OBJECTIVE:In recent years,the number of clinical research reports on acupuncture and manipulation for the treatment of greater occipital neuralgia has gradually increased,but the quality is uneven.There is currently no literature evaluating the quality of published reports,which is not conducive to the promotion of clinical use of these therapies.Therefore,this article assessed the reporting quality of randomized controlled trials on acupuncture and manipulation for greater occipital neuralgia.METHODS:Cochrane Library,PubMed,Web of Science,Embase,China National Knowledge Infrastructure(CNKI),VIP,WanFang Data,and Chinese BioMedical Literature Database(CBM)from inception to May 20,2024 were searched.The reporting quality of included randomized controlled trials was independently evaluated by two investigators using the CONSORT statement,STRICTA checklist,and Cochrane bias of risk assessment tool.A third investigator resolved any disagreement.RESULTS:A total of 62 articles were included.Based on the CONSORT statement,59.46％(22/37)of all entries had a reporting rate of less than 50％,mainly including"Identification as a randomized trial in the title(1/62,1.61％),""How sample size was determined(7/62,11.29％),""Implementation(1/62,1.61％),""Blinding(1/62,1.61％),"and"Reports of Funding(4/62,6.45％)."According to the STRICTA checklist,29.41％(5/17)of all entries had a reporting rate of less than 50％,mainly including"Details of other interventions(7/58,12.07％),""Setting and context of treatment(0/58,0％),"and"Description of participating acupuncturists(0/58,0％)."CONCLUSION:The reporting quality of randomized controlled trials on acupuncture and manipulation therapy for greater occipital neuralgia remains low.Future researchers need to make greater efforts to strictly adhere to the CONSORT statement and STRICTA checklist during trial design,implementation,and reporting.This will facilitate the standardization of research in this field and enhance the reliability and reproducibility of the research results.

Objective To compare the short-term clinical outcomes between Natural Orifice Specimen Extraction Surgery(NOSES)and conventional laparoscopic surgery(CLS)for rectal cancer.Methods Clini-cal data of 120 patients with rectal cancer admitted to this hospital from May 2021 to May 2023 were retro-spectively analyzed.They were divided into a NOSES group and a CLS group(60 cases each)according to sur-gical approach.Short-term efficacy of both surgical approaches was analyzed.Results The NOSES group had longer operative time,shorter postoperative hospital stay,and lower incision infection rate than the CLS group(P<0.05).Serum CRP levels in the NOSES group were lower than those in the CLS group on postoperative days 1,3,and 5(P<0.05).VAS scores in the NOSES group were lower than those in the CLS group at 6 hours and days 1-7 postoperatively(P<0.05).The proportion of patients requiring additional dezocine anal-gesia was lower in the NOSES group at 6 hours and days 1-4 postoperatively(P<0.05).CEA levels gradu-ally decreased over time in both groups(P<0.05),but showed no significant intergroup difference(P>0.05).Conclusion Compared with CLS,NOSES has advantages of less postoperative pain,faster recovery,and shorter hospital stay for rectal cancer patients.

Objective To analyze the modeling and evaluation method for vascular restenosis animal models in the last 10 years,to provide a reference for improving animal models of vascular restenosis.Methods Literature related to vascular restenosis was retrieved from mainstream Chinese and English databases from 2013 to 2023.Data on experimental animal strains,modeling method,modeling cycles,and detection method were extracted from the included literature,and a database was established using Excel for summary analysis.Results Among the 122 identified articles,the main experimental animals were rats,rabbits,and pigs,and most animals were male.The most common modeling method was balloon injury,and the modeling cycle was mainly within 4～8 weeks.The main detection indexes were histopathology,accounting for 37.18％,including routine hematoxylin-eosin,Masson,and Elastica van Gieson(EVG)staining.Conclusions The translatability of porcine vascular restenosis models is currently more in line with expectations,but their cost is high and they are unpopular,and rats and rabbits thus remain the main animal models.Balloon injury is the main mode of modeling.Different animal models and modeling method for vascular restenosis have advantages and disadvantages,and the model should be selected according to the experimental purpose.Animal models of vascular restenosis still have some limitations,however,and better animal models are required in the future.

OBJECTIVE:In recent years,the number of clinical research reports on acupuncture and manipulation for the treatment of greater occipital neuralgia has gradually increased,but the quality is uneven.There is currently no literature evaluating the quality of published reports,which is not conducive to the promotion of clinical use of these therapies.Therefore,this article assessed the reporting quality of randomized controlled trials on acupuncture and manipulation for greater occipital neuralgia.METHODS:Cochrane Library,PubMed,Web of Science,Embase,China National Knowledge Infrastructure(CNKI),VIP,WanFang Data,and Chinese BioMedical Literature Database(CBM)from inception to May 20,2024 were searched.The reporting quality of included randomized controlled trials was independently evaluated by two investigators using the CONSORT statement,STRICTA checklist,and Cochrane bias of risk assessment tool.A third investigator resolved any disagreement.RESULTS:A total of 62 articles were included.Based on the CONSORT statement,59.46％(22/37)of all entries had a reporting rate of less than 50％,mainly including"Identification as a randomized trial in the title(1/62,1.61％),""How sample size was determined(7/62,11.29％),""Implementation(1/62,1.61％),""Blinding(1/62,1.61％),"and"Reports of Funding(4/62,6.45％)."According to the STRICTA checklist,29.41％(5/17)of all entries had a reporting rate of less than 50％,mainly including"Details of other interventions(7/58,12.07％),""Setting and context of treatment(0/58,0％),"and"Description of participating acupuncturists(0/58,0％)."CONCLUSION:The reporting quality of randomized controlled trials on acupuncture and manipulation therapy for greater occipital neuralgia remains low.Future researchers need to make greater efforts to strictly adhere to the CONSORT statement and STRICTA checklist during trial design,implementation,and reporting.This will facilitate the standardization of research in this field and enhance the reliability and reproducibility of the research results.

Objective To analyze the modeling and evaluation method for vascular restenosis animal models in the last 10 years,to provide a reference for improving animal models of vascular restenosis.Methods Literature related to vascular restenosis was retrieved from mainstream Chinese and English databases from 2013 to 2023.Data on experimental animal strains,modeling method,modeling cycles,and detection method were extracted from the included literature,and a database was established using Excel for summary analysis.Results Among the 122 identified articles,the main experimental animals were rats,rabbits,and pigs,and most animals were male.The most common modeling method was balloon injury,and the modeling cycle was mainly within 4～8 weeks.The main detection indexes were histopathology,accounting for 37.18％,including routine hematoxylin-eosin,Masson,and Elastica van Gieson(EVG)staining.Conclusions The translatability of porcine vascular restenosis models is currently more in line with expectations,but their cost is high and they are unpopular,and rats and rabbits thus remain the main animal models.Balloon injury is the main mode of modeling.Different animal models and modeling method for vascular restenosis have advantages and disadvantages,and the model should be selected according to the experimental purpose.Animal models of vascular restenosis still have some limitations,however,and better animal models are required in the future.

Objective: As the prevention and control of COVID-19continues to advance, the active nucleic acid test screening in the close contacts of the patients has been carrying out in many parts of China. However, the false-positive rate of positive results in the screening has not been reported up to now. But to clearify the false-positive rate during screening is important in COVID-19 control and prevention. Methods: Point values and reasonable ranges of the indicators which impact the false-positive rate of positive results were estimated based on the information available to us at present. The false-positive rate of positive results in the active screening was deduced, and univariate and multivariate-probabilistic sensitivity analyses were performed to understand the robustness of the findings. Results: When the infection rate of the close contacts and the sensitivity and specificity of reported results were taken as the point estimates, the positive predictive value of the active screening was only 19.67%, in contrast, the false-positive rate of positive results was 80.33%. The multivariate-probabilistic sensitivity analysis results supported the base-case findings, with a 75% probability for the false-positive rate of positive results over 47%. Conclusions In the close contacts of COVID-19 patients, nearly half or even more of the 'asymptomatic infected individuals’ reported in the active nucleic acid test screening might be false positives.

Objective:By analyzed the transmission patterns of 4 out of the 51 COVID-19 cluster cases in Shaanxi province to provide evidences for the COVID-19 control and prevention.Methods:The epidemiological data of RT-PCR test-confirmed COVID-19 cases were collected. Transmission chain was drawn and the transmission process was analyzed.Results:Cluster case 1 contained 13 cases and was caused by a family of 5 who traveled by car to Wuhan and returned to Shaanxi. Cluster case 2 had 5cases and caused by initial patient who participated family get-together right after back from Wuhan while under incubation period. Cluster case 3 contained 10 cases and could be defined as nosocomial infection. Cluster case 4 contained 4 cases and occurred in work place.Conclusion:Higher contact frequency and smaller places were more likely to cause a small-scale COVID-19 cluster outbreak, with potential longer incubation period. COVID-19 control strategies should turn the attention to infection prevention and control in crowded places, management of enterprise resumption and prevention of nosocomial infection.

Objective　To investigate neural functional recovery and intracranial vascular disease of acute cerebral infarct (ACI) patients complicated with polyvascular disease (PolyVD).Methods　Retrospective analysis was conducted on 83 ACI patients with monovascular disease and 68 ACI patients with polyvascular disease seeking hospitalization in the Neurology Department of the Third Affiliated Hospital of Jinzhou Medical University from January 2019 to September 2019.The changes of intracranial artery disease and neural functional recovery of the monovascular disease group and polyvascular disease group were analyzed,and multiple factors that affect neural function recovery were analyzed.Results　(1)Compared with the monovascular disease group,the PolyVD group was found with a higher percentage of poor neural functional recovery,lower recovery rate of neural functions,and a larger number of vessels associated with the intracranial disease.The differences were statistically significan（P<0.05）.(2)The number of vessels associated with the intracranial disease and the number of vascular beds were positively correlated（P<0.05）,while the neural functional recovery rate and the number of vascular beds were negatively correlated（P<0.05）.(3)Age,medical history of diabetes and stroke,and number of vascular beds were all independent risk factors of poor neural functional recovery.Conclusions　Polyvascular disease is an independent risk factors of poor neural functional recovery among ACI patients.The higher the number of vascular beds of the disease complicated with carotid atherosclerosis is,the higher the possibility of poor neural functional recovery will be,and the worse the intracranial artery disease will be.

Objective To study the relation between alcohol-induced osteonecrosis of femoral head (AIONFH) related with high morbidity TCM constitution type with CYP2C8 gene polymorphisms.Methods Totally 152 Han nationality NONFH cases from Feburary 2014 to September 2015 from outpatient and the inpatient departments in Gansu Province Hospital of TCM were collected. 50 AIONFH cases were set as medical case group; meanwhile, 45 healthy volunteers were enrolled as control group. Database for medical materials of all patients and volunteers was established. TCM distribution for AIONFH patients was determined. Solution DNA extraction kit was used to extract DNA, and detect the concentration and purity of DNA. The target gene was amplified by PCR and the target gene was amplified by gel electrophoresis. The length of the fragment was confirmed to conduct target gene sequencing. With the results of sequencing and gel electrophoresis, the relation of AIONFH with CYP2C8 gene polymorphism in AIONFH patients with phlegm-dampness syndrome and the control group.ResultsThe CYP2C8 gene loci rs17110453 gene polymorphism was not statistically significant between the two groups (χ2=0.253,P>0.05). There was no significant difference in allele between the two groups (χ2=0.077,P>0.05). The risk of disease in CC genotype was 1.37 times higher than the AA genotype (95%CI: 0.339-5.540), without statistical significance (P>0.05). There was no significant difference in genotype and allele distribution between AIONFH patients with phlegm-dampness and non-phlegm-dampness and the control group (P>0.05).Conclusion CYP2C8 gene loci rs17110453 gene polymorphism A/C mutation has no obvious relation with AIONFH risk. There is no clear relationship between CYP2C8 gene loci rs17110453 gene polymorphism with AIONFH.

Objective To study the correlation between nontraumatic osteonecrosis of femoral head (NONFH) and ApoA1 polymorphism of blood stasis type.Methods Totally 93 cases of NONFH were selected as the case group, and 83 healthy volunteers were randomly selected as the control group. With TCM constitution questionnaire survey, the case group was screened out 32 cases of blood stasis NONFH type and 61 cases of non blood stasis NONFH type. In the case group and control group, the subjects took blood samples 2 mL, extracted DNA for PCR amplification, PCR products for DNA sequencing. G994T PAF-AH and rs9658282 gene NOS1 site polymorphism were detected for tatistical analysis.Results -75G/A gene AA ApoA1 genotype (OR: 2.578; 95%CI: 1.174-5.663;P=0.018) and A allele (OR: 1.726; 95%CI: 1.121-2.658;P=0.013) may be one of the risk factors of NONFH.Conclusion -75G/A gene ApoA1 may be related to the pathogenesis of NONFH. There was no correlation between the ApoA1 gene polymorphism of -75G/A gene and the pathogenesis of blood stasis NONFH. There was no correlation between the ApoA1 gene polymorphism of +83C/T gene and the pathogenesis of blood stasis NONFH.