and countermeasures of drug-induced second thrombocy-tosis ( ST) in order to ensure safe medication. Methods:Drug-induced ST published during 1996 and 2016 in domestic and overseas medical journals was collected and statistically analyzed in respects of age, gender, kind and distribution of drug-induced ST, treatment and outcome of ST. Results:Totally 198 cases were collected, which involved 30 different drugs of eight types mainly including antitu-mor drugs, blood system drugs and antibiotics. Among the patients, 112 ones (56. 6％) were male and the other 86 ones (43. 4％) were female. Totally 14 patients had embolization with the overall incidence of 7. 0％. Conclusion:Without timely and favorable treat-ment, drug-induced ST may lead to thrombosis. To ensure the safety of medication, platelet level should be monitored regularly during medication.

A 76-year-old man with bronchopneumonia received an IV infusion of ceftizoxime sodium 2 g twice daily.Before treatment, laboratory tests showed the following results: white blood cell count (WBC) 10.8×109/L, neutrophil 0.94, red blood cell count (RBC) 3.8×1012/L, hemoglobin (Hb) 121 g/L, total bilirubin (TBil) 12.6 μmol/L, indirect bilirubin (IBil) 8.7 μmol/L, serum creatinine (Scr) 151 μmol/L, blood urea nitrogen (BUN) 10.7 mmol/L, endogenous creatinine clearance rate 35 ml/min.On day 11, about 10 minutes after beginning infusion of the 22nd dosage of ceftizoxime, he developed low back pain with bladder distending pain and yellowish skin and sclera.Ceftizoxime sodium was withdrawn immediately.Urethral catheter drained soy sauce-colored urine 200 ml.Laboratory tests revealed the following results: WBC 15.7×109/L, neutrophil 0.68, RBC 1.1×1012/L, Hb 73 g/L, reticulocytes 0.023, TBil 83.8 μmol/L, IBil 61.8 μmol/L, Scr 127 μmol/L, BUN 11.9 mmol/L, D-dimer 9.8 mg/L, direct Coomb′s test positive, urine bilirubin (++), occult blood (++), 2-3 red blood cells per high power field.He received treatments such as hydration and urinary alkalinization, infusion of methylprednisolone, transfusion of washed red blood cells and etc.However, he had sustained low urine output, progressive jaundice, and finally died 8 hours later.Acute hemolytic anemia was considered to be induced by overdose of ceftizoxime sodium.

A 76-year-old man with bronchopneumonia received an IV infusion of ceftizoxime sodium 2 g twice daily.Before treatment, laboratory tests showed the following results: white blood cell count (WBC) 10.8×109/L, neutrophil 0.94, red blood cell count (RBC) 3.8×1012/L, hemoglobin (Hb) 121 g/L, total bilirubin (TBil) 12.6 μmol/L, indirect bilirubin (IBil) 8.7 μmol/L, serum creatinine (Scr) 151 μmol/L, blood urea nitrogen (BUN) 10.7 mmol/L, endogenous creatinine clearance rate 35 ml/min.On day 11, about 10 minutes after beginning infusion of the 22nd dosage of ceftizoxime, he developed low back pain with bladder distending pain and yellowish skin and sclera.Ceftizoxime sodium was withdrawn immediately.Urethral catheter drained soy sauce-colored urine 200 ml.Laboratory tests revealed the following results: WBC 15.7×109/L, neutrophil 0.68, RBC 1.1×1012/L, Hb 73 g/L, reticulocytes 0.023, TBil 83.8 μmol/L, IBil 61.8 μmol/L, Scr 127 μmol/L, BUN 11.9 mmol/L, D-dimer 9.8 mg/L, direct Coomb′s test positive, urine bilirubin (++), occult blood (++), 2-3 red blood cells per high power field.He received treatments such as hydration and urinary alkalinization, infusion of methylprednisolone, transfusion of washed red blood cells and etc.However, he had sustained low urine output, progressive jaundice, and finally died 8 hours later.Acute hemolytic anemia was considered to be induced by overdose of ceftizoxime sodium.

Objective:To investigate the pharmaceutical care methods for the patients with drug-induced liver injury. Methods:The participation process of clinical pharmacists in 3 cases of typical drug-induced liver injury was with detailed introduction and analy-sis in the paper. Results:The case 1 indicated that new drugs probably with drug-induced liver injury should be warned in the process of clinical medication. The case 2 suggested that TDM, as a useful assessment weapon, could be fully used to find the source medi-cines when drug-induced liver injury occurred. The case 3 showed the specific cases, especially the patients with abnormal liver func-tion, should be focused on, the medicines with high liver toxicity should be avoided and the medicines with mild liver toxicity could be chosen. Conclusion:Clinical pharmacists should participate in the clinical practice of drug-induced liver injury with multi-channel and multi-link, and pay attention to the drugs with high risk of liver injury. Meanwhile, clinical pharmacists should perform TDM monito-ring to provide positive evidence for the diagnosis of drug-induced liver injury, and focus on the liver and kidney functions to provide better pharmaceutical care for the patients.

[Summary] Dyslipidemia after organ transplantation is one of the important risk factors of postoperative cardiovascular disease and graft dysfunction. There are many factors that result in postoperative dyslipidemia. However, the factors influencing serum lipid levels are changing with the development of organ transplantation. In this article the effects of different anti-rejection drugs such as cyclosporine, azathioprine, mycophenolate mofetil, tacrolimus, rapamycin ( sirolimus ) , corticosteroids, and monoclonal antibody on dyslipidemia after organ transplantation were summarized in different eras.