Objective To observe the effects of compound rhodiola sachalinensis A Bor (cRSAB) pretreatment on the serum levels of Interleukin -6 ( IL-6) and matrix metalloproteinase-9 ( MMP-9) at high altitude regions, so as to explore the mechanism of cRSAB for the elevation of hypoxia tolerance and the prevention of acute high altitude sickness .Methods Interventions were made on the 8 healthy subjects in accordance with different pressures (sea level pressure or simulated high altitude of 4000 m), exercise (rest state or stepping-stool exercise) and oral taking of cRSAB (7 days or 14 days).The subjects were divided into 7 groups: the conventional pressure at rest group (or group A), the conventional pressure exercise group (or group B), the hypobaric at rest group (or group C); the hypobaric exercise group (or group D), the oral-taking of cRSAB for 14 days at rest group (or group E), the oral-taking of cRSAB for 7 days exercise group (or group F), the oral-taking of cRSAB for 14 days exercise group (or group G).Then, serum concentrations of IL-6 and MMP-9 at different conditions were detected by ELISA .Results As compared with those of group A[IL-6(27.93 ±1.24)pg/ml, MMP-9(270.08 ±10.90)ng/ml], the serum concentrations of IL -6 and MMP-9 in the subjects of group C [(28.96 ±1.38) pg/ml], MMP-9 [(281.50 ±12.31) ng/ml] were elevated significantly.When compared with those of group B [ IL-6 (27.74 ±1.06) pg/ml, MMP-9 (269.88 ± 9.90)ng/ml], the serum concentrations of IL -6 and MMP-9 in the group D subjects [(29.56 ±1.26)pg/ml], MMP-9 [(285.25 ±11.67) ng/ml] increased obviously.Statistical significance could be noticed , when comparisons were made between them ( P <0.05).The serum concentrations of IL -6 and MMP-9 in the subjects of group C increased, as compared with those in the subjects of group D , with statistical significance (P <0.05).At the simulated high altitude of 4000 m, the serum concentrations of IL-6 and MMP-9 in the subjects of group F and group G all decreased considerably , when compared with those in subjects of group D , also with statistical significance (P <0.05).There was no statistical significance in serum concentrations of IL -6 and MMP-9, when comparisons were made between the subjects of group F and group G ( P >0.05). Conclusions At hypobaric pressure, the serum concentrations of IL-6 and MMP-9 in the subjects with exercise increased obviously.IL-6 and MMP-9 might be associated with the mechanism of cRSAB in the protective effect of low pressures on human motor function .

Objective To observe the effects of compound rhodiola sachalinensis A Bor (cRSAB) pretreatment on the serum levels of Interleukin -6 ( IL-6) and matrix metalloproteinase-9 ( MMP-9) at high altitude regions, so as to explore the mechanism of cRSAB for the elevation of hypoxia tolerance and the prevention of acute high altitude sickness .Methods Interventions were made on the 8 healthy subjects in accordance with different pressures (sea level pressure or simulated high altitude of 4000 m), exercise (rest state or stepping-stool exercise) and oral taking of cRSAB (7 days or 14 days).The subjects were divided into 7 groups: the conventional pressure at rest group (or group A), the conventional pressure exercise group (or group B), the hypobaric at rest group (or group C); the hypobaric exercise group (or group D), the oral-taking of cRSAB for 14 days at rest group (or group E), the oral-taking of cRSAB for 7 days exercise group (or group F), the oral-taking of cRSAB for 14 days exercise group (or group G).Then, serum concentrations of IL-6 and MMP-9 at different conditions were detected by ELISA .Results As compared with those of group A[IL-6(27.93 ±1.24)pg/ml, MMP-9(270.08 ±10.90)ng/ml], the serum concentrations of IL -6 and MMP-9 in the subjects of group C [(28.96 ±1.38) pg/ml], MMP-9 [(281.50 ±12.31) ng/ml] were elevated significantly.When compared with those of group B [ IL-6 (27.74 ±1.06) pg/ml, MMP-9 (269.88 ± 9.90)ng/ml], the serum concentrations of IL -6 and MMP-9 in the group D subjects [(29.56 ±1.26)pg/ml], MMP-9 [(285.25 ±11.67) ng/ml] increased obviously.Statistical significance could be noticed , when comparisons were made between them ( P <0.05).The serum concentrations of IL -6 and MMP-9 in the subjects of group C increased, as compared with those in the subjects of group D , with statistical significance (P <0.05).At the simulated high altitude of 4000 m, the serum concentrations of IL-6 and MMP-9 in the subjects of group F and group G all decreased considerably , when compared with those in subjects of group D , also with statistical significance (P <0.05).There was no statistical significance in serum concentrations of IL -6 and MMP-9, when comparisons were made between the subjects of group F and group G ( P >0.05). Conclusions At hypobaric pressure, the serum concentrations of IL-6 and MMP-9 in the subjects with exercise increased obviously.IL-6 and MMP-9 might be associated with the mechanism of cRSAB in the protective effect of low pressures on human motor function .