To review the current knowledge and beliefs of patients with metabolic dysfunction-associated fatty liver disease (MAFLD) about disease management, including knowledge belief and practice, to find out and analyze the factors that may affect the level of knowledge and beliefs of the patients, including age, occupation, cultural level, family income and social support. We also summarize and propose possible effective interventions for patients with MAFLD from the perspectives of personalized health education and multi-disciplinary team, with the aim of providing references to improve the patients' knowledge, adherence to treatment and quality of life.

To review the current knowledge and beliefs of patients with metabolic dysfunction-associated fatty liver disease (MAFLD) about disease management, including knowledge belief and practice, to find out and analyze the factors that may affect the level of knowledge and beliefs of the patients, including age, occupation, cultural level, family income and social support. We also summarize and propose possible effective interventions for patients with MAFLD from the perspectives of personalized health education and multi-disciplinary team, with the aim of providing references to improve the patients' knowledge, adherence to treatment and quality of life.

Objective:To analyze the epidemiological and etiological characteristics of a death case of meningococcal meningitis in Hengyang city, Hunan Province in 2024.Methods:Epidemiological investigation of the death case was performed, and samples from the patient and close contacts were collected. Following cultivation and isolation, Neisseria meningitidis ( Nm) strains were analyzed by antimicrobial susceptibility testing, pulsed field gel electrophoresis (PFGE), and whole-genome sequencing for analyzing epidemiological and etiological characteristics. Phylogenetic analysis was carried out using core genomic multilocus sequence typing (cgMLST). Results:The case was a 16-year-old high school boarding student with fulminant meningococcal meningitis. He had shock symptoms, and died within 24 h of the onset of symptoms. Six Nm strains were isolated from the patient and his roommates, belonging to two distinct clades. Isolate 144569 from the patient was highly homologous to isolate 144572 from a close contact, both belonging to the highly pathogenic sublineage L44.1 of CC4821. The typical molecular features was C: P1.7-2, 14: F5-101: ST4821 (CC4821). The two strains carried the antimicrobial resistance genes of gyrA-71 and penA-552, indicating reduced susceptibility to quinolone and penicillin, which was with their resistance phenotype. The isolates from four close contacts clustered within the same clade, characterized by the molecular features of B: P1.18-25, 9-18: ST5829 (UA). Conclusions:The death case is caused by Nm serogroup C from highly pathogenic sublineage L44.1 of CC4821. The spread of this isolate has the potential risk of outbreaks of invasive meningococcal disease. It is necessary to enhanced the molecular epidemiological surveillance, particularly focusing on the transmission of multiple serogroups of Nm among adolescents and the increasing exposure risk.

Objective:To investigate the expression of serum circular RNA PUM1 (circPUM1) and microRNA-760 (miR-760) in patients with polycystic ovary syndrome (PCOS) , and their correlation with BMI and biochemical indexes.Methods:A total of 118 patients with PCOS who were diagnosed and treated in Zhengzhou Central Hospital Affiliated to Zhengzhou University from Feb. 2019 to Feb. 2022 were regarded as as the research subjects (PCOS group) . Another 120 women who underwent physical examination in our hospital were regarded as the control group. Real-time quantitative PCR (RT-qPCR) was performed to detect the expression levels of circPUM1 and miR-760 in serum, and to measure serum glucose and lipid metabolism indicators and sex hormone indicators; Pearson method was performed to analyze the correlation between the expression levels of circPUM1 and miR-760, and the correlation between circPUM1 and miR-760 and each index.Results:The expression level of BMI, FBG, FINS, HOMA-IR, TG, estradiol, LH, prolactin and circPUM1 was (23.81±2.85) kg/m 2, (4.87±0.73) ] mmol/L, (8.51±2.18) mIU/L, 1.85±0.69, (1.58±0.57) mmol/L, (182.34±30.47) pmol/L, (7.43±2.05) mIU/ml, (323.45±60.25) mIU/L and 1.05±0.21, while they were (25.24±2.36) kg/m 2, (5.35±0.65) mmol/L, (14.43±4.36) mIU/L, 3.21±1.05, (1.93±0.60) mmol/L, (193.75±38.42) pmol/L, (10.42±2.60) mIU/mL, (372.31±75.44) mIU/L and 1.83±0.45. The expression level of miR-760 was 1.01±0.23 in the control group, while it was 0.77±0.16 in PCOS group. Correlation analysis showed that serum circPUM1 in PCOS patients was negatively correlated with miR-760 ( r=-677, P<0.001) , and serum circPUM1 in PCOS patients was positively correlated with FBG, FINS, HOMA-IR, and TG ( P<0.05) , while was no greatly correlated with other indicators ( P>0.05) , and miR-760 was negatively correlated with FBG, FINS, HOMA-IR, and TG ( P<0.05) , while was no greatly correlated with other indicators ( P>0.05) . Conclusion:The expression level of circPUM1 in serum of patients with PCOS is increased, and the expression level of miR-760 is decreased, which is correlated with glucose and lipid metabolism indicators and can be used to predict the occurrence of metabolic diseases in PCOS patients.

Objective To investigate the infection status of nanobacteria on patients of chronic hepatopathy and hepatocellular carcinoma,and evaluate the clinical value of PCR.Methods In sera of 68 cases of chronic hepatitis B(CHB),56 chronic severe hepatitis B(CSHB),66 cirrhosis of liver(CL)and 23 hepatocellular carcinoma(HCC),nanobacteria were detected by immunohistochemistry stain(IHC),Transmission Electron Microscopy(TEM)and polymerase chain reaction(PCR),compared with 40 healthy people.Results The positive rates of PCR were 27.69%,50.00%,61.29%,52.38% and 5.00% in patients with CHB,CSHB,CL,HCC and normal control respectively(P

Objective:To predict and identify HLA-A * 0201 restricted CD8+ CTL epitopes in Mycobacterium tuberculosis (Mtb) antigen Ag85C, so as to provide evidence for epitope-based study for tuberculosis (TB) vaccine. Methods: The online database SYFPEITHI was applied to predict the potential HLA-A * 0201 restricted epitopes from Ag85C, an antigen of Mycobacterium tuberculosis. T2 cell line was used to assay the affinity between the predicted peptides and HLA-A * 0201 molecules. The specific CTL lines were induced from peripheral blood mononuclear cells (PBMCs) of HLA-A * 0201 positive TB patients and PPD+ healthy donors by peptides with high binding affinity to HLA-A * 0201 molecules. IFN-?production, in vitro proliferation and cytotoxicity of peptide-induced CTL were determined to screen HLA-A * 0201 restricted CD8+ CTL epitopes from those candidates. Results: Fourteen potential epitopes were identified from the SYFPEITHI database. After binding affinity assay, 3 of the 14 peptides (170-178 aa, 317-325 aa, and 144-153 aa) were found to have high binding affinity to HLA-A* 0201 molecules. However, only one peptide (144-153 aa) stimulated its specific CTL to release IFN-y, proliferate in vitro and produce specific cytotoxicity. Conclusion: We have successfully identified a HLA-A * 0201 restricted CD8+ CTL epitope of Mtb Ag85C-FLTREMPAWL( 144-153 aa) , which might be a candidate epitope for TB vaccine designing. Our findings provides a basis for developing novel and effective anti-TB vaccine.