Objective To systematically analyze the research status,hotspots,and trends in the field of health-care-associated infection(HAI)in 2020-2025 globally,with a focus on exploring the development strategy of sci-entific and technological talents,and provide data support for talent cultivation,disciplinary layout,and evidence-based decision-making in this field.Methods Web of Science Core Collection was adopted as data source,and HAI-related literatures from January 2020 to January 2025 were retrieved.Bibliometric methods were employed with tools such as VOSviewer and Bibliometrix(R4.4.0)to conduct a cluster analysis on annual publication volume,countries/regions,authors,highly cited literatures,and keywords.The distribution characteristics,collaboration modes,and adaptability to research hotspots of scientific and technological talents in this field were also sorted out.Results A total of 9 566 literatures were included in the analysis.The annual publication volume peaked in 2021 and subsequently entered a high-level platform period with over 1 800 articles per year.China and the United States formed a dual-core research pattern,with Chinese scholars dominating the list of productive authors.Highly cited literatures were predominantly published in 2020-2021,mainly focusing on the clinical characteristics and HAI risks during the early stages of the COVID-19 pandemic.Research hotspots present a dual theme of"clinical epide-miology as well as management of prevention and control"and"antimicrobial resistance mechanisms of pathogens".However,the existing talent system shows deficiency in interdisciplinary adaptability and the depth of international collaboration.Conclusion Over the past five years,global research on HAI has entered a normalized stage,and Chinese scholars play vital role in the dual-core pattern of China and the United States.From the perspective of ta-lents,it is necessary to formulate a development strategy for scientific and technological talents around research hotspots,including improving transnational collaboration mechanisms,innovating interdisciplinary training models,and establishing a dynamic adaptability evaluation system,so as to provide action guidance and policy references for talent development in this field.

Objective To systematically analyze the research status,hotspots,and trends in the field of health-care-associated infection(HAI)in 2020-2025 globally,with a focus on exploring the development strategy of sci-entific and technological talents,and provide data support for talent cultivation,disciplinary layout,and evidence-based decision-making in this field.Methods Web of Science Core Collection was adopted as data source,and HAI-related literatures from January 2020 to January 2025 were retrieved.Bibliometric methods were employed with tools such as VOSviewer and Bibliometrix(R4.4.0)to conduct a cluster analysis on annual publication volume,countries/regions,authors,highly cited literatures,and keywords.The distribution characteristics,collaboration modes,and adaptability to research hotspots of scientific and technological talents in this field were also sorted out.Results A total of 9 566 literatures were included in the analysis.The annual publication volume peaked in 2021 and subsequently entered a high-level platform period with over 1 800 articles per year.China and the United States formed a dual-core research pattern,with Chinese scholars dominating the list of productive authors.Highly cited literatures were predominantly published in 2020-2021,mainly focusing on the clinical characteristics and HAI risks during the early stages of the COVID-19 pandemic.Research hotspots present a dual theme of"clinical epide-miology as well as management of prevention and control"and"antimicrobial resistance mechanisms of pathogens".However,the existing talent system shows deficiency in interdisciplinary adaptability and the depth of international collaboration.Conclusion Over the past five years,global research on HAI has entered a normalized stage,and Chinese scholars play vital role in the dual-core pattern of China and the United States.From the perspective of ta-lents,it is necessary to formulate a development strategy for scientific and technological talents around research hotspots,including improving transnational collaboration mechanisms,innovating interdisciplinary training models,and establishing a dynamic adaptability evaluation system,so as to provide action guidance and policy references for talent development in this field.

Objective To explore the possible mechanism of apoptosis induced by SARS-CoV-2 targeting cyclophilin A(CypA)/CD147 receptor pathway.Methods The pEncmv-SARS-CoV-2 S-3xflag recombinant plasmid was constructed and transfected into H9C2 cells.Apoptosis was analyzed by TUNEL assay,flow cytometry and DNA ladder.The interaction between CD147 and SARS-CoV-2 S protein was verified by CoIP.The expressions of apoptosis-related proteins were detected by Western blotting.Results Compared with the control group,TUNEL assay and flow cytometry showed that apoptosis was significantly increased in transfection group(P＜0.05),and DNA ladder analysis showed that DNA degradation was obvious.The expression levels of Caspase12 and DR3 were significantly increased(P＜0.001),the expression level of FAS was slightly increased(P＜0.01),while the expression levels of TRF1 and DR4 were decreased(P＜0.01).Conclusion SARS-CoV-2 S protein targets CypA/CD147 receptor to invade host cells,activate endoplasmic network pathway(Caspase12)and death receptor pathway(DR3,FAS),and induce apoptosis of cardiomyocytes through endogenous and exogenous apoptotic pathways.

Objective To describe the general demographic characteristics of the breastfeeding self -efficacy, the differences in social support and study their correlation .Methods Convenience selection method was adopted from August to December , 2012 , and 154 cases joined the study .Research tools included a demographic characteristics questionnaire , the Breastfeeding Self-Efficacy Scale , and the Social Support Scale . SPSS 17.0 was used for data analysis .Results The new mothers in different general demographic characteristics had no significant differences in self-efficacy (P>0.05).The scores of social support of new mothers with middle school education background , junior college background and university background were (39.34 ±4.17), (42.16 ±5.55) and (46.85 ±4.96), respectively.The difference was significant (F=13.940, P<0.01).The scores of social support of new mothers with different working situations was significant different ( t =2.253, P <0.05).The mean score of breastfeeding self-efficacy and social support of new mothers were (110.71 ±19.53) and (42.73 ±5.73), respectively, with a positive correlation (r=0.235, P<0.01).The total score of social support and the score of skills and mental activity , and the scores of subjective feeling of social support and self-efficacy and the score of mental activity had a positive correlation (r=0.231, 0.243, 0.244, 0.230, respectively; P<0.01).Conclusions The early maternal breastfeeding self-efficacy scores are high and the social support is in good condition .Both the Han and the Korean new mothers have gained an excellent self-efficacy.