Objective To explore the preoperative diagnostic value of a radiomics nomogram based on intratumoral and peritumoral apparent diffusion coefficient(ADC)maps for prostate cancer.Methods A retrospective collection was conducted on MRI images of 503 patients with prostate lesions confirmed by pathology.The region of interest(ROI)was delineated on the ADC maps and extended 1-5 mm outward to form the peritumoral region.Radiomics features were extracted from both intratumoral and peritumoral regions,and radiomics models were established.A combined model integrating clinical model was constructed and a nomogram was drawn.The performance of each model and nomogram were evaluated.Results The combined model achieved the highest area under the curve(AUC)in the test set(AUC=0.823)at a peritumoral distance of 3 mm.The nomogram based on the combined model showed good predictive performance and clinical utility on both decision curve analysis(DCA)and calibration curve.Conclusion The radiomics nomogram based on intratumoral and peritumoral ADC maps has the greatest diagnostic value in distinguishing benign and malignant prostate cancer at a peritumoral distance of 3 mm before surgery.

Objective To explore the preoperative diagnostic value of a radiomics nomogram based on intratumoral and peritumoral apparent diffusion coefficient(ADC)maps for prostate cancer.Methods A retrospective collection was conducted on MRI images of 503 patients with prostate lesions confirmed by pathology.The region of interest(ROI)was delineated on the ADC maps and extended 1-5 mm outward to form the peritumoral region.Radiomics features were extracted from both intratumoral and peritumoral regions,and radiomics models were established.A combined model integrating clinical model was constructed and a nomogram was drawn.The performance of each model and nomogram were evaluated.Results The combined model achieved the highest area under the curve(AUC)in the test set(AUC=0.823)at a peritumoral distance of 3 mm.The nomogram based on the combined model showed good predictive performance and clinical utility on both decision curve analysis(DCA)and calibration curve.Conclusion The radiomics nomogram based on intratumoral and peritumoral ADC maps has the greatest diagnostic value in distinguishing benign and malignant prostate cancer at a peritumoral distance of 3 mm before surgery.

Objective:To improve the understanding of craniocerebral diseases and aeromedical assessment from the perspective of imaging by analyzing the results of craniocerebral MRI examination of military flying personnel.Methods:The results of craniocerebral MRI examination were retrospectively analyzed among the military flying personnel who underwent annual physical examination in Air Force Healthcare Center for Special Services Hangzhou from September of 2020 to December of 2021. They were divided into 20-29 years group, 30-39 years group, 40-49 years group, 50-59 years group according to the age, and they were divided into fighter pilots, bomb-transporter pilots, helicopter pilots, air combat/technical personnel according to the aircraft type and post. The detection of craniocerebral MRI examination results of the flying personnel in different age groups and aircraft type and post groups were compared. The influence of abnormal results on the conclusion of aeromedical assessment was analyzed. The α level of statistical analysis was set at 0.05, and the difference was statistically significant with P< α/statistical count for pairwise comparison among groups. Results:Among the craniocerebral MRI examination results of 975 military flying personnel, 473 cases were with no obvious abnormality, 194 were anatomical variation of Willis circle, 27 were congenital brain malformation, 54 were cerebrovascular disease, 279 were white matter hyperintensities (WMHs), 22 were tumor and tumor-like lesions, 26 were arachnoid cyst, 29 were empty sella, 7 were intracranial calcinosis and 2 were craniocerebral trauma. The proportion of no obvious abnormality in 20-29 years group and 30-39 years group were higher than those in other age groups; the detection rates of WMHs in 40-49 years group and 50-59 years group was higher than those in the other age groups; the detection rates of cerebrovascular diseases and empty sella in 50-59 years group were higher than those in the other age groups, and the differences were significant (all P< α/6=0.008). The proportion of no obvious abnormality in fighter pilots and helicopter pilots were higher than those in others; the detection rate of WMHs in air combat/technical personnel was higher than that in others; the detection rate of arachnoid cyst in bomb-transporter pilots was higher than that in fighter pilots, and the differences were significant (all P< α/6=0.008). Most of the abnormal results detected by craniocerebral MRI examination had no significant impact on the conclusion of aeromedical assessment, and those with greater impact were cerebrovascular disease, tumor and tumor-like lesions (both P< α/351=0.001). Conclusions:The MRI examination not only preliminarily explains the basic characteristics of the distribution of craniocerebral diseases among military flying personnel of different ages, aircraft types and posts from the perspective of imaging, but also provides the basis for dynamic follow-up and accurate aeromedical assessment.

Objective:To improve the understanding of craniocerebral diseases and aeromedical assessment from the perspective of imaging by analyzing the results of craniocerebral MRI examination of military flying personnel.Methods:The results of craniocerebral MRI examination were retrospectively analyzed among the military flying personnel who underwent annual physical examination in Air Force Healthcare Center for Special Services Hangzhou from September of 2020 to December of 2021. They were divided into 20-29 years group, 30-39 years group, 40-49 years group, 50-59 years group according to the age, and they were divided into fighter pilots, bomb-transporter pilots, helicopter pilots, air combat/technical personnel according to the aircraft type and post. The detection of craniocerebral MRI examination results of the flying personnel in different age groups and aircraft type and post groups were compared. The influence of abnormal results on the conclusion of aeromedical assessment was analyzed. The α level of statistical analysis was set at 0.05, and the difference was statistically significant with P< α/statistical count for pairwise comparison among groups. Results:Among the craniocerebral MRI examination results of 975 military flying personnel, 473 cases were with no obvious abnormality, 194 were anatomical variation of Willis circle, 27 were congenital brain malformation, 54 were cerebrovascular disease, 279 were white matter hyperintensities (WMHs), 22 were tumor and tumor-like lesions, 26 were arachnoid cyst, 29 were empty sella, 7 were intracranial calcinosis and 2 were craniocerebral trauma. The proportion of no obvious abnormality in 20-29 years group and 30-39 years group were higher than those in other age groups; the detection rates of WMHs in 40-49 years group and 50-59 years group was higher than those in the other age groups; the detection rates of cerebrovascular diseases and empty sella in 50-59 years group were higher than those in the other age groups, and the differences were significant (all P< α/6=0.008). The proportion of no obvious abnormality in fighter pilots and helicopter pilots were higher than those in others; the detection rate of WMHs in air combat/technical personnel was higher than that in others; the detection rate of arachnoid cyst in bomb-transporter pilots was higher than that in fighter pilots, and the differences were significant (all P< α/6=0.008). Most of the abnormal results detected by craniocerebral MRI examination had no significant impact on the conclusion of aeromedical assessment, and those with greater impact were cerebrovascular disease, tumor and tumor-like lesions (both P< α/351=0.001). Conclusions:The MRI examination not only preliminarily explains the basic characteristics of the distribution of craniocerebral diseases among military flying personnel of different ages, aircraft types and posts from the perspective of imaging, but also provides the basis for dynamic follow-up and accurate aeromedical assessment.

L-asparaginase hydrolyzes L-asparagine to produce L-aspartic acid and ammonia. It is widely distributed in microorganisms, plants and serum of some rodents, and has important applications in the pharmaceutical and food industries. However, the poor thermal stability, low catalytic efficiency and low yield hampered the further application of L-asparaginase. In this paper, rational design and 5' untranslated region (5'UTR) design strategies were used to increase the specific enzyme activity and protein expression of L-asparaginase derived from Rhizomucor miehei (RmAsnase). The results showed that among the six mutants constructed through homology modeling combined with sequence alignment, the specific enzyme activity of the mutant A344E was 1.5 times higher than the wild type. Subsequently, a food-safe strain Bacillus subtilis 168/pMA5-A344E was constructed, and the UTR strategy was used for the construction of recombinant strain B. subtilis 168/pMA5 UTR-A344E. The enzyme activity of B. subtilis 168/pMA5 UTR-A344E was 7.2 times higher than that of B. subtilis 168/pMA5-A344E. The recombinant strain B. subtilis 168/pMA5 UTR-A344E was scaled up in 5 L fermenter, and the final yield of L-asparaginase was 489.1 U/mL, showing great potential for industrial application.
Asparaginase/genetics* ; Bacillus subtilis/genetics* ; Industrial Microbiology ; Protein Engineering ; Rhizomucor/enzymology* ; Sequence Alignment

Objective:To explore the influential factors of lumbar inter-vertebral disc degeneration and provide the references on flying personnel′s recruitment, physical checkup for transformation and medical identification.Methods:Lumbar MRI images and relevant data of 458 flying personnel who underwent medical identification in the Second Area of Air Force Healthcare Center for Special Services Hangzhou from March 2018 to May 2019 were retrospectively analyzed. Two thousand two hundred and ninety lumbar inter-vertebral discs of 458 flying personnel were semi quantitatively scored according to the Pfirrmann standard. The sum of five inter-vertebral disc scores of each flying personnel was taken as the lumbar inter-vertebral disc degenerative factor (LIVDDF). This factor was taken as the dependent variable ( y), and the age ( x1), height ( x2), weight ( x3), and flying hours ( x4) of flying personnel were taken as independent variables for linear regression analysis. The subgroup analysis was carried out based on the grouping of aircraft type and flight post. Results:The range of LIVDDF was 5-18, with an average of 10.87±2.83. Total analysis showed that age and weight were the influential factors of lumbar inter-vertebral disc degeneration in flying personnel. The regression equation was y=1.029+0.182 x1+0.038 x3 ( r=0.68, P<0.01). Subgroup analysis on the different aircraft types showed that the influential factor was age in the fighter subgroup. The regression equation was y=2.569+0.225 x1 ( r=0.59, P<0.01). Age and height were the influential factors in the trans-bomber aircraft subgroup. The regression equation was y=-8.848+0.186 x1+7.160 x2( r=0.69, P<0.01). Flying hours was the influential factor in the helicopter subgroup. The regression equation was y=7.823+0.001 x4 ( r=0.65, P<0.01). Subgroup analysis on different flight post showed that the influential factors were age in flying personnel. The regression equation was y=3.176+0.207 x1 ( r=0.65, P<0.01). Age and weight were the influential factors in crew members. The regression equation was y=-0.113+0.171 x1+0.056 x3( r=0.74, P<0.01). Conclusions:Age and weight are the main influential factors of lumbar inter-vertebral disc degeneration in flying personnel, but these factors show different influence on aircraft type and flight post. Due to the small sample size in this study, aviation related factors are not fully included. So other influential factors of lumbar inter-vertebral disc degeneration of flying personnel may exist.

Objective:To explore the influential factors of lumbar inter-vertebral disc degeneration and provide the references on flying personnel′s recruitment, physical checkup for transformation and medical identification.Methods:Lumbar MRI images and relevant data of 458 flying personnel who underwent medical identification in the Second Area of Air Force Healthcare Center for Special Services Hangzhou from March 2018 to May 2019 were retrospectively analyzed. Two thousand two hundred and ninety lumbar inter-vertebral discs of 458 flying personnel were semi quantitatively scored according to the Pfirrmann standard. The sum of five inter-vertebral disc scores of each flying personnel was taken as the lumbar inter-vertebral disc degenerative factor (LIVDDF). This factor was taken as the dependent variable ( y), and the age ( x1), height ( x2), weight ( x3), and flying hours ( x4) of flying personnel were taken as independent variables for linear regression analysis. The subgroup analysis was carried out based on the grouping of aircraft type and flight post. Results:The range of LIVDDF was 5-18, with an average of 10.87±2.83. Total analysis showed that age and weight were the influential factors of lumbar inter-vertebral disc degeneration in flying personnel. The regression equation was y=1.029+0.182 x1+0.038 x3 ( r=0.68, P<0.01). Subgroup analysis on the different aircraft types showed that the influential factor was age in the fighter subgroup. The regression equation was y=2.569+0.225 x1 ( r=0.59, P<0.01). Age and height were the influential factors in the trans-bomber aircraft subgroup. The regression equation was y=-8.848+0.186 x1+7.160 x2( r=0.69, P<0.01). Flying hours was the influential factor in the helicopter subgroup. The regression equation was y=7.823+0.001 x4 ( r=0.65, P<0.01). Subgroup analysis on different flight post showed that the influential factors were age in flying personnel. The regression equation was y=3.176+0.207 x1 ( r=0.65, P<0.01). Age and weight were the influential factors in crew members. The regression equation was y=-0.113+0.171 x1+0.056 x3( r=0.74, P<0.01). Conclusions:Age and weight are the main influential factors of lumbar inter-vertebral disc degeneration in flying personnel, but these factors show different influence on aircraft type and flight post. Due to the small sample size in this study, aviation related factors are not fully included. So other influential factors of lumbar inter-vertebral disc degeneration of flying personnel may exist.

The objective of this study was to prepare a biodegradable poly (DL-lactide) injectable gel of tinidazole. The formulation parameters evaluated in this study included polymer molecular weight, polymer concentration, solvent and drug loading, and orthogonal design was used to optimize the formulation. The preferable formulation was that 30% (w/w) poly(DL-lactide) (MW is 5 700) dissolved in 70% (w/w) N-methyl-2-pyrrolidone with 4%-6% (w/w) tinidazole.
Delayed-Action Preparations ; Drug Carriers ; chemistry ; Gels ; Lactic Acid ; chemistry ; Polyesters ; Polymers ; chemistry ; Technology, Pharmaceutical ; methods ; Tinidazole ; administration & dosage

OBJECTIVETo describe a new noncontact balloon catheter mapping system and to assess the clinical utility of this system for guiding endocardial mapping and ablation of tachycardia.

METHODSFive patients with tachycardia underwent endocardial mapping and radiofrequency ablation using the noncontact balloon catheter mapping system. A 9 French, 64-electrode balloon catheter and a conventional 7 French electrode catheter for mapping and ablation were positioned in the same ventricular chamber. Ventricular three-dimensional geometry was established by the computerized mapping system. Using a boundary element inverse solution, 3360 virtual endocardial electrograms were computerized and used to derive isopotential maps. The earliest endocardial activation site, the exit site and the activation sequence of tachycardia or the critical isthmus of the reentry circuit were identified. Radiofrequency ablation with circular or linear lesion was performed at the target sites guided by the locator system.

RESULTSSix clinical types of tachycardia, 5 of which were ventricular tachycardia and one was concealed fasciculoventricular fiber mediated tachycardia, were induced by programmed stimulation. The mean cycle length of these tachycardias was 336.6 +/- 42.69 msec. The earliest activation site and the exit site of 5 mapped tachycardias were all identified using the system. One type of ventricular tachycardia was hemodynamically unstable and difficult to terminate, and could not be mapped. Among the 6 types of tachycardias, radiofrequency ablation was successful in 4. There was no complication during and after the procedure. During the mean follow-up of 6 months, no tachycardia recurred in the patients with a successful ablation.

CONCLUSIONSThe noncontact mapping system described in this study has advantage over conventional mapping techniques for refractory tachycardia. It is not only helpful for understanding the electrophysiologic mechanism of a complex case, but also suitable for mapping hemodynamically intolerated and nonsustained ventricular tachycardia.

Adult ; Body Surface Potential Mapping ; methods ; Cardiac Catheterization ; Catheter Ablation ; methods ; Catheterization ; methods ; Humans ; Male ; Middle Aged ; Tachycardia, Ventricular ; physiopathology ; surgery