Objective:To study influence of Connect-Introduce-Communicate-Ask-Respond-Exit(CICARE)communication mode on patients with acute heart failure(AHF).Methods:A total of 156 AHF patients treated in our hospital were randomly and equally divided into routine nursing group and CICARE group(received CICARE communication mode based on routine nursing group).Both groups were intervened for one month.General clinical data,hospitalization indexes,scores of Hamilton rating scale for depression(HAMD),Hamilton rating scale for anxiety(HAMA),general self-efficacy scale(GSES)and quality of life scale(QOL-35)before and after inter-vention and incidence of complications were compared between two groups.Results:Compared with routine nursing group,there were significant reductions in first aid time[(42.06±9.77)s vs.(20.27±3.77)s],visit time[(95.67±23.18)min vs.(50.07±11.21)min],admission time[(3.22±0.36)min vs.(2.60±0.67)min]and hospital stay[(22.33±4.82)d vs.(14.13±2.42)d];and scores of HAMD[(14.02±1.42)scores vs.(6.04± 1.57)scores]and HAMA[(13.24±2.07)scores vs.(7.16±2.17)scores]after intervention,and significant rise in scores of GSES[(14.25±3.14)scores vs.(32.03±6.06)scores]and QOL-35[(70.67±5.75)scores vs.(86.93±5.51)scores]in CICARE group after intervention(P=0.001 all).Incidence rate of complications in CICARE group was significantly lower than that of CICARE group(5.13％vs.20.51％,P=0.004).Conclusion:CICARE communication mode can significantly alleviate adverse emotions and improve self-efficacy in patients with acute heart failure.

AIM To establish a UPLC-MS/MS method for the simultaneous content determination of gallic acid,protocatechuic acid,neomangiferin,catechin,caffeic acid,mangiferin,isomangiferin,albiflorin,paeoniflorin,vitexin,liquiritin,scutellarin,baicalin,liquiritigenin,timosaponin BⅡ,quercetin,wogonoside,benzoylpaeoniflorin,isoliquiritigenin,honokiol,magnolol,norarecaidine,arecaidine,arecoline,epicatechin,baicalein,glycyrrhizinate and wogonin in Dayuan Drink.METHODS The analysis was performed on a 35℃thermostatic Syncronis C18 column(100 mm×2.1 mm,1.7 μm),with the mobile phase comprising of 0.1%formic acid-acetonitrile flowing at 0.3 mL/min in a gradient elution manner,and electron spray inoization source was adopted in positive and negative ion scanning with select reaction monitoring mode.RESULTS Twenty-eight constituents showed good linear relationships within their own ranges(R2≥0.991 0),whose average recoveries were 95.60%-103.53%with the RSDs of 0.60%-5.45%.CONCLUSION This rapid,simple,selective,accurate and reliable method can be used for the quality control of Dayuan Drink.

OBJECTIVE: To investigate the effect of Baicalin on the proliferation and pyroptosis of diffuse large B-cell lymphoma cell line DB and its mechanism. METHODS: DB cells were treated with baicalin at different concentrations (0, 5, 10, 20, 40 μmol/L). Cell proliferation was detected by CCK-8 assay and half maximal inhibitory concentration (IC₅₀) was calculated. The morphology of pyroptosis was observed under an inverted microscope, the integrity of the cell membrane was verified by LDH content release assay, and the expressions of pyroptosis-related mRNA and protein (NLRP3, GSDMD, GSDME, N-GSDMD, N-GSDME) were detected by real-time fluorescence quantitative PCR and Western blot. In order to further clarify the relationship between baicalin-induced pyroptosis and ROS production in DB cells, DB cells were divided into control group, baicalin group, NAC group and NAC combined with baicalin group. DB cells in the NAC group were pretreated with ROS inhibitor N-acetylcysteine (NAC) 2 mmol/L for 2 h. Baicalin was added to the combined treatment group after pretreatment, and the content of reactive oxygen species (ROS) in the cells was detected by DCFH-DA method after 48 hours of culture. RESULTS: Baicalin inhibited the proliferation of DB cells in a dose-dependent manner (r=-0.99), and the IC₅₀ was 20.56 μmol/L at 48 h. The morphological changes of pyroptosis in DB cells were observed under inverted microscope. Compared with the control group, the release of LDH in the baicalin group was significantly increased (P<0.01), indicating the loss of cell membrane integrity. Baicalin dose-dependently increased the expression levels of NLRP3, N-GSDMD, and N-GSDME mRNA and protein in the pyroptosis pathway (P<0.05). Compared with the control group, the level of ROS in the baicalin group was significantly increased (P<0.05), and the content of ROS in the NAC group was significantly decreased (P<0.05). Compared with the NAC group, the content of ROS in the NAC + baicalin group was increased. Baicalin significantly attenuated the inhibitory effect of NAC on ROS production (P<0.05). Similarly, Western blot results showed that compared with the control group, the expression levels of pyroptosis-related proteins was increased in the baicalin group (P<0.05). NAC inhibited the expression of NLRP3 and reduced the cleavage of N-GSDMD and N-GSDME (P<0.05). Compared with the NAC group, the NAC + baicalin group had significantly increased expression of pyroptosis-related proteins. These results indicate that baicalin can effectively induce pyroptosis in DB cells and reverse the inhibitory effect of NAC on ROS production. CONCLUSION Baicalin can inhibit the proliferation of DLBCL cell line DB, and its mechanism may be through regulating ROS production to affect the pyroptosis pathway.
Humans ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism* ; Reactive Oxygen Species/pharmacology* ; Pyroptosis ; Cell Line ; RNA, Messenger ; Lymphoma, Large B-Cell, Diffuse

[ Abstract] Objective To investigate the relationship between single nucleotide polymorphism (SNP) Fok (rs2228570 / rs10735810) of vitamin D receptor (VDR) gene and hypertensive disorder complicating pregnancy (HDCP) in Han nationality women of Qinghai province. Methods A total of 137 Han nationality HDCP subjects (HDCP group) and 146 Han nationality normal pregnant subjects (control group) were selected from Qinghai province. The Fok polymorphism typing in HCDP group and control group was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) . The mutation was confirmed by sequencing. SPSS 19. 0 statistical software was used to test whether there were significant differences between two groups in general clinical data, genotype and allele frequency distribution. Results The frequency of FF Ff ff genotype of Fok in HDCP group and control group were 51. 82%, 37. 96%, 10. 22% and 34. 93%, 43. 15%, 21. 92% respectively (

Objective To investigate the association between the interleukin 10 (IL-10) gene promoter region-592A/C (rs1800872) polymorphism and hypertensive disorders of pregnancy (HDP) in Han women of Qinghai province and to determine the expression of this gene in two groups (HDP group and healthy control group) preliminarily. Methods A total of 140 HDP patients (HDP group) and 140 normal pregnant women (control group) in Qinghai Province were selected. Using blood DNA as template, the IL-10-592A/C polymorphism typing of HDP group and control group was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and verified by sequencing. The expression of IL-10 mRNA in the placental tissues of the two groups was detected by Real-time PCR. Plasma IL-10 levels of the two groups were detected by ELISA. Results The frequencies of AA, AC and CC genotypes of IL-10 gene in HDP group and control group were 24. 29%, 44. 29%, 31. 42% and 13. 57%, 41. 43%, 45. 00% respectively, the difference in genotype distribution between the two groups was statistically significant (P<0. 05);AA genotype frequency in HDP group(24. 29%)was higher than that of control group(13. 57%)(P<0. 05), CC genotype frequency in HDP group (31. 42%) was lower than that in control group (45. 00%) (P < 0. 05), while there was no significant difference in genotype frequency of AC between the two groups (P<0. 05); The distribution of A and C allele frequencies of IL-10592A/C polymorphism was different between the two groups, and the A allele frequency of HDP group was higher than that of control group (

OBJECTIVE: To investigate the effect of baicalin on the growth of extranodal NK/T cell lymphoma (ENKTCL) cells and its related mechanism. METHODS: Normal NK cells and human ENKTCL cells lines SNK-6 and YTS were cultured, then SNK-6 and YTS cells were treated with 5, 10, 20 μmol/L baicalin and set control. Cell proliferation and apoptosis was detected by Edu method and FCM method, respectively, and expressions of BCL-2, Bax, FOXO3 and CCL22 proteins were detected by Western blot. Interference plasmids were designed and synthesized. FOXO3 siRNA interference plasmids and CCL22 pcDNA overexpression plasmids were transfected with PEI transfection reagent. Furthermore, animal models were established for validation. RESULTS: In control group and 5, 10, 20 μmol/L baicalin group, the proliferation rate of SNK-6 cells was (56.17±2.96)%, (51.92±4.63)%, (36.42±1.58)%, and (14.60±2.81)%, respectively, while that of YTS cells was (58.85±2.98)%, (51.38±1.32)%, (34.75±1.09)%, and (15.45±1.10)%, respectively. In control group and 5, 10, 20 μmol/L baicalin group, the apoptosis rate of SNK-6 cells was (5.93±0.74)%, (11.78±0.34)%, (28.46±0.44)%, and (32.40±0.37)%, respectively, while that of YTS cells was (7.93±0.69)%, (16.29±1.35)%, (33.91±1.56)%, and (36.27±1.06)%, respectively. Compared with control group, the expression of BCL-2 protein both in SNK-6 and YTS cells decreased significantly (P<0.001), and the expression of Bax protein increased in SNK-6 cells only when the concentration of baicalin was 20 μmol/L (P<0.001), while that in YTS cells increased in all three concentrations(5, 10, 20 μmol/L) of baicalin (P<0.001). The expression of FOXO3 protein decreased while CCL22 protein increased in ENKTCL cell lines compared with human NK cells (P<0.001), but the expression of FOXO3 protein increased (P<0.01) and CCL22 protein decreased after baicalin treatment (P<0.001). Animal experiments showed that baicalin treatment could inhibit tumor growth. The expression of CCL22 protein in ENKTCL tissue of nude mice treated with baicalin decreased compared with control group (P<0.01), while the FOXO3 protein increased (P<0.05). In addition, FOXO3 silencing resulted in the decrease of FOXO3 protein expression and increase of CCL22 protein expression (P<0.01, P<0.001). CONCLUSION Baicalin can inhibit proliferation and promote apoptosis of ENKTCL cell lines SNK-6 and YTS, up-regulate the expression of Bax protein, down-regulate the expression of BCL-2 protein, and down-regulate the expression of CCL22 protein mediated by FOXO3. Animal experiment shown that the baicalin can inhibit tumor growth. Baicalin can inhibit the growth and induce apoptosis of ENKTCL cells through FOXO3/CCL22 signaling pathway.
Animals ; Mice ; Humans ; Lymphoma, Extranodal NK-T-Cell/pathology* ; Forkhead Box Protein O3/metabolism* ; bcl-2-Associated X Protein/pharmacology* ; Mice, Nude ; Signal Transduction ; Apoptosis ; Proto-Oncogene Proteins c-bcl-2/metabolism* ; Chemokine CCL22/pharmacology*

Objective: To investigate the clinical effects of in situ perforation of preserved split scar matrix in combination with scalp transplantation and vacuum sealing drainage in the treatment of hypertrophic scar in non-functional sites after burns. Methods: A retrospective observational study was used. From June 2017 to June 2019, 33 patients (24 males and 9 females, aged 8-50 years) who met the inclusion criteria with hypertrophic scars in non-functional sites outside the face after burns were treated in General Hospital of TISCO (the Sixth Hospital of Shanxi Medical University). All patients underwent scalp transplantation after perforation of retained split scar matrix in situ (with scar thinning area of 90-500 cm²), and then the vacuum sealing drainage was performed. The hematoma and infection of wounds were observed on the 7^th day after operation. At the same time, the survival rate of skin grafting was observed and calculated. The flatness and thickness of the scar in the operative area were observed in 12 months after operation, and the itching and pain of the patients were recorded. Vancouver Scar Scale was used to score the scar of patients before operation and at 3, 6 and 12 months after operation. The healing time and hair growth of donor site were observed. Data were statistically analyzed with repeated analysis of variance, paired sample t test and bonferroni correction. Results: On the 7^th day after operation, local subcutaneous hematoma appeared in the wound of 2 patients, which healed after dressing change; no infection occurred. On the 7^th day after operation, the survival rate of skin grafting of patients was 94.6%-99.0%(96.8±1.2)%. Scar flatness was well, the thickness of scar was not significantly higher than that of normal skin in 12 months after operation, and the symptoms of itching pain of patients disappeared or significantly relieved. Vancouver Scar Scale scores of patients before operation and at 3, 6, and 12 months after operation were 12.1±2.8, 8.5±1.5, 7.6±1.6, 6.7±1.3, respectively, and the scores of 3, 6, and 12 months after operation were all significantly lower than that before operation (with t values of 4.48, 4.06, and 3.97, respectively, P<0.01). All the donor sites of the head healed well in 4-7 days after operation. By 3-6 months after operation, all patients had good hair growth in the donor site and achieved no scar healing. Conclusions: The treatment of hypertrophic scar in non-functional sites outside the face after burns by in situ perforation of preserved split scar matrix in combination with scalp transplantation and vacuum sealing drainage can effectively improve the appearance of hypertrophic scar in non-functional areas after burn and reduce its degree of hyperplasia, with scar-free donor site healing.
Adolescent ; Adult ; Burns/surgery* ; Child ; Cicatrix, Hypertrophic/surgery* ; Female ; Humans ; Male ; Middle Aged ; Negative-Pressure Wound Therapy ; Scalp/surgery* ; Skin Transplantation ; Young Adult

Objective To investigate the correlation between the polymorphism of 5,10-methylenetetrahydrofolate reductase ( MTHFR) gene rs!801131 and hypertensive disorders complicating pregnancy ( HDCP ) in Qinghai Han nationality. Methods The polymorphism of MTHFR rsl801131 in 120 pregnant women with HDCP (HDCP group) and 120 normal pregnant women ( control group) were detected by restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) and verified by sequencing. Results The frequencies of AA, AC, and CC genotype of MTHFR gene in the HDCP group were 56. 67% , 32. 50% , and 10. 83% respectively, and those in the control group were 74.17%, 23.33% and 2. 50% respectively (P<0. 05, the distribution of genotype was different significantly between the two groups). The frequency of AA genotype of HDCP group (56. 67%) was lower than that of control group (74. 17%, P<0. 05) , the frequency of CC genotype of HDCP group ( 10. 83%) was higher than that of control group ( 2. 50% , P< 0. 05) , while there was no significant difference in the frequency of AC genotype between HDCP group and control group ( P<0. 05). The frequency distribution of alleles A and C of MTHFR rsl801131 polymorphism was significantly different between the HDCP group and the control group (P<0. 001) , and the frequency of allele C in the HDCP group was significantly higher than that in the control group (X2 = 12. 229, 0R=L 574, 95% C/= 1. 181-2. 099, P<0. 001). Conclusion The polymorphism of MTHFR rsl801131 is related to the occurrence of HDCP in Qinghai Han population. The C gene might be the susceptibility gene of HDCP, and CC genotype might be the susceptibility genotype of HDCP.

Objective:To explore the perioperative factors which influence the occurrence of prolonged postoperative ileus (PPOI) in patients with Crohn′s disease (CD) .Methods:A retrospective case-control study was conducted. Clinical data of CD patients who underwent partial intestinal resection at Jinling Hospital of Medical School of Nanjing University from June 2019 to January 2021 were analyzed retrospectively. The patients were divided into PPOI group and non-PPOI group according to whether the PPOI happened or not. The differences in perioperative indexes between PPOI and non-PPOI groups were assessed by univariate analysis. Spearman rank correlation test was used to exclude variables with strong correlations. The receiver operating characteristic (ROC) curve was performed to determine the cut-off values of the variables with P<0.05 in univariate analysis and clinical significance, and the variables were converted into binary qualitative data. And then the data were analyzed by multivariate Logistic regression to determine the independent risk factors of PPOI. Results:A total of 314 patients were included, including 202 males and 112 females with the age of (39 ± 14) years old. Eighty-nine (28.3%) patients with PPOI were set as PPOI group, and 225 (71.7%) patients without PPOI were set as non-PPOI group. Compared with non-PPOI group, patients in PPOI group had longer surgery duration [128 (99, 166) min vs. 110 (85, 141) min, P = 0.006], more total opioids [2.9 (2.4, 3.3) MME/kg vs. 2.7 (2.4, 3.1) MME/kg, P = 0.011, MME: morphine milligram equivalent], more intraoperative infusion volume ( P = 0.009), more crystal solution volume ( P = 0.007), higher intraoperative in-out balance per body weight (IOB/BW) [18.8 (14.1, 25.5) ml/kg vs. 16.4 (12.5, 20.7) ml/kg, P = 0.005], lower minimum level of albumin within 3 days after surgery ( P = 0.019), higher maximum change of albumin level after surgery ( P = 0.024), lower minimum concentration of serum potassium within 3 days after surgery ( P = 0.007), and more infusion supplement on the first day after surgery ( P<0.001), and longer postoperative hospital stay ( P<0.001). In the correlation analysis, there were strong correlations between intraoperative infusion volume and IOB/BW ( r = 0.689, P<0.001), and intraoperative infusion volume and crystal fluid volume ( r = 0.986, P<0.001) respectively. Therefore, intraoperative infusion volume and crystal fluid volume were excluded from the multivariate analysis. The cut-off value of the surgery duration was 123.5 min by ROC curve, the total opioids was 2.84 MME/kg, IOB/BW was 21.08 ml/kg, the minimum level of albumin and the minimum concentration of serum potassium within 3 days after surgery were 29.15 g/L and 3.75 mmol/L respectively, the maximum change of albumin level after surgery was 7.25 g/L, and the fluid supplement on the first day after surgery was 2751.5 ml. In multivariate Logistic regression analysis, surgery duration ≥ 123.5 min ( OR = 2.162, 95% CI: 1.260-3.710, P = 0.005), IOB/BW≥21.08 ml/kg ( OR = 3.184, 95% CI: 1.817-5.581, P<0.001), maximum change of albumin level ≥7.25 g/L after surgery ( OR = 1.948, 95% CI: 1.110-3.419, P = 0.02) and fluid supplement ≥2751.5 ml on the first day after surgery ( OR = 2.845, 95% CI: 1.630-4.966, P<0.001) were the independent risk factors for PPOI. Conclusions:CD patients who have the factors of long surgery duration, high IOB/BW, big change of albumin level after surgery and much fluid supplement on the first day after surgery are prone to PPOI. Therefore, controlling IOB/BW and fluid supplement on the first day after surgery are important measures to reduce the incidence of PPOI.

Objective:To explore the perioperative factors which influence the occurrence of prolonged postoperative ileus (PPOI) in patients with Crohn′s disease (CD) .Methods:A retrospective case-control study was conducted. Clinical data of CD patients who underwent partial intestinal resection at Jinling Hospital of Medical School of Nanjing University from June 2019 to January 2021 were analyzed retrospectively. The patients were divided into PPOI group and non-PPOI group according to whether the PPOI happened or not. The differences in perioperative indexes between PPOI and non-PPOI groups were assessed by univariate analysis. Spearman rank correlation test was used to exclude variables with strong correlations. The receiver operating characteristic (ROC) curve was performed to determine the cut-off values of the variables with P<0.05 in univariate analysis and clinical significance, and the variables were converted into binary qualitative data. And then the data were analyzed by multivariate Logistic regression to determine the independent risk factors of PPOI. Results:A total of 314 patients were included, including 202 males and 112 females with the age of (39 ± 14) years old. Eighty-nine (28.3%) patients with PPOI were set as PPOI group, and 225 (71.7%) patients without PPOI were set as non-PPOI group. Compared with non-PPOI group, patients in PPOI group had longer surgery duration [128 (99, 166) min vs. 110 (85, 141) min, P = 0.006], more total opioids [2.9 (2.4, 3.3) MME/kg vs. 2.7 (2.4, 3.1) MME/kg, P = 0.011, MME: morphine milligram equivalent], more intraoperative infusion volume ( P = 0.009), more crystal solution volume ( P = 0.007), higher intraoperative in-out balance per body weight (IOB/BW) [18.8 (14.1, 25.5) ml/kg vs. 16.4 (12.5, 20.7) ml/kg, P = 0.005], lower minimum level of albumin within 3 days after surgery ( P = 0.019), higher maximum change of albumin level after surgery ( P = 0.024), lower minimum concentration of serum potassium within 3 days after surgery ( P = 0.007), and more infusion supplement on the first day after surgery ( P<0.001), and longer postoperative hospital stay ( P<0.001). In the correlation analysis, there were strong correlations between intraoperative infusion volume and IOB/BW ( r = 0.689, P<0.001), and intraoperative infusion volume and crystal fluid volume ( r = 0.986, P<0.001) respectively. Therefore, intraoperative infusion volume and crystal fluid volume were excluded from the multivariate analysis. The cut-off value of the surgery duration was 123.5 min by ROC curve, the total opioids was 2.84 MME/kg, IOB/BW was 21.08 ml/kg, the minimum level of albumin and the minimum concentration of serum potassium within 3 days after surgery were 29.15 g/L and 3.75 mmol/L respectively, the maximum change of albumin level after surgery was 7.25 g/L, and the fluid supplement on the first day after surgery was 2751.5 ml. In multivariate Logistic regression analysis, surgery duration ≥ 123.5 min ( OR = 2.162, 95% CI: 1.260-3.710, P = 0.005), IOB/BW≥21.08 ml/kg ( OR = 3.184, 95% CI: 1.817-5.581, P<0.001), maximum change of albumin level ≥7.25 g/L after surgery ( OR = 1.948, 95% CI: 1.110-3.419, P = 0.02) and fluid supplement ≥2751.5 ml on the first day after surgery ( OR = 2.845, 95% CI: 1.630-4.966, P<0.001) were the independent risk factors for PPOI. Conclusions:CD patients who have the factors of long surgery duration, high IOB/BW, big change of albumin level after surgery and much fluid supplement on the first day after surgery are prone to PPOI. Therefore, controlling IOB/BW and fluid supplement on the first day after surgery are important measures to reduce the incidence of PPOI.