Objective To investigate the expression and clinical significance of miR-483-3p in patients with gesta-tional diabetes mellitus(GDM).Methods A total of 100 GDM patients with 24-32 weeks of gestation who under-went routine obstetric examination in Anqiu People's Hospital were selected as the normal glucose tolerance(NGT)group,and a total of 98 healthy pregnant women of the same age of 24-32 gestational weeks were selected as the NGT group.The expression level of miR-483-3p was detected by RT-qPCR,the diagnostic value of miR-483-3p in GDM was analyzed by receiver operating characteristic(ROC)curve,the correlation between the expression levels of miR-483-3p and clinical indicators in the GDM group was analyzed by chi-square test,and the risk factors of GDM were analyzed by Logistic regression.Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-483-3p and autophagy-related protein 7(ATG7).CCK8,flow cytometry and Transwell assays were used to detect the proliferation,apoptosis,and migration and invasion ability of cells,respectively.Results The expression level of miR-483-3p in the serum of patients in the GDM group was significantly higher than that in the NGT group.The expression of miR-483-3p had diagnostic value for GDM.Pre-pregnancy BMI,FBG,FINS,HOMA-IR and TG were significantly correlated with serum miR-483-3p expression.In addition,BMI and TG before pregnancy were risk factors leading to GDM.miR-483-3p regulated HG-treated HTR-8/SVneo cell proliferation,apoptosis,migration and invasion by targeting at ATG7.Conclusions miR-483-3p is involved in the disease pro-gression of GDM and is a potential biomarker of GDM diagnosis.

To identify factors associated with the recurrence of polymyalgia rheumatica(PMR) within one year.

Objective To investigate the expression of Smac/DIABLO, XIAP mRNA in acute pancreatitis (AP) and the relationship with the severity in rats.Methods Fifty-four SD rats were randomly divided into three groups:sham-operation (SO) group, acute edematous pancreatitis (AEP) group and acute necrotizing pancreatitis (ANP) group.The models of AEP and ANP were induced by retrograde injection of 1％ and 3.5％ sodium deoxycholate into the pancreaticobiliary duct respectively.The specimens of pancreatic tissue at 3 h, 6 h, 12 h were collected, pathological changes of the pancreas were observed, apeptosis in pancreas were detected by TUNEL method and the expression of Smac/DIABLO, XIAP mRNA were analyzed by real-time PCR.Results Pathological changes of the pancreas confirmed the establishment of AEP and ANP.Apeptosis indexes in SO group, AEP group and ANP group were 0.67±0.82, 6.62 ±0.78 and 4.70 ±0.82, and the differences were significant (P< 0.05).The expression of Smac/DIABLO mRNA of AEP group increased with time, while the expression of ANP group decreased with time.Compared with SO group, Smac/DIABLO mRNA expressions at 6 h in AEP and ANP group were 2.41 ± 0.92 and 1.47± 0.53, and the differences were significant (P<0.05).By contrast, the expressions of XIAP mRNA in AEP group decreased with time,while the expressions in ANP group increased with time.The expressionsof XIAP mRNA at 6 h in AEP and ANP group were 5.51 ± 1.07 and 6.99 ± 1.00, and the differences were significant (P<0.05).Conclusions In acute pancreatitis, the expression of Smac/DIABLO mRNA was consistent with the apoptosis of pancreatic acinar cells, but not consistent with the severity of pancreatitis.The expression of XIAP mRNA was consistent with the severity of pancreatitis.Smac/DIABLO, XIAP mRNA is associated with regulation of apoptosis.

Objective:To compare the differences of bond strength usi ng gold intermediate layer between Ni-Cr alloy and opaque porcelain(OP) baked at different temperatures. Methods:36 standard samples of Ni-Cr al loy and 36 of gold were prepared. The samples were divided into 4 groups with 9 in each. In group 1,OP was smeared to the surface of the samples and then baked at 950 ℃. In group 2,3 and 4 blendgold neu(a gold past) was put on the surface of the samples, then baked at 820 ℃,890 ℃ and 960 ℃ respectively. The samples were proccessed for further tests.The bond strength was tested by shear bond te st, and the electron probe was used to observe interfacial bond state and diffus ion of interfacial elements. Results:The bond strength(MPa) in g roup 1,2,3 and 4 was 19.66? 1.83,22.34?2.73,21.33?2.75 and 28.02?5.71 res pectively(group 1 vs group 4 P