Objective To investigate the role of long non-coding RNA-small nucleolar RNA host gene 3(lncRNA-Snhg3)and its regulatory mechanism in the hepatic glucose metabolism of mice.Methods Adenovirus Snhg3 was over-expressed by the tail vein injection in db/db mice,and then glucose tolerance and pyruvate tolerance were meas-ured.The mRNA expression of mouse liver gluconeogenesis-related genes phosphoenolpyruvate carboxylase(Pepck)and glucose-6-phosphatase(G6pc)and stress-inducing protein 2(Sestrin2,Sesn2,a gene adjacent to Snhg3)were de-tected by RT-qPCR.The dual luciferase reporter assay was used to detect the effect of Snhg3 on the Sesn2 promoter activity in 293T cells.Results Snhg3 over-expression improved glucose tolerance and pyruvate tolerance in db/db mice.Snhg3 over-expression inhibited the mRNA of gluconeogenesis genes of Pepck(P＜0.05)and G6pc(P＜0.05),while promoted the mRNA of Sesn2(P＜0.01).Meanwhile,Snhg3 over-expression promoted Sesn2 promoter activity in 293T cells(P＜0.05).Conclusions Snhg3 improves glucose metabolism in mice by promoting Sestrin2 expression.

Objective:To improve the understanding of craniocerebral diseases and aeromedical assessment from the perspective of imaging by analyzing the results of craniocerebral MRI examination of military flying personnel.Methods:The results of craniocerebral MRI examination were retrospectively analyzed among the military flying personnel who underwent annual physical examination in Air Force Healthcare Center for Special Services Hangzhou from September of 2020 to December of 2021. They were divided into 20-29 years group, 30-39 years group, 40-49 years group, 50-59 years group according to the age, and they were divided into fighter pilots, bomb-transporter pilots, helicopter pilots, air combat/technical personnel according to the aircraft type and post. The detection of craniocerebral MRI examination results of the flying personnel in different age groups and aircraft type and post groups were compared. The influence of abnormal results on the conclusion of aeromedical assessment was analyzed. The α level of statistical analysis was set at 0.05, and the difference was statistically significant with P< α/statistical count for pairwise comparison among groups. Results:Among the craniocerebral MRI examination results of 975 military flying personnel, 473 cases were with no obvious abnormality, 194 were anatomical variation of Willis circle, 27 were congenital brain malformation, 54 were cerebrovascular disease, 279 were white matter hyperintensities (WMHs), 22 were tumor and tumor-like lesions, 26 were arachnoid cyst, 29 were empty sella, 7 were intracranial calcinosis and 2 were craniocerebral trauma. The proportion of no obvious abnormality in 20-29 years group and 30-39 years group were higher than those in other age groups; the detection rates of WMHs in 40-49 years group and 50-59 years group was higher than those in the other age groups; the detection rates of cerebrovascular diseases and empty sella in 50-59 years group were higher than those in the other age groups, and the differences were significant (all P< α/6=0.008). The proportion of no obvious abnormality in fighter pilots and helicopter pilots were higher than those in others; the detection rate of WMHs in air combat/technical personnel was higher than that in others; the detection rate of arachnoid cyst in bomb-transporter pilots was higher than that in fighter pilots, and the differences were significant (all P< α/6=0.008). Most of the abnormal results detected by craniocerebral MRI examination had no significant impact on the conclusion of aeromedical assessment, and those with greater impact were cerebrovascular disease, tumor and tumor-like lesions (both P< α/351=0.001). Conclusions:The MRI examination not only preliminarily explains the basic characteristics of the distribution of craniocerebral diseases among military flying personnel of different ages, aircraft types and posts from the perspective of imaging, but also provides the basis for dynamic follow-up and accurate aeromedical assessment.

Objective:To improve the understanding of craniocerebral diseases and aeromedical assessment from the perspective of imaging by analyzing the results of craniocerebral MRI examination of military flying personnel.Methods:The results of craniocerebral MRI examination were retrospectively analyzed among the military flying personnel who underwent annual physical examination in Air Force Healthcare Center for Special Services Hangzhou from September of 2020 to December of 2021. They were divided into 20-29 years group, 30-39 years group, 40-49 years group, 50-59 years group according to the age, and they were divided into fighter pilots, bomb-transporter pilots, helicopter pilots, air combat/technical personnel according to the aircraft type and post. The detection of craniocerebral MRI examination results of the flying personnel in different age groups and aircraft type and post groups were compared. The influence of abnormal results on the conclusion of aeromedical assessment was analyzed. The α level of statistical analysis was set at 0.05, and the difference was statistically significant with P< α/statistical count for pairwise comparison among groups. Results:Among the craniocerebral MRI examination results of 975 military flying personnel, 473 cases were with no obvious abnormality, 194 were anatomical variation of Willis circle, 27 were congenital brain malformation, 54 were cerebrovascular disease, 279 were white matter hyperintensities (WMHs), 22 were tumor and tumor-like lesions, 26 were arachnoid cyst, 29 were empty sella, 7 were intracranial calcinosis and 2 were craniocerebral trauma. The proportion of no obvious abnormality in 20-29 years group and 30-39 years group were higher than those in other age groups; the detection rates of WMHs in 40-49 years group and 50-59 years group was higher than those in the other age groups; the detection rates of cerebrovascular diseases and empty sella in 50-59 years group were higher than those in the other age groups, and the differences were significant (all P< α/6=0.008). The proportion of no obvious abnormality in fighter pilots and helicopter pilots were higher than those in others; the detection rate of WMHs in air combat/technical personnel was higher than that in others; the detection rate of arachnoid cyst in bomb-transporter pilots was higher than that in fighter pilots, and the differences were significant (all P< α/6=0.008). Most of the abnormal results detected by craniocerebral MRI examination had no significant impact on the conclusion of aeromedical assessment, and those with greater impact were cerebrovascular disease, tumor and tumor-like lesions (both P< α/351=0.001). Conclusions:The MRI examination not only preliminarily explains the basic characteristics of the distribution of craniocerebral diseases among military flying personnel of different ages, aircraft types and posts from the perspective of imaging, but also provides the basis for dynamic follow-up and accurate aeromedical assessment.

Objective:To provide evidence for the physical examination of the subjects in pilot selection, aircraft transferring and normal health check by analyzing the applicability of low-dose chest CT in pulmonary nodule diagnose.Methods:The flying personnel who received the physical examination in Air Force Healthcare Center for Special Services, Hangzhou from December of 2018 to May of 2019 were enrolled in this prospective study. They were randomly divided into observation group and control group by a random number table. The subjects of observation group underwent low dose chest CT, while the subjects of control group underwent routine chest X-ray examination. The detection rate of pulmonary nodules and its influence rate on medical identification were respectively compared between 2 groups. Subgroup analysis based on the size, density, quantity and benign or malignant of pulmonary nodules were also analyzed between 2 groups.Results:A total of 238 flying personnel were enrolled down to each group of 119 subjects. There was no significant difference in age, height, weight, flying hours, flight post and aircraft type between observation group and control group. The detection rate of pulmonary nodules in observation group (41.18%) was significantly higher than that in control group (10.08%) ( P<0.05). The influence rate of pulmonary nodules detection on medical identification in observation group (24.45%) was higher than that in control group (16.67%), but it has no statistical significance ( P>0.05). Subgroup analysis showed that observation group had significantly higher detection rate of pulmonary nodule and ground-glass nodule which had the diameter smaller than 3 mm ( P<0.05). Compared with control group, there was no statistical difference on the detection rate of pulmonary multiple nodules and malignant nodules ( P>0.05). Conclusions:For screening pulmonary nodules in flying personnel, low-dose chest CT is obviously superior to routine chest X-ray examination and can provide better imaging basis for the medical evaluation of flying personnel.

Objective:To provide evidence for the physical examination of the subjects in pilot selection, aircraft transferring and normal health check by analyzing the applicability of low-dose chest CT in pulmonary nodule diagnose.Methods:The flying personnel who received the physical examination in Air Force Healthcare Center for Special Services, Hangzhou from December of 2018 to May of 2019 were enrolled in this prospective study. They were randomly divided into observation group and control group by a random number table. The subjects of observation group underwent low dose chest CT, while the subjects of control group underwent routine chest X-ray examination. The detection rate of pulmonary nodules and its influence rate on medical identification were respectively compared between 2 groups. Subgroup analysis based on the size, density, quantity and benign or malignant of pulmonary nodules were also analyzed between 2 groups.Results:A total of 238 flying personnel were enrolled down to each group of 119 subjects. There was no significant difference in age, height, weight, flying hours, flight post and aircraft type between observation group and control group. The detection rate of pulmonary nodules in observation group (41.18%) was significantly higher than that in control group (10.08%) ( P<0.05). The influence rate of pulmonary nodules detection on medical identification in observation group (24.45%) was higher than that in control group (16.67%), but it has no statistical significance ( P>0.05). Subgroup analysis showed that observation group had significantly higher detection rate of pulmonary nodule and ground-glass nodule which had the diameter smaller than 3 mm ( P<0.05). Compared with control group, there was no statistical difference on the detection rate of pulmonary multiple nodules and malignant nodules ( P>0.05). Conclusions:For screening pulmonary nodules in flying personnel, low-dose chest CT is obviously superior to routine chest X-ray examination and can provide better imaging basis for the medical evaluation of flying personnel.

BACKGROUND:Drug therapy can partly reduce and delay the progress of Alzheimer’s disease, but only 30%with the single drug treatment obtain clinical cure. OBJECTIVE:To study the therapeutic effect of bone marrow mesenchymal stem cel s transplantation for rats with Alzheimer’s disease. METHODS:Amyloidβ-protein was injected into the hippocampus of Sprague-Dawley rats to construct the model of Alzheimer’s disease. And bone marrow stromal stem cel s were transplanted into the hippocampus of the rat models. RESULTS AND CONCLUSION:At 2 weeks after modeling, compared with the control group, the escape latency in the model and experimental groups was significantly longer (P<0.05), which indicating that Alzheimer’s disease models were successful y established. At 4 weeks after cel transplantation, compared with the model group, the average escape latency in the experimental group was significantly decreased, but retention time on the platform quadrant was significantly prolonged (P<0.05). Besides, at 4 weeks after cel transplantation, expression of choline acetyltransferase in the experimental group was significantly higher than that in the model group (P<0.05). In conclusion, bone marrow mesenchymal stem cel s cannot only differentiate and survive in the hippocampus of rats with Alzheimer’s disease, but also improve the learning and memory ability.

BACKGROUND:Drug treatment for senile dementia has unsatisfactory outcomes although to a certain extent it can reduce and delay the progression of Alzheimer’s disease. Stem cel transplantation is a new attempt for the treatment of senile dementia.
OBJECTIVE:To observe the effect of bone marrow mesenchymal stem cel transplantation on the behavior of senile dementia rats.
METHODS: Rat models of senile dementia were made in 20 Sprague-Dawley rats that were given continuous 60-day gavage of aluminium chloride solution. Then, model rats were randomized into model group treated with normal saline injection and experimental group treated with hippocampal injection of bone marrow mesenchymal stem cels, respectively. Another 10 rats undergoing normal feeding served as control group. Learning and memory ability of rats were tested by Morris water maze, and superoxide dismutase activity and malondialdehyde content in brain tissues of rats were measured by colorimetric method at 4 weeks after cel transplantation.
RESULTS AND CONCLUSION:Compared with the model group, the escape latency was shortened and the cross-platform frequency was increased in the experimental group (P < 0.05), and moreover, significantly elevated superoxide dismutase activity and reduced malondialdehyde content in the brain tissues of rats were found in the experimental group (P < 0.05). These findings indicate that bone marrow mesenchymal stem cel transplantation contributes to behavior improvement in senile dementia rats by improving the learning and memory ability.

Objective To determine the optimal dose range of naloxone to enhance the analgesic effect of morphine.Methods One half of a total of 84 adult male Sprague-Dawley rats were randomly assigned into seven groups(6 rats for each group).Rats in group NS received normal saline,and in group M received 6mg/kg of morphine.Different doses of naloxone(1?g/kg,100ng/kg,10ng/kg,1ng/kg and 0.1ng/kg)with 6mg/kg of morphine were given to the rats in group MN1,group MN2,group MN3,group MN4 and group MN5.Pain thresholds were determined at different time points before and after subcutaneous injection of normal saline or morphine or mixture of the drugs(morphine and naloxone).Another 42 rats were randomly assigned into seven groups similar to the above grouping,but the morphine doses for group M and groups MN were changed to 2mg/kg.Acute pain was prodused by an in cision on the hind paw.Then they were given subcutaneous injection of the drugs in different doses as categorized above.Cumulative pain scores were observed within an hour.Results Compared with group NS,the pain thresholds of all the other groups were significantly increased at the time points from 5 minutes to 120 minutes after subcutaneous injection(P0.05).Conclusions Low-dose of naloxone can enhance the analgesic effect of morphine,and the dose range 1ng/kg~100ng/kg may be acceptable.Dose of 1?g/kg naloxone may antagonize the analgesic effect of morphine,while dose of 0.1ng/kg naloxone,perhaps,is too low to show an effect.