Objective To investigate the medication characteristics of Professor Gu Shizh in the treatment of lumbar disc herniation based on various data mining techniques.Methods The outpatient case records of Professor Gu was collected from January 2018 to September 2024 in the Guoyitang outpatient department of Beijing University of Chinese Medicine(BUCM)and BUCM Famous Elderly TCM Inheritance Research Integrated Platform.Patients with lumbar disc herniation mentioned in Professor Gu Shizhe's book of Zhi Zhen Zhi Yao were also be screened.R Studio 2024.4.3.2 was used for descriptive analysis and Apriori was used for association rule analysis.SPSS Statistics 27.0 was used for clustering analysis,and chiplot website was used for visualize the properties,tastes and meridians of drugs.Cytoscape 3.10.2 was used to build a network of"high frequency drug association",and combined with the TCM theories characteristics and medicine law used by professor Gu Shine in the treatment of lumbar disc herniation were summarized.Results A total of 63 cases were collected,involving 194 prescriptions and 187 kinds of Chinese materia medica.The top 30 drugs with the highest frequency were extracted,and the top 6 drugs were Angelicae Pubescentis Radix,Taxilii Herba,salt Eucommiae Cortex,Glycyrrhizae Radix et Rhizoma Praeparata cum Melle,Chuanxiong Rhizoma,Gentianae Macrophyllae Radix.The main tastes were sweet,bitter and pungent,the main properties were warm and neutral.The main meridians were liver,spleen,lung,kidney and stomach meridians.Three core medicinal combinations were obtained by clustering analysis,including Duhuo Jisheng Decoction,Gegeng Decoction,Zhijing Powder and Xuduan Pill.Conclusion The focus of Professor Gu's treatment for lumbar disc herniation is to nourish the liver and kidneys,warm and replenish qi and blood,reflecting the characteristics of"qi and blood are in harmony,essence and blood are equally emphasized"in medication

Objective To investigate the medication characteristics of Professor Gu Shizh in the treatment of lumbar disc herniation based on various data mining techniques.Methods The outpatient case records of Professor Gu was collected from January 2018 to September 2024 in the Guoyitang outpatient department of Beijing University of Chinese Medicine(BUCM)and BUCM Famous Elderly TCM Inheritance Research Integrated Platform.Patients with lumbar disc herniation mentioned in Professor Gu Shizhe's book of Zhi Zhen Zhi Yao were also be screened.R Studio 2024.4.3.2 was used for descriptive analysis and Apriori was used for association rule analysis.SPSS Statistics 27.0 was used for clustering analysis,and chiplot website was used for visualize the properties,tastes and meridians of drugs.Cytoscape 3.10.2 was used to build a network of"high frequency drug association",and combined with the TCM theories characteristics and medicine law used by professor Gu Shine in the treatment of lumbar disc herniation were summarized.Results A total of 63 cases were collected,involving 194 prescriptions and 187 kinds of Chinese materia medica.The top 30 drugs with the highest frequency were extracted,and the top 6 drugs were Angelicae Pubescentis Radix,Taxilii Herba,salt Eucommiae Cortex,Glycyrrhizae Radix et Rhizoma Praeparata cum Melle,Chuanxiong Rhizoma,Gentianae Macrophyllae Radix.The main tastes were sweet,bitter and pungent,the main properties were warm and neutral.The main meridians were liver,spleen,lung,kidney and stomach meridians.Three core medicinal combinations were obtained by clustering analysis,including Duhuo Jisheng Decoction,Gegeng Decoction,Zhijing Powder and Xuduan Pill.Conclusion The focus of Professor Gu's treatment for lumbar disc herniation is to nourish the liver and kidneys,warm and replenish qi and blood,reflecting the characteristics of"qi and blood are in harmony,essence and blood are equally emphasized"in medication

Objectives To investigate the changes of erythropoietin(EPO)expression in rats after focal cerebral ischemia/reperfusion injury.Methods Male Sprague-Dawley rats were randomly divided into normal,sham,cerebral ischemic/reperfusion(CIR)groups.Middle cerebral artery occlusion(MACO)model was established by Longa's method,and reperfusion was followed 2 hours after occlusion in CIR group.The rats' brain neurological deficit scores were evaluated at 24 h,48 h,72 h and 96 h after reperfusion.The protein expression of EPO was determined by immunohistochemistry staining and Western blotting in each time points.Results The rats' brain neurological deficit scores at 48 h,72 h and 96 h were significantly increased(3.40±0.32,3.60±0.17,3.70±0.21,all P<0.05)compared with those at 24 h(3.00±0.22)after reperfusion in CIR group.The results of immunohistochemistry staining and Western blotting showed that the positive expression of EPO proteins in rats started at 24 h(0.36±0.05,140.20±0.30)after cerebral ischemic/reperfusion injury,increased significantly at 48 h(1.09±0.10,145.40±0.16),reached the peak at 72 h(1.29±0.09,156.23±0.12),began to decline at 96 h(0.98±0.04,141.56±0.36).Conclusions Cerebral ischemia and reperfusion injury can induce increased expression of EPO protein,which suggests that EPO may have protective effect on nerve cells under the condition of ischemia and reperfusion.