Objective:To investigate influence of Gegen Qinlian Decoction(GQD)on anti-inflammatory,anti-oxidative stress activities and related pathways in rats with non-alcoholic steatohepatitis(NASH),and to clarify its mechanism.Methods:NASH rat model was established with methionine choline deficiency feed,and blank group,model group,positive group and different doses of GQD administration groups were set.By analyzing rat serum inflammation indicators,liver tissue antioxidant capacity indicators,liver tissue pathological sections,and combined with Western blot and RT-qPCR technology,anti-inflammatory and anti-oxidative stress mechanism of GQD on NASH rats were finally clarified.Results:Each dose of GQD could significantly reduce serum levels of IL-6,IL-8,IL-10,IFN-α and IFN-β in NASH model rats.GQD medium and high doses groups were statistically significant compared with model group and positive group;each dose of GQD could significantly reduce contents of ALT,AST and MDA in liver tissue of NASH rats,and significantly increase SOD,GPX,T-AOC contents at the same time;Western blot and RT-qPCR results showed that GQD could significantly reduce expressions of PI3K,AKT,p-FOXO1 protein and mRNA in liver tissue of NASH model,significantly increase expressions of FOXO1,SIRT1,Catalase protein and mRNA,and compared with positive group at high doses,it was also statistically significant.Conclusion:GQD can treat NASH by regulating PI3K/AKT/FOXO1 signaling pathway and exerting anti-inflammatory and anti-oxidative stress effects.

This study explored whether Astragalus polysaccharide(APS)can regulate the VEGF/VEGFR signaling pathway to affect the proliferative activity of rat intestinal mucosal microvascu-lar endothelial cells(RIMMVECs).RIMMVECs were isolated from newborn rats,then purified and treated with APS at concentrations of 0.1,1.0,10.0,100.0,1 000.0,and 10 000.0 mg/L.MTT was used to determine the effect of APS on RIMMVECs proliferation and screen for the optimal concentration of APS.Subsequently,flow cytometry was used to detect the changes in cell cycle to evaluate the stage of action of APS on the cell cycle in RIMMVECs.Then,the ELISA was used to detect the changes of VEGFA in cell supernatant to evaluate the potential of cell proliferation and angiogenesis.The changes in fluorescence intensity of Fluo-8AM was observed using fluorescence microscopy to evaluate intracellular Ca2+levels.Finally,Western blot was used to detect the ex-pression of PERK in RIMMVECs to analyze the possible mechanism of APS.The results showed that 100 mg/L APS significantly enhanced the proliferative activity of RIMMVECs,increased the content of VEGFA in the cell supernatant,the intracellular Ca2+levels,and the expression of PERK protein,indicating that APS promotes the proliferation of RIMMVECs,which may be a-chieved by promoting the expression of VEGFA and activating the ERK pathway.

Objective:To investigate influence of Gegen Qinlian Decoction(GQD)on anti-inflammatory,anti-oxidative stress activities and related pathways in rats with non-alcoholic steatohepatitis(NASH),and to clarify its mechanism.Methods:NASH rat model was established with methionine choline deficiency feed,and blank group,model group,positive group and different doses of GQD administration groups were set.By analyzing rat serum inflammation indicators,liver tissue antioxidant capacity indicators,liver tissue pathological sections,and combined with Western blot and RT-qPCR technology,anti-inflammatory and anti-oxidative stress mechanism of GQD on NASH rats were finally clarified.Results:Each dose of GQD could significantly reduce serum levels of IL-6,IL-8,IL-10,IFN-α and IFN-β in NASH model rats.GQD medium and high doses groups were statistically significant compared with model group and positive group;each dose of GQD could significantly reduce contents of ALT,AST and MDA in liver tissue of NASH rats,and significantly increase SOD,GPX,T-AOC contents at the same time;Western blot and RT-qPCR results showed that GQD could significantly reduce expressions of PI3K,AKT,p-FOXO1 protein and mRNA in liver tissue of NASH model,significantly increase expressions of FOXO1,SIRT1,Catalase protein and mRNA,and compared with positive group at high doses,it was also statistically significant.Conclusion:GQD can treat NASH by regulating PI3K/AKT/FOXO1 signaling pathway and exerting anti-inflammatory and anti-oxidative stress effects.

This study explored whether Astragalus polysaccharide(APS)can regulate the VEGF/VEGFR signaling pathway to affect the proliferative activity of rat intestinal mucosal microvascu-lar endothelial cells(RIMMVECs).RIMMVECs were isolated from newborn rats,then purified and treated with APS at concentrations of 0.1,1.0,10.0,100.0,1 000.0,and 10 000.0 mg/L.MTT was used to determine the effect of APS on RIMMVECs proliferation and screen for the optimal concentration of APS.Subsequently,flow cytometry was used to detect the changes in cell cycle to evaluate the stage of action of APS on the cell cycle in RIMMVECs.Then,the ELISA was used to detect the changes of VEGFA in cell supernatant to evaluate the potential of cell proliferation and angiogenesis.The changes in fluorescence intensity of Fluo-8AM was observed using fluorescence microscopy to evaluate intracellular Ca2+levels.Finally,Western blot was used to detect the ex-pression of PERK in RIMMVECs to analyze the possible mechanism of APS.The results showed that 100 mg/L APS significantly enhanced the proliferative activity of RIMMVECs,increased the content of VEGFA in the cell supernatant,the intracellular Ca2+levels,and the expression of PERK protein,indicating that APS promotes the proliferation of RIMMVECs,which may be a-chieved by promoting the expression of VEGFA and activating the ERK pathway.

This study was designed to investigate the effect of graphene far-infrared irradiation on the relevant immune indexes of Lewis lung cancer tumor bearing mice after cisplatin chemotherapy.A lung cancer tumor-bearing mouse immune damage model was established in Lewis mice,and the model mice were divided into a model control group(CTL),a chemotherapy group(DDP),a low-power graphene group(G-Low,30℃),and a high-power graphene group(G-High,35℃).Mice in all groups were subjected to detect the tumor volume growth rate,thymus index and spleen index,spleen lymphocyte proliferation activity and NK cell killing activity,the number of white blood cells in the peripheral blood and the ratio of CD4+T/CD8+T in the peripheral blood.Compared with the CTL group,the thymus index and spleen index of the DDP group were significantly lower(P＜0.01);compared with the DDP group,the tumor inhibition rate of the G-Low group was significantly improved(P＜0.05),and the tumor inhibition rate of the G-High group was extremely significantly improved(P＜0.01).The thymus index,white blood cell count,peripheral blood CD4+T/CD8+T ratio,and NK cell killing activity of the spleen in the G-High group were significantly higher than those in the DDP group(P＜0.05).Taken together,Graphene far-infrared irradiation has a good repair effect on the immune damage caused by cisplatin chemotherapy in Lewis lung cancer tumor-bearing mice,and can significantly inhibit tumor growth.

This study was designed to investigate the effect of graphene far-infrared irradiation on the relevant immune indexes of Lewis lung cancer tumor bearing mice after cisplatin chemotherapy.A lung cancer tumor-bearing mouse immune damage model was established in Lewis mice,and the model mice were divided into a model control group(CTL),a chemotherapy group(DDP),a low-power graphene group(G-Low,30℃),and a high-power graphene group(G-High,35℃).Mice in all groups were subjected to detect the tumor volume growth rate,thymus index and spleen index,spleen lymphocyte proliferation activity and NK cell killing activity,the number of white blood cells in the peripheral blood and the ratio of CD4+T/CD8+T in the peripheral blood.Compared with the CTL group,the thymus index and spleen index of the DDP group were significantly lower(P＜0.01);compared with the DDP group,the tumor inhibition rate of the G-Low group was significantly improved(P＜0.05),and the tumor inhibition rate of the G-High group was extremely significantly improved(P＜0.01).The thymus index,white blood cell count,peripheral blood CD4+T/CD8+T ratio,and NK cell killing activity of the spleen in the G-High group were significantly higher than those in the DDP group(P＜0.05).Taken together,Graphene far-infrared irradiation has a good repair effect on the immune damage caused by cisplatin chemotherapy in Lewis lung cancer tumor-bearing mice,and can significantly inhibit tumor growth.