Background The Inner Mongolia Autonomous Region is a vast area with a wide array of ecological environments, resulting in considerable regional variations in air pollution characteristics. Current research is limited by a scarcity of systematic, region-wide studies and risk assessments. Objective To assess the health risks associated with inhalation exposure to nine heavy metal and metalloid elements in atmospheric fine particulate matter (PM_2.5) for the population of the Inner Mongolia Autonomous Region. Methods From the 10th to the 16th of each month throughout 2023, atmospheric PM_2.5 samples were collected at designated monitoring sites in 12 leagues (cities) across the Inner Mongolia Autonomous Region to analyze the characteristics and trends in concentration. The health risk assessment model developed by the United States Environmental Protection Agency was employed to evaluate both the non-carcinogenic and carcinogenic risks associated with the heavy metal elements beryllium (Be), cadmium (Cd), chromium (Cr), hydrargyrum (Hg), plumbum (Pb), manganese (Mn), and nickel (Ni) and the metalloid elements stibium (Sb) and arsenic (As). Results In 2023, a total of 1206 valid PM_2.5 samples were analyzed from the Inner Mongolia Autonomous Region. The annual average PM_2.5 concentrations in Inner Mongolia, as well as in the cities of Ordos, Wuhai, and the Alxa League, all exceeded the national limit of 35 μg·m⁻³. The PM_2.5 concentrations across the leagues (cities) exhibited a clear seasonal variation, peaking in winter, followed by spring, and reaching a minimum in summer and autumn. The median PM_2.5 concentration in Ordos throughout the year was higher than that in the other leagues (cities) of the region. Concerning health risks, the non-carcinogenic risks associated with the 9 elements detected in each league (city) were all below 1, indicating acceptable levels. However, the non-carcinogenic risk values for Mn, Cr, and As were relatively high; specifically, Ordos City displayed the highest non-carcinogenic risk values for Mn (7.74×10⁻¹) and Cr (2.97×10⁻²), while Chifeng City recorded the highest value for As (2.34×10⁻³). The carcinogenic risk values for As, Cd, Cr, and Ni fell within the range of 1×10⁻⁹ to 1×10⁻⁵, representing an acceptable level. The health risks varied with age, and the elderly residents faced the highest non-carcinogenic and carcinogenic health risks. Conclusion While the carcinogenic and non-carcinogenic risks from heavy metals and metalloids within atmospheric PM_2.5 in the Inner Mongolia Autonomous Region are generally within acceptable limits, the potential risks that Mn, As, and Cr pose to the health of elderly people warrants particular attention, and Ordos and Chifeng cities exhibit notably higher health risks among other areas. It is recommended that regular, long-term monitoring be conducted, taking into account the specific conditions at monitoring sites across each league (city), and that targeted air quality management strategies be implemented to protect public health.

Objective:To investigate the molecular mechanism of miR-886-5p targeting BCL-2-associated X protein (BAX) to inhibit the proliferation, migration, and invasion of liver cancer cells.Methods:mRNA expression data of HCC patients were obtained from the Starbase database, including 370 liver cancer samples and 50 normal liver tissue samples adjacent to the cancer. Analyze the expression of miR-886-5p in the previously obtained data and investigate the relationship between miR-886-5p and BAX in liver cancer samples. After transfection of the corresponding plasmids into Huh7 and HepG2 cells, the following groups were established. Analyze the interaction between miR-886-5p and BAX in vitro, detect the protein expression by Western blotting, and verify the targeting relationship between the two by dual luciferase reporter gene assay.Results:Starbase database analysis found that the standardized expression level of miR-886-5p in 370 liver cancer samples was lower than that in normal liver tissue samples (0.12±0.07 vs. 0.73±0.27, t=-15.71, P<0.001), and the expression level of miR-886-5p was positively correlated with the expression level of BAX ( r=0.152, P=0.003). qRT-PCR analysis showed that the expression level of miR-886-5p in HL-7702 cells was higher than that in Huh7 (4.57±0.06 vs. 1.61±0.40, t=32.48) and HepG2 (4.57±0.06 vs. 1.03±0.13, t=143.9), and the expression level of BAX in HL-7702 cells was higher than that in Huh7 (4.01±0.12 vs. 1.28±0.09, t=82.20) and HepG2 (4.01±0.12 vs. 1.30±0.11, t=80.76), the differences were statistically significant (all P<0.001). The proliferation, migration, and invasion abilities of Huh7 and HepG2 cells decreased after transfection with miR-886-5p mimics, while the expression levels of BAX at the mRNA and protein levels increased. However, after inhibiting the expression of miR-886-5p, the above indicators of cells were the opposite, and the dif-ferences were statistically significant (all P<0.05). The viability, EdU positivity rate, cell migration rate, and number of transmembrane cells in the miR-886-5p+ BAX group were lower than those in the BAX group, and the relative expression levels of miR-886-5p, BAX mRNA, and BAX protein were higher than those in the BAX group. However, the above indicators in the Sponge+ BAX group showed opposite trends, and all differences were statistically significant (all P<0.05). There was a targeted binding site between miR-886-5p and BAX. Conclusion:Both miR-886-5p and BAX are downregulated in liver cancer, and miR-886-5p inhibits the proliferation, migration, and invasion of liver cancer cells by targeting BAX.

Objective To explore the quantitative electroencephalography(qEEG)characteristics of the prefrontal cortex in patients with mild cognitive impairment(MCI)during digital screening tasks for MCI screening.Methods A total of 592 MCI patients(MCI group)and 317 normal cognitively elderly individuals(control group)were recruited from 40 communities in Nanjing,Jiangsu Province,from July to August,2024.All participants were as-sessed using Montreal Cognitive Assessment-Beijing Version(MoCA-BJ).Prefrontal EEG data were collected using a portable EEG device,and power spectral analysis was performed via Fast Fourier Transform.An XG-Boost algorithm was employed to construct an MCI identification model based on qEEG power features,and the model's performance was evaluated using receiver operating characteristic(ROC)curve.Results Compared with the control group,prefrontal δ,α,and β band power increased during screening tasks in MCI group(P<0.05);δ power was negatively correlated with MoCA-BJ total scores,and visuospatial/executive func-tion,attention and delayed recall scores(r=-0.269,-0.169,-0.133,-0.171,P<0.001);α power was negative-ly correlated with MoCA-BJ total scores,attention and delayed recall scores(r=-0.113,-0.075,-0.091,P<0.05).The XGBoost model based on δ and α power was excellent in MCI identification,with an area under the curve of 0.91,accuracy of 0.81,precision of 0.89,F1 score of 0.84,recall of 0.80,and specificity of 0.81.Conclusion MCI patients exhibit increased power in the prefrontal δ and α frequency bands during digital screening tasks,which is associated with cognitive decline.An XGBoost model based on qEEG power features can enable early prediction of MCI.

Objective To explore the quantitative electroencephalography(qEEG)characteristics of the prefrontal cortex in patients with mild cognitive impairment(MCI)during digital screening tasks for MCI screening.Methods A total of 592 MCI patients(MCI group)and 317 normal cognitively elderly individuals(control group)were recruited from 40 communities in Nanjing,Jiangsu Province,from July to August,2024.All participants were as-sessed using Montreal Cognitive Assessment-Beijing Version(MoCA-BJ).Prefrontal EEG data were collected using a portable EEG device,and power spectral analysis was performed via Fast Fourier Transform.An XG-Boost algorithm was employed to construct an MCI identification model based on qEEG power features,and the model's performance was evaluated using receiver operating characteristic(ROC)curve.Results Compared with the control group,prefrontal δ,α,and β band power increased during screening tasks in MCI group(P<0.05);δ power was negatively correlated with MoCA-BJ total scores,and visuospatial/executive func-tion,attention and delayed recall scores(r=-0.269,-0.169,-0.133,-0.171,P<0.001);α power was negative-ly correlated with MoCA-BJ total scores,attention and delayed recall scores(r=-0.113,-0.075,-0.091,P<0.05).The XGBoost model based on δ and α power was excellent in MCI identification,with an area under the curve of 0.91,accuracy of 0.81,precision of 0.89,F1 score of 0.84,recall of 0.80,and specificity of 0.81.Conclusion MCI patients exhibit increased power in the prefrontal δ and α frequency bands during digital screening tasks,which is associated with cognitive decline.An XGBoost model based on qEEG power features can enable early prediction of MCI.

Objective:To investigate the molecular mechanism of miR-886-5p targeting BCL-2-associated X protein (BAX) to inhibit the proliferation, migration, and invasion of liver cancer cells.Methods:mRNA expression data of HCC patients were obtained from the Starbase database, including 370 liver cancer samples and 50 normal liver tissue samples adjacent to the cancer. Analyze the expression of miR-886-5p in the previously obtained data and investigate the relationship between miR-886-5p and BAX in liver cancer samples. After transfection of the corresponding plasmids into Huh7 and HepG2 cells, the following groups were established. Analyze the interaction between miR-886-5p and BAX in vitro, detect the protein expression by Western blotting, and verify the targeting relationship between the two by dual luciferase reporter gene assay.Results:Starbase database analysis found that the standardized expression level of miR-886-5p in 370 liver cancer samples was lower than that in normal liver tissue samples (0.12±0.07 vs. 0.73±0.27, t=-15.71, P<0.001), and the expression level of miR-886-5p was positively correlated with the expression level of BAX ( r=0.152, P=0.003). qRT-PCR analysis showed that the expression level of miR-886-5p in HL-7702 cells was higher than that in Huh7 (4.57±0.06 vs. 1.61±0.40, t=32.48) and HepG2 (4.57±0.06 vs. 1.03±0.13, t=143.9), and the expression level of BAX in HL-7702 cells was higher than that in Huh7 (4.01±0.12 vs. 1.28±0.09, t=82.20) and HepG2 (4.01±0.12 vs. 1.30±0.11, t=80.76), the differences were statistically significant (all P<0.001). The proliferation, migration, and invasion abilities of Huh7 and HepG2 cells decreased after transfection with miR-886-5p mimics, while the expression levels of BAX at the mRNA and protein levels increased. However, after inhibiting the expression of miR-886-5p, the above indicators of cells were the opposite, and the dif-ferences were statistically significant (all P<0.05). The viability, EdU positivity rate, cell migration rate, and number of transmembrane cells in the miR-886-5p+ BAX group were lower than those in the BAX group, and the relative expression levels of miR-886-5p, BAX mRNA, and BAX protein were higher than those in the BAX group. However, the above indicators in the Sponge+ BAX group showed opposite trends, and all differences were statistically significant (all P<0.05). There was a targeted binding site between miR-886-5p and BAX. Conclusion:Both miR-886-5p and BAX are downregulated in liver cancer, and miR-886-5p inhibits the proliferation, migration, and invasion of liver cancer cells by targeting BAX.

Objective:To investigate the effect of hUCMSC-exos on the expression levels of HDAC in different isotypes of RA FLSs, and to elucidate the possible mechanism of hUCMSC-exos on the apoptosis of RA FLSs by regulating HDAC.Methods:hUCMSC and hUCMSC-Exos were isolated and identified. RT-qPCR was used to detect the changes in HDAC mRNA expression levels in FLSs after hUCMSC-Exos intervention, and the most affected HDAC types were identified. Western blot was used to detect the levels of FLS HDAC1 protein and the expression levels of NF-κB p65 and phospho-NF-κB p65 (Ser 536) in the blank control group, hUCMSC group, hUCMSC-Exos group, Trichostatin A (TSA) group and HDAC1 Inhibitor (Pyroxamide) group. To investigate the effects of hUCMSC-Exos on HDAC expression and NF-κB activity in FLSs. Flow cytometry was used to detect the effect of hUCMSC-Exos on the apoptosis of FLSs. ELISA was used to detect the effects of hUCMSC-Exos on the secretion of TNF-α, IL-6, IL-1β and IL-8 by FLSs. Flow cytometry and ELISA were used to detect the apoptosis level and pro-inflammatory cytokine secretion level of RA FLSs in the blank control group, NF-κB Inhibitor (pyrrolidine dithiocarbamate (PDTC) group, hUCMSC-Exos group and PDTC+hUCMSC-Exos co-intervention group. Whether inhibition of NF-κB affects the regulatory effect of hUCMSC-Exos on RA FLSs was further explored. All experimental data conforming to the normal distribution were compared by one-way ANOVA. LSD- t test was used for pin-group comparison, and independent sample t test was used for two-sample comparison. Results:Cultured primary hUCMSC were adherently grown spindle-shaped cells, and hUCMSC-Exos were saucer-shaped membranous vesicles, both of which met the identification criteria. hUCMSC-Exos reduced the expression level of HDCA1 mRNA [(0.932±0.091), t=2.19, P<0.001] and protein [(0.204±0.012), t=8.28, P<0.001] in RA FLSs, and the inhibitory effect was stronger than that of hUCMSC ( t=1.09, P=0.009) and HDAC1 ( t=11.29, P=0.013) Inhibitor. hUCMSC-Exos increased the apoptosis rate of RA FLSs [(48.68±0.84)%, t=12.33, P<0.001]. hUCMSC-Exos reduced the secretion levels of TNF-α [(29.6±1.0)pg/ml, t=10.78, P<0.001], IL-6 [(20.1±0.7)pg/ml, t=7.96, P<0.001], IL-1β [(9.28±0.23)pg/ml, t=6.14, P<0.001] and IL-8 [(108.0±3.8)pg/ml, t=1.21, P<0.001] in the supernatant of RA FLSs. hUCMSC-Exos reduced the expression level of p-NF-κB-p65/NF-κB-p65 in RA FLSs(0.351±0.024, t=17.67, P<0.001), and its inhibitory effect was stronger than that of hUCMSC (0.515±0.064, t=8.07, P=0.009) and HDAC1 inhibitor(0.411±0.033, t=2.44, P=0.04). After use of NF-κB inhibitors, hUCMSC-Exos weakened the promotion of apoptosis of RA FLSs [(29.0±0.5)%, t=10.63, P<0.001] and weakened the inhibitory effect of IL-8 secretion in the supernatant of RA FLSs [(125.5±3.2)pg/ml, t=2.63, P=0.002]. Conclusion:hUCMSC-Exos can mimic maternal cells to effectively inhibit the aberrant expression of HDAC1 in RA FLSs. hUCMSC-Exos may affect the apoptosis of RA FLSs and the secretion of pro-inflammatory cytokines by inhibiting the HDAC1/NF-κB pathway.

Objective:To investigate the effect of circular RNA-Hsa-0101216 (Hsa-circ-0101216) targeting microRNA-142-3p (miR-142-3p) on the proliferation,cloning,migration and invasion of pancreatic cancer cells, and explore its molecular mechanism.Methods:The differentially expressed miRNAs in pancreatic cancer were analyzed and screened with GEO database and the circRNA-miRNA network was constructed. The expression of Hsa-circ-0101216 miRNA and miR-142-3p in 5 strains of pancreatic cancer cells (BxPC-3, PANC1, MIA PaCa-2, Capan-2, CFPAC-1) and normal pancreatic duct epithelial cells (HPNE) was detected by quantitative real time PCR. The PANC1 and Capan-2 pancreatic cancer cells were divided into the Hsa-circ-0101216 small interference RNA transfection group (si-circRNA group), the senseless negative sequence siRNA transfection group (si-NC group), and the normal blank control (NC group); the miR-142-3p overexpression lentiviral vector transfection group (miR-142-3p mimic group), the empty vector transfection group (miR-CON group), the co transfection of Hsa-circ-0101216 siRNA and miR-142-3p mimic group (si-Hsa circ0101216+mimic miR-142-3p group), co transfection of Hsa-circ-0101216 siRNA and miR-142-3p mimic negative control sequence group (si-Hsa-circ-010126+mimic miR-142-3p-NC group). The changes of cell proliferation, cloning, migration and invasion were detected by CCK-8, EdU proliferative staining, plate cloning, cell scratch and Transwell assay. Detect the expression of ERK1 and ERK1 and ERK2 protein expression was measured by Western blotting, and the targeting relationship between Hsa-cic-0101216 and miR-142-3p was verified by dual luciferase reporter gene method.Results:The expression of miR-142-3p was significantly down-regulated by GEO analysis, and the Hsa-circ-0101216-miR-142-3p regulatory network was successfully constructed. The mRNA expression levels of Hsa circ0101216 in BxPC-3, PANC1, MIA PaCa-2, Capan-2, and CFPAC-1 cells were 5.64±0.34, 5.93±0.40, 5.66±0.14, 5.63±0.33, and 5.70±0.50, respectively, which were significantly higher than those in HPNE cells (1.27±0.06); the expression levels of miR-142-3p were 1.43±0.12, 1.20±0.09, 1.60±0.04, 1.16±0.25, and 1.42±0.11, respectively, which were significantly lower than those of HPNE cells (4.69±0.22), and all the differences were statistically significant (all P value <0.001). Among them, the expression level of Hsa-circ-0101216 mRNA in PANC1 cells was the highest, and the expression level in Capan 2 cells was the lowest. Compared with the NC group and si-NC group, the si-circRNA group showed a significant decrease in the absorbance value at 450 nm at 24, 48, and 72 hours ( A450 value), EdU positivity rate, cell clone number, migration rate, and transmembrane cell number of PANC1 and Capan-2 cells; the A450 value at the above time points, EdU positivity rate, cell clone number, migration rate, and transmembrane cell number of PANC1 and Capan-2 cells in the miR-142-3p mimic group were significantly lower than those in the NC group and miR-CON group. The A450 value, EdU positivity rate, cell clone number, migration rate, transmembrane cell number, ERK1 and ERK2 protein expression levels of PANC1 and Capan-2 cells in the si-Hsa-circ-0101216+mimic-miR-142-3p group were significantly reduced compared to the si-Hsa-circ-0101216+mimic miR-142-3p-NC group, and the differences were statistically significant (all P<0.001).The luciferase activity of PANC1 and Capan-2 cells in co transfected with wild-type (WT) Hsa-circ-0101216 and miR-142-3p mimic groups was significantly lower than that of the miR-CON+WT group (0.92±0.11 vs 2.33±0.21, 0.89±0.08 vs 2.30±0.17), and the differences were statistically significant (all P value <0.001), but there was no statistically significant difference on luciferase activity of PANC1 and Capan-2 cells between the co transfected mutant (MUT) Hsa-circ-0101216 and miR-142-3p mimic groups and the miR-CON+MUT group. Conclusions:Hsa-circ-0101216 is overexpressed in pancreatic cancer cells, while miR-142-3p is poorly expressed; Hsa-circ-0101216 can promote the proliferation, cloning, migration and invasion of pancreatic cancer cells by targeting miR-142-3p.

Objective:To investigate the effect of circular RNA-Hsa-0101216 (Hsa-circ-0101216) targeting microRNA-142-3p (miR-142-3p) on the proliferation,cloning,migration and invasion of pancreatic cancer cells, and explore its molecular mechanism.Methods:The differentially expressed miRNAs in pancreatic cancer were analyzed and screened with GEO database and the circRNA-miRNA network was constructed. The expression of Hsa-circ-0101216 miRNA and miR-142-3p in 5 strains of pancreatic cancer cells (BxPC-3, PANC1, MIA PaCa-2, Capan-2, CFPAC-1) and normal pancreatic duct epithelial cells (HPNE) was detected by quantitative real time PCR. The PANC1 and Capan-2 pancreatic cancer cells were divided into the Hsa-circ-0101216 small interference RNA transfection group (si-circRNA group), the senseless negative sequence siRNA transfection group (si-NC group), and the normal blank control (NC group); the miR-142-3p overexpression lentiviral vector transfection group (miR-142-3p mimic group), the empty vector transfection group (miR-CON group), the co transfection of Hsa-circ-0101216 siRNA and miR-142-3p mimic group (si-Hsa circ0101216+mimic miR-142-3p group), co transfection of Hsa-circ-0101216 siRNA and miR-142-3p mimic negative control sequence group (si-Hsa-circ-010126+mimic miR-142-3p-NC group). The changes of cell proliferation, cloning, migration and invasion were detected by CCK-8, EdU proliferative staining, plate cloning, cell scratch and Transwell assay. Detect the expression of ERK1 and ERK1 and ERK2 protein expression was measured by Western blotting, and the targeting relationship between Hsa-cic-0101216 and miR-142-3p was verified by dual luciferase reporter gene method.Results:The expression of miR-142-3p was significantly down-regulated by GEO analysis, and the Hsa-circ-0101216-miR-142-3p regulatory network was successfully constructed. The mRNA expression levels of Hsa circ0101216 in BxPC-3, PANC1, MIA PaCa-2, Capan-2, and CFPAC-1 cells were 5.64±0.34, 5.93±0.40, 5.66±0.14, 5.63±0.33, and 5.70±0.50, respectively, which were significantly higher than those in HPNE cells (1.27±0.06); the expression levels of miR-142-3p were 1.43±0.12, 1.20±0.09, 1.60±0.04, 1.16±0.25, and 1.42±0.11, respectively, which were significantly lower than those of HPNE cells (4.69±0.22), and all the differences were statistically significant (all P value <0.001). Among them, the expression level of Hsa-circ-0101216 mRNA in PANC1 cells was the highest, and the expression level in Capan 2 cells was the lowest. Compared with the NC group and si-NC group, the si-circRNA group showed a significant decrease in the absorbance value at 450 nm at 24, 48, and 72 hours ( A450 value), EdU positivity rate, cell clone number, migration rate, and transmembrane cell number of PANC1 and Capan-2 cells; the A450 value at the above time points, EdU positivity rate, cell clone number, migration rate, and transmembrane cell number of PANC1 and Capan-2 cells in the miR-142-3p mimic group were significantly lower than those in the NC group and miR-CON group. The A450 value, EdU positivity rate, cell clone number, migration rate, transmembrane cell number, ERK1 and ERK2 protein expression levels of PANC1 and Capan-2 cells in the si-Hsa-circ-0101216+mimic-miR-142-3p group were significantly reduced compared to the si-Hsa-circ-0101216+mimic miR-142-3p-NC group, and the differences were statistically significant (all P<0.001).The luciferase activity of PANC1 and Capan-2 cells in co transfected with wild-type (WT) Hsa-circ-0101216 and miR-142-3p mimic groups was significantly lower than that of the miR-CON+WT group (0.92±0.11 vs 2.33±0.21, 0.89±0.08 vs 2.30±0.17), and the differences were statistically significant (all P value <0.001), but there was no statistically significant difference on luciferase activity of PANC1 and Capan-2 cells between the co transfected mutant (MUT) Hsa-circ-0101216 and miR-142-3p mimic groups and the miR-CON+MUT group. Conclusions:Hsa-circ-0101216 is overexpressed in pancreatic cancer cells, while miR-142-3p is poorly expressed; Hsa-circ-0101216 can promote the proliferation, cloning, migration and invasion of pancreatic cancer cells by targeting miR-142-3p.

Objective:To analyze the material basis, target and pathway of Chebulae fructus and Margarita in Mongolian medicine Garidi-13 Pill on stroke with the method of network pharmacology and molecular docking technology, so as to better understand its "detoxification" mechanism. Methods:TCMIP and BATMAN-TCM were used to predict the target of Chebulae fructus and Margarita composition, and GeneCards were used to search for the target of stroke. The overlapping targets of the two platforms were imported into the Metascape software for GO biological analysis and KEGG pathway analysis. The molecular docking of key molecules and targets was carried out with LEDOCK. Results:A total of 22 active components were collected and 217 targets related to stroke were predicted. Among them, 1-O-galloyl-glucose, cuprum, ellagicacid, arjungenin and corilagin were the key substances playing the role of "detoxification" of the Chebulae fructus and Margarita; IL6, TNF, HSP90AA1, PTGS2, CASP3, NR1I2, VKORC1 and ATP1A1 were the key targets playing the role of "detoxification" . These targets were significantly enriched in cell response, humoral level regulation, hemostasis, response to steroid hormones, steroid metabolism, coagulation and other biological processes, as well as nitrogen metabolism, IL-17 signaling pathway and other pathways. Molecular docking verified the accuracy of previous prediction results from computer simulation level. Conclusion:The process of Chebulae fructus and Margarita intervening strok is closely related to the elimination of harmful metabolites and calmingthe inflammatory reaction, which was not only consistent with the modern medicine on the pathological process of stroke, but also consistent with the interpretation of "evil and poison" with Mongolian medicine theory.

Objective:Dysmenorrhea is the common gynecological problem among adolescent girls and women of reproductive age. This study aim to develop a dysmenorrhea Quality of Life (QoL) scale based on Traditional Chinese Medicine (TCM) theory.Methods:We conducted focus group discussions and in-depth interviews with TCM gynecologists and patients, and adapted items from previously published scales. We generated an initial pool of 41 items with 8 domains. Delphi method was used to item preliminary selection. Then, we administered the items to a sample of adolescent girls ( n=200), and the distribution of survey items, discrete trend, factor analysis, correlation coefficient, Cronbach’s α coefficient were used to select items. Results:After two rounds of Delphi, a total of thirty items were included in the dysmenorrhea QoL scale. The expert positive coefficient were 100% and 83.3% with high motivation. The authoritative coefficient were greater than 0.7, the results showing authoritative and reliable. The Kendall’s coefficient of concordance W was 0.333 ( χ2=128.271, P<0.001). In sample analysis, the items were deleted when they met more than two standards. The final scale retained 20 items, covering 8 dimensions. Conclusions:The methods for selection of dysmenorrhea QoL scale based on TCM theory were preferable. Given the paucity of research in this area, this new dysmenorrhea QoL scale may provide opportunities for the patient-reported outcome (PRO) evaluation in the field of TCM.