BACKGROUND: The sirtuin family is well recognized for its crucial involvement in various cellular processes. Nevertheless, studies on its role in the human endometrium are limited. This study aimed to explore the expression and localization of the sirtuin family in the human endometrium, focusing on sirtuin 3 (SIRT3) and its potential role in the oxidative imbalance of the endometrium in polycystic ovary syndrome (PCOS). METHODS: Endometrial specimens were collected from both patients with PCOS and controls undergoing hysteroscopy at the Center for Reproductive Medicine, Peking University Third Hospital, from July to August 2015 and used for cell culture. The protective effects of SIRT3 were investigated, and the mechanism of SIRT3 in improving endometrial receptivity of patients with PCOS was determined using various techniques, including cellular bioenergetic analysis, small interfering ribonucleic acid (siRNA) silencing, real-time quantitative polymerase chain reaction, Western blot, immunofluorescence, immunohistochemistry, and flow cytometry analysis. RESULTS: The sirtuin family was widely expressed in the human endometrium, with SIRT3 showing a significant increase in expression in patients with PCOS compared with controls ( P <0.05), as confirmed by protein and gene assays. Concurrently, endometrial antioxidant levels were elevated, while mitochondrial respiratory capacity was reduced, in patients with PCOS ( P <0.05). An endometrial oxidative stress (OS) model revealed that the downregulation of SIRT3 impaired the growth and proliferation status of endometrial cells and reduced their receptivity to day 4 mouse embryos. The results suggested that SIRT3 might be crucial in maintaining normal cellular state by regulating antioxidants, cell proliferation, and apoptosis, thereby contributing to enhanced endometrial receptivity. CONCLUSIONS Our findings proposed a significant role of SIRT3 in improving endometrial receptivity in patients with PCOS by alleviating OS and regulating the balance between cell proliferation and apoptosis. Therefore, SIRT3 could be a promising target for predicting and improving endometrial receptivity in this patient population.
Humans ; Female ; Polycystic Ovary Syndrome/metabolism* ; Endometrium/metabolism* ; Sirtuin 3/genetics* ; Oxidative Stress/genetics* ; Adult ; Animals ; Mice ; Apoptosis/physiology* ; Immunohistochemistry ; Cell Proliferation/physiology*

Objective:To develop the Exercise Knowledge and Behavior Questionnaire for Osteoporosis Patients suitable for China, and to test its reliability and validity.Methods:Guided by the theory of knowledge, attitude and practice, a questionnaire framework was constructed, and the first draft of the Exercise Knowledge and Behavior Questionnaire for Osteoporosis Patients was formed through literature review, group discussion, semi-structured interview, Delphi expert consultation and pre-investigation. From June to August 2020, convenience sampling was used to select 120 osteoporosis patients admitted to the First Affiliated Hospital of Soochow University as the research object to conduct a questionnaire survey to test the reliability and validity of the questionnaire. A total of 120 questionnaires were distributed, 110 valid questionnaires were recovered, and the valid recovery rate was 91.67% (110/120) .Results:Among 2 rounds of Delphi expert consultation, the expert authority coefficients were 0.880 and 0.900, respectively, and the Kendall harmony coefficients were 0.308 and 0.332, respectively, with statistically significant differences ( P<0.01) . The Exercise Knowledge and Behavior Questionnaire for Osteoporosis Patients included two sub-questionnaire of knowledge and behavior, with a total of 19 items, including 10 items in knowledge and 9 items in behavior. Exploratory factor analysis showed that 2 common factors were extracted from the exercise knowledge sub-questionnaire, and the cumulative variance contribution rate was 60.712%. A total of one common factor was extracted for the exercise behavior sub-questionnaire, and the cumulative variance contribution rate was 72.075%. The content validity index of the total questionnaire and each sub-questionnaire were 0.925, 0.921, and 0.929, respectively, and the item-level content validity index of was 0.857 to 1.000. The Cronbach's α coefficients of the total questionnaire and each sub-questionnaire t were 0.935, 0.830, and 0.953, respectively, and the split-half reliability coefficients of the total questionnaire and each sub-questionnaire were 0.979, 0.898, and 0.974, respectively, and the test-retest reliability coefficients of the total questionnaire and each sub-questionnaire were 0.878, 0.813, 0.860, respectively. Conclusions:The Exercise Knowledge and Behavior Questionnaire for Osteoporosis Patients has good reliability and validity, and can be used to evaluate exercise knowledge and behavior in patients with osteoporosis.

Objective To explore the main factors of the medical communication between physicians and nurses in gynecological emergency treatments. To evaluate the function of SBAR communication mode in enhancing communication skills and to explore the countermeasures of promotion. Methods Totally 18 gynecological physicians and nurses were selected as the subjects in this study,and semi-structured interviews were conducted. After the establishment of coding and archiving system, the collected information was extracted and analyzed. Results The factors that affect gynecologic emergency medical staff effective communication included assessment of the patients′ condition, the limitation of communication conditions,the different concerns between doctors and nurses,the differences of medical communication training degree,seniority,experience and knowledge reserve,the medical team consciousness. Conclusion The effective communication of gynecologic emergency care can be promoted by improving professional levels of medical care personnel,strengthening the cultivation of the health care team,using communication skills,to ensure the accurate transfer of key information.

Objective To explore the influence of two continuing care methods on the rehabilitation in female genital malignant tumor patients.Methods A total of 30 patients from June through December 2016 were included in control group which given continuing nursing care,another 30 patients from January through June 2017 were included in the experimental group which given continuing nursing care by WeChat.The urinary tract infection and quality of life of the two groups after chemotherapy were observed.Results The physiological status,social/family status,emotional status,quality of life score and treatment compliance of experimental group were higher than that in the control group,the incidence rate of urinary tract infection outside hospital of experimental group was lower than the control group (P ＜ 0.05).Conclusion The continuing nursing mode of WeChat can better improve the life quality of patients with malignant tumors,improve patients'compliance,reduce the incidence of urinary tract infection,and has the advantages of simplicity,economy,and lower visit lose.

Objective To explore the influence of two continuing care methods on the rehabilitation in female genital malignant tumor patients.Methods A total of 30 patients from June through December 2016 were included in control group which given continuing nursing care,another 30 patients from January through June 2017 were included in the experimental group which given continuing nursing care by WeChat.The urinary tract infection and quality of life of the two groups after chemotherapy were observed.Results The physiological status,social/family status,emotional status,quality of life score and treatment compliance of experimental group were higher than that in the control group,the incidence rate of urinary tract infection outside hospital of experimental group was lower than the control group (P ＜ 0.05).Conclusion The continuing nursing mode of WeChat can better improve the life quality of patients with malignant tumors,improve patients'compliance,reduce the incidence of urinary tract infection,and has the advantages of simplicity,economy,and lower visit lose.

Humanization is becoming increasingly significant inpatient-nurse collaboration. In China,the bed-nurse ratio is unbalanced,the medical dispute is serious and the patient′s satisfaction is not high.Narrative medicine which exertnalizing one′s experiences and emotion,are beneficial to patients′physical and mental rehabilitation. In narrative nursing, nurses entered into patients′ feeling and feel empathy,then provided higher quality nursing care.Narrative nursing effectively makes up for the lack of humanistic care. This article shows the development and shorts of humanistic care. Then discussed the practical application and limitations of narrative nursing as well as it′s significance of strengthening humanistic care.

Objective To explore the clinical value of the combination of procalcitonin (PCT) and hypersensitive C-reactive protein (hs-CRP) detection for the assessment of bacterial pneumonia.Methods Fifty bacterial pneumonia patients were randomly selected and included into the observation group,another fifty healthy subjects were selected as the control group in the same period.The levels of hs-CRP and PCT in the two groups were detected,and the detection results were comparatively analyzed.Results The PCT level [(3.13 ± 0.43) μg/L] and hs-CRP level[(55.37 ± 9.64)mg/L] in the observation group before treatment were significantly higher than those in the control group [PCT level (0.04 ± 0.01)μg/L,hs-CRP level (6.46 ± 0.89)mg/L],and the differences were statistically significant (t =50.7993,36.3980,all P ＜0.05).There were no significant differences in the PCT and hs -CRP levels between the two groups after treatment (t =0.5000,0.9444,all P ＞ 0.05).The PCT level (3.11 ±0.85) μg/L and hs-CRP level (20.31 ± 3.96) mg/L in the patients with CURB-65 score of 4-5 were significantly higher than those in patients with CURB-65 score of 0-1 and 2-3 [PCT level (1.19 ± 0.42) μg/L and hs-CRP level (1.86 ± 0.53) mg/L in patients with CURB-65 score of 0-1,PCT level (1.82 ± 0.54) μg/L and hs-CRP level (9.85 ± 1.21)mg/L in patients with CURB-65 score of 2-3],and the differences were statistically significant (t =8.3565,5.0788,20.8283,10.9947,all P ＜ 0.05).Conclusion The levels of serum PCT and hs-CRP are significantly increased in the patients with bacterial pneumonia,but the levels are decreased significantly after treatment.The combination of hs-CRP and PCT detection has significant value for the assessment of bacterial pneumonia,which is helpful to evaluate the condition of the disease,and has great clinical significance.

Objective To investigate risk factors for healthcare-associated infection(HAI)in patients with craniocerebral operation,and provide reference for the prevention and control of HAI.Methods A total of 4 246 cases of craniocerebral surgery in a neurosurgery department from January 2010 to December 2012 were enrolled in the study,the risk fac-tors for HAI were analyzed.Results Of 4 246 cases of craniocerebral surgery,393 patients developed 446 times of post-operative HAI,HAI incidence rate was 9.26%,case infection rate was 10.50%,which were higher than inci-dence (2.02%)and case infection rate (3.02%)of HAI of all hospital during the same period,the difference was statistically significant (χ2 =811 .06,629.30,respectively,P <0.001).The major infection site was central nervous system (56.50%),followed by respiratory system (27.36%).Unconditional univariate logistic regression analysis showed that sex,age,community-acquired infection,primary disease,operative time,length of stay in intensive care unit (ICU)before infection,the number of surgery,invasive procedures and nasogastric tube,the difference was statistically significant (all P <0.05 ).Multivariate logistic regression analysis showed that sex,elderly pa-tients,congenital brain diseases,stay in ICU>7 d,antimicrobial use >7 d,central venous and urinary tract cathe-terization,invasive ventilator were risk factors for HAI in patients with craniocerebral operation.Conclusion The incidence of HAI in patients with craniocerebral operation is high,effective preventive and control measures accord-ing to risk factors should be strengthened to reduce the incidence of HAI.