Objective:To establish fingerprints of Faeces Bombycis and simultaneously determine the content of four amino acids; To evaluate the quality of Faeces Bombycis from different regions. The fingerprint of Faeces Bombycis was established and the contents of 4 amino acids were determined.Methods:Kromasil 100-5 C18 (4.6 mm×250 mm, 5 μm) column was used for phenyl isothiocyanate (PITC) pre-column derivation-high performance liquid chromatography with acetonitrile-0.1 mol/L sodium acetate solution (pH adjusted to 6.5 with acetic acid) (7:93) mixed solution, and acetonitrile-water (4:1) mixed solution were mobile phase for gradient elution. The flow rate was 1.0 ml/min; the column temperature was 35 ℃; the detection wavelength was 254 nm; the injection amount was 5 μl. The fingerprints of 17 batches of Faeces Bombycis were established, the common peaks were identified by comparison of reference materials, and the similarity evaluation and principal component analysis (PCA) were carried out. The contents of glycine, alanine, proline and phenylalanine were determined simultaneously.Results:A total of 12 common peaks were identified from the fingerprints of Faeces Bombycis, and 12 amino acids were identified. The similarity of 17 batches of samples was greater than 0.95. PCA analysis showed that the regional difference of the quality of Faeces Bombycis was not significant. Faeces Bombycis produced in Qujing city in Yunnan Province had the highest total contents of 4 amino acids.Conclusion:The method has good repeatability and can provide reference for the quality evaluation and standard improvement of Faeces Bombycis.

Objective To discuss the application value of new non-invasive dynamic monopole radiofrequency (RF) system in body shaping.Methods 26 patients were included in this study,all female,aged 31 to 56 years.Application of new type of dynamic monopole radio frequency therapeutic apparatus (UK BTL Exilis therapeutic apparatus) was used to treat abdominal fat,proud flesh between the waist,back,hips,thighs and legs,upper arm area shape and the skin four times,with each time interval of 7～10 days.The patients filled in the subjective preliminary assessment questionnaire of satisfaction.Results All the patients had varying degrees of improvement in relaxation and partial fat reduction,with 76.9％ of satisfactory rate.No adverse reactions and no skin burns occured.Conclusions The new non-invasive dynamic monopole RF system can be used to improve the degree of relaxation and local fat reduction in the body molding,with high satisfaction.