Hepatitis B virus (HBV) infection is a major global public health problem. Persistent HBV infection is prone to develop chronic hepatitis B (CHB), and CHB is closely related to the development of liver fibrosis and hepatocellular carcinoma. High-affinity specific anti-HBs are essential for the control of HBV infection, while the antibody production is closely related to follicular helper T (Tfh) cells. Tfh cells can help B cells differentiate into plasma cells to produce specific antibodies to control virus infection. This article reviews the latest research progress of Tfh cells in HBV infection to provide information of new strategies for the prevention and treatment of HBV.

Objective:To evaluate the clinical efficacy of off-midline closure technique in the treatment of chronic pilonidal sinus.Methods:From July 2014 to July 2018, 59 patients suffered from chronic pilonidal sinus treated by off-midline closure were admitted to the Department of Proctology, Linyi Central Hospital, including 34 males and 25 females. According to the size and scope of the chronic pilonidal sinus, the off-midline flap was designed and sutured.Results:In this group of 59 patients, there were 52 cases of primary healing and 7 cases of incision complications, such as partial incision dehiscence and subcutaneous effusion. After follow-up for 2 years, no recurrence was observed. The scar was not obvious after wound healing. The patient was satisfied with the postoperative appearance.Conclusions:Using the off-midline closure technique to treat chronic pilonidal sinus can deviate from the midline to make the suture tension-free and the gluteal groove elevated. It not only effectively reduce the complication rate in patients, but also has high cure rate, low recurrence rate and does not affect appearance.

Objective To observe the clinical effects of hyperbaric oxygen (HBO) combined with Fufanghuangbai liquid on wound healing after perianal abscess surgery.Methods One hundred and fifty-eight patients with perianal abscess were randomly divided into the observation group and the control group.The patients of the groups were all treated with conventional surgical treatment and external drug application,but the observation group was supplemented with HBO combined with the spraying of Fufanghuangbai liquid on the wound surface.Clinical effects of the 2 groups were closely observed,and wound healing time,granulation growth,seriousness of pain and edema,as well as wound surface effusion were compared between the 2 groups.Results Wound healing time of the observation group (24.91 ± 5.17) days was significantly shorter than that of the control group (31.23 ± 6.01) days,with statistical significance (P ＜ 0.05).Wound edema and granulation growth on the 7th,14th and 21st days after surgery for the observation group were all superior to those of the control group (P ＜ 0.05).Three days after surgery,there were no significant differences in the seriousness of pain and wound surface effusion,when comparisons were made between the 2 groups (P ＜ 0.05).On the 7th and 14th days after surgery,improvement in the above 2 indicators in the treatment group was significantly better than those of the control group,and statistical significance could be seen,when comparisons were made between the 2 groups(P ＜ 0.05).Conclusion HBO combined with Fufanghuangbai liquid could produce good therapeutic effects on the alleviation of wound edema,relief of pain,reduction of wound surface effusion in the treatment of perianal abscess after surgery,and at the same time,it could shorten hospitalization days and improve patient life quality.For this reason,it was worth further clinical popularization.

Objective To observe the clinical effects of hyperbaric oxygen (HBO) combined with Fufanghuangbai liquid on wound healing after perianal abscess surgery.Methods One hundred and fifty-eight patients with perianal abscess were randomly divided into the observation group and the control group.The patients of the groups were all treated with conventional surgical treatment and external drug application,but the observation group was supplemented with HBO combined with the spraying of Fufanghuangbai liquid on the wound surface.Clinical effects of the 2 groups were closely observed,and wound healing time,granulation growth,seriousness of pain and edema,as well as wound surface effusion were compared between the 2 groups.Results Wound healing time of the observation group (24.91 ± 5.17) days was significantly shorter than that of the control group (31.23 ± 6.01) days,with statistical significance (P ＜ 0.05).Wound edema and granulation growth on the 7th,14th and 21st days after surgery for the observation group were all superior to those of the control group (P ＜ 0.05).Three days after surgery,there were no significant differences in the seriousness of pain and wound surface effusion,when comparisons were made between the 2 groups (P ＜ 0.05).On the 7th and 14th days after surgery,improvement in the above 2 indicators in the treatment group was significantly better than those of the control group,and statistical significance could be seen,when comparisons were made between the 2 groups(P ＜ 0.05).Conclusion HBO combined with Fufanghuangbai liquid could produce good therapeutic effects on the alleviation of wound edema,relief of pain,reduction of wound surface effusion in the treatment of perianal abscess after surgery,and at the same time,it could shorten hospitalization days and improve patient life quality.For this reason,it was worth further clinical popularization.