Objective: As the coronavirus disease 2019 (COVID-19) pandemic has been prolonged, the pre-hospital screening test performed in hospitals has changed from polymerase chain reaction (PCR) to rapid antigen test (RAT). RAT has lower sensitivity than PCR, but a lower cost and shorter test time. In the hospital where this study was conducted, the preadmission COVID-19 test was changed from PCR to RAT on February 24, 2023. This study compared the efficacy of PCR and RAT as the pre-admission COVID-19 test. Methods: This was a retrospective observational study comparing two periods, 90 days from November 25, 2022 to February 23, 2023, and 90 days from February 28, 2023 to May 29, 2023. Patients hospitalized from the emergency medical center during these two periods were included. Binomial logistic regression was used to compare the emergency department length of stay (ED LOS) between the two groups (PCR vs. RAT). Logistic regression was used to compare the mortality. Results: Four thousand seven hundred and thirty-eight patients were admitted through the emergency room: 2,202 patients in the PCR group and 2,443 patients in the RAT group. No statistically significant differences in the characteristics and severity were observed between the two groups. The mean ED LOS of PCR 5.07 hours (interquartile range, 3.80-6.75) was longer than RAT 3.65 hours (interquartile range, 2.83-4.68) (P<0.001). Mortality was higher in PCR 8.9% than RAT 6.6% (P=0.005). Conclusion The study showed that ED LOS and mortality were reduced when using RAT rather than PCR as a preadmission COVID-19 test.

Background: The aim of this study is to investigate the specific pathway involved in human leukocyte antigen (HLA) sensitization using single-cell RNA-sequencing analysis and an allo-sensitized mouse model developed with an HLA.A2 transgenic mouse. Methods: For sensitization, wild-type C57BL/6 mouse received two skin grafts from C57BL/6-Tg(HLA-A2.1)1Enge/J mouse (allogeneic mouse, ALLO). For syngeneic control (SYN), skin grafts were transferred from C57BL/6 to C57BL/6. We performed single-cell RNA-sequencing analysis on splenocytes isolated from ALLO and SYN and compared the gene expression between them. Results: We generated 9,190 and 8,890 single-cell transcriptomes from ALLO and SYN, respectively. Five major cell types (B cells, T cells, natural killer cells, macrophages, and neutrophils) and their transcriptome data were annotated according to the representative differentially expressed genes of each cell cluster. The percentage of B cells was higher in ALLO than it was in SYN. Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that the highly expressed genes in the B cells from ALLO were mainly associated with antigen processing and presentation pathways, allograft rejection, and the Th17 cell differentiation pathway. Upregulated genes in the T cells of ALLO were involved in the interleukin (IL)-17 signaling pathway. The ratio of Th17 cluster and Treg cluster was increased in the ALLO. On flow cytometry, the percentage of Th17 (IL-17+/CD4+ T) cells was higher and regulatory T cells (FOXP3+/CD4+ T) was lower in the ALLO compared to those in the SYN. Conclusion Our results indicate that not only the B cell lineage but also the Th17 cells and their cytokine (IL-17) are involved in the sensitization to HLA.

Purpose: The purpose of this study was to compare the positive outcomes of fixed night shift, such as fatigue recovery experiences, work-life balance, and retention intentions, among nurses who had experienced both rotating shifts and fixed night shift. Methods: A single-cohort pre-post study design was used to compare the fatigue recovery experience, work-life balance, and retention intentions of nurses who have experienced fixed night shift. The study included 59 nurses working at a single university hospital in Seoul from September 1, 2023, to December 31, 2023. Data were analyzed using frequency and percentage, mean and standard deviation, t-test and ANOVA, paired t-test using the R statistical program. Results: The results revealed that acute and chronic fatigue levels decreased among nurses of night shifts during fatigue recovery assessment.However, the inter-shift recovery score did not improve, indicating that nurses' fatigue recovery was insufficient. Work-life balance scores increased, particularly in the area of work-leisure balance. Retention intention also improved among nurses of fixed night shift, but this was not statistically significant. Conclusion Based on the results of this study, the successful establishment of a fixed night shift system will reduce the turnover rate of the next generation of nurses and create a medical environment where nurses can spend more time on direct patient nursing care. Furthermore, we hope to improve the quality of life of nurses and improve the health outcomes of patients.

Purpose: The purpose of this study was to compare the positive outcomes of fixed night shift, such as fatigue recovery experiences, work-life balance, and retention intentions, among nurses who had experienced both rotating shifts and fixed night shift. Methods: A single-cohort pre-post study design was used to compare the fatigue recovery experience, work-life balance, and retention intentions of nurses who have experienced fixed night shift. The study included 59 nurses working at a single university hospital in Seoul from September 1, 2023, to December 31, 2023. Data were analyzed using frequency and percentage, mean and standard deviation, t-test and ANOVA, paired t-test using the R statistical program. Results: The results revealed that acute and chronic fatigue levels decreased among nurses of night shifts during fatigue recovery assessment.However, the inter-shift recovery score did not improve, indicating that nurses' fatigue recovery was insufficient. Work-life balance scores increased, particularly in the area of work-leisure balance. Retention intention also improved among nurses of fixed night shift, but this was not statistically significant. Conclusion Based on the results of this study, the successful establishment of a fixed night shift system will reduce the turnover rate of the next generation of nurses and create a medical environment where nurses can spend more time on direct patient nursing care. Furthermore, we hope to improve the quality of life of nurses and improve the health outcomes of patients.

Purpose: The purpose of this study was to compare the positive outcomes of fixed night shift, such as fatigue recovery experiences, work-life balance, and retention intentions, among nurses who had experienced both rotating shifts and fixed night shift. Methods: A single-cohort pre-post study design was used to compare the fatigue recovery experience, work-life balance, and retention intentions of nurses who have experienced fixed night shift. The study included 59 nurses working at a single university hospital in Seoul from September 1, 2023, to December 31, 2023. Data were analyzed using frequency and percentage, mean and standard deviation, t-test and ANOVA, paired t-test using the R statistical program. Results: The results revealed that acute and chronic fatigue levels decreased among nurses of night shifts during fatigue recovery assessment.However, the inter-shift recovery score did not improve, indicating that nurses' fatigue recovery was insufficient. Work-life balance scores increased, particularly in the area of work-leisure balance. Retention intention also improved among nurses of fixed night shift, but this was not statistically significant. Conclusion Based on the results of this study, the successful establishment of a fixed night shift system will reduce the turnover rate of the next generation of nurses and create a medical environment where nurses can spend more time on direct patient nursing care. Furthermore, we hope to improve the quality of life of nurses and improve the health outcomes of patients.