Objective To investigate the correlation of serum estradiol(E2),relaxin(RLX)levels and pelvic floor ultrasound parameters with the severity of postpartum stress urinary incontinence(PSUI)in patients.Methods A total of 144 patients with PSUI were enrolled in the PSUI group.According to the severity of the disease,they were divided into mild(n=59),moderate(n=55)and severe groups(n=30).Another 91 healthy postpartum women were selected as control group during the same period.General data of the PSUI group and the control group were compared.Serum E2 and RLX levels in patients with different severity levels were analyzed.Pelvic floor ultrasound parameters among groups were compared.Multivariate Logistic regression analysis was used to screen the influen-cing factors for the occurrence of PSUI.The correlations of serum E2,RLX levels and pelvic floor ul-trasound parameters with the severity of PSUI were analyzed.Results The serum RLX level in the PSUI group was significantly higher than that in the control group,while the E2 level was significantly lower(P＜0.05).The serum E2 level in the severe group was significantly lower than that in the moderate group and mild group,while the RLX level was significantly higher(P＜0.05).The proportions of multiple births,vaginal delivery,family history of urinary incontinence,mediolateral episiotomy and high serum RLX level as well as low serum E2 level in the PSUI group were significantly higher than those in the control group(P＜0.05).In the mild group,moderate group and severe group,the bladder-urethral posterior angle(PUA),urethral tilt angle(UTA),levator hiatus area as well as Valsalva maneuver-related urethral rotation angle(URA),bladder neck descent(BND)at rest and during maximum Valsalva maneuver increased sequentially(P＜0.05).In the mild group,moder-ate group,and severe group,the bladder position(BDP)and bladder neck position(BNP)during Valsalva maneuver decreased sequentially(P＜0.05).The funnel formation rate of the internal ure-thral orifice in the PSUI group and the control group were 22.22％(32/144)and 6.59％(6/91),respectively.Multivariate Logistic regression analysis showed that parity,vaginal delivery,family history of urinary incontinence,mediolateral episiotomy and serum E2 and RLX levels were influen-cing factors for the occurrence of PSUI(P＜0.05).Serum RLX levels and pelvic floor ultrasound parameters in PSUI patients were positively correlated with disease severity(r=0.573,0.590,P＜0.05),while serum E2 levels were negatively correlated with disease severity(r=-0.584,P＜0.001).Conclusion Serum E2 levels are negatively correlated with the severity of PSUI,while se-rum RLX levels and pelvic floor ultrasound parameters are positively correlated with the severity of PSUI.Parity,vaginal delivery,family history of urinary incontinence,mediolateral episiotomy and serum E2 and RLX levels are influencing factors for the occurrence of PSUI.

ObjectiveTo investigate the change and potential role of Mindin protein in the treatment of chronic hepatitis B （CHB） with PEG-IFNα-2b. MethodsA total of 29 CHB patients who received the treatment with PEG-IFNα-2b in The Second Affiliated Hospital of Xi’an Jiaotong University from January 2018 to December 2019 were enrolled， and according to their clinical outcome， they were divided into cured group with 17 patients and uncured group with 12 patients. Peripheral blood samples were collected from both groups at baseline， 12 weeks， and 24 weeks to measure blood routine indices， liver function parameters， hepatitis B markers， and Mindin protein. HBsAg， alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， and Mindin protein at different time points were compared between the two groups. The independent-samples t test was used for comparison of normally distributed continuous data between two groups， and the Mann-Whitney U test was used for comparison of non-normally distributed continuous data between two groups； a Spearman correlation analysis was used to investigate correlation； a multiple linear regression analysis was used to investigate the influence of HBsAg and ALT on the content of Mindin protein. ResultsThe analysis of baseline data showed that there were significant differences in the levels of HBsAg， HBeAb， albumin， and albumin/globulin ratio between the cured group and the uncured group （all P<0.05）. The cured group tended to have a gradual increase in the level of Mindin， and the level of Mindin at 24 weeks was significantly higher than that at baseline （P<0.05）. The cured group had a significantly higher level of Mindin protein than the uncured group at 24 weeks （P=0.019）. The cured group had a significantly lower level of HBsAg than the uncured group （P<0.05）， with a significant change from baseline to each time point within the cured group （P<0.05）. In addition， the levels of ALT and AST in the cured group tended to first increase and then decrease， and the expression levels at 12 weeks were significantly higher than those at baseline （P<0.05）. At 12 weeks， there was a strong linear correlation between Mindin protein levels and ALT in the untreated group （r=0.760 8， P<0.05）， and further multiple linear regression analysis also demonstrated a linear relationship between the two （b=1.571， P=0.019）. ConclusionThere is a significant difference in the level of Mindin protein between the cured group and the non-cured group after 24 weeks of PEG-IFNα-2b antiviral treatment， and therefore， detecting the dynamic changes of Mindin protein can better predict the treatment outcome of CHB， which provides a reference for clinical practice.

【Objective】 To study the expression levels of suppressor of cytokine signaling 1 (SOCS1) and its clinical significance in hepatitis B virus (HBV)-related liver diseases. 【Methods】 For this study we enrolled 25 patients with chronic hepatitis B (CHB), hepatitis B cirrhosis, or HBV-associated chronic acute liver failure (HBV-ACLF), and 25 healthy controls. The expression levels of SOCS1 mRNA in peripheral blood mononuclear cells (PBMCs) were determined using the RT-PCR method. The levels of SOCS1 and interleukin-6 (IL-6) in the plasma of patients with chronic liver diseases and healthy controls were measured using the ELISA method. The relative expression levels of SOCS1, SOCS1 mRNA, and other laboratory test indicators such as HBV-DNA, alanine aminotransferase (ALT), aspartate aminotransferase (AST), prothrombin activity (PTA) and total bilirubin (TBil) were compared among the groups. Additionally, the correlation between the expression levels of SOCS1 mRNA and the aforementioned laboratory indicators was assessed. 【Results】 The expression levels of SOCS1 mRNA and serum SOCS1 were highest in the HBV-ACLF group, followed by the cirrhosis group, and lowest in the healthy control group, with statistically significant differences (F=109.65, P<0.001). The relative expression of SOCS1 mRNA was positively correlated with TBil (r=0.89, P<0.001), ALT (r=0.89, P<0.001), AST (r=0.84, P<0.001) and IL-6 (r=0.93, P<0.001), but negatively correlated with PTA (r=-0.89, P<0.001) and was not significantly correlated with HBV-DNA (P=0.28). 【Conclusion】 The expression levels of SOCS1 in patients with HBV-related chronic liver diseases can reflect the severity of the disease and show a significant correlation with indicators used to assess the severity of liver diseases.