The therapeutic efficacy and possible mechanism of action of superficial needling therapy for knee osteoarthritis(KOA)are reviewed by analyzing relevant medical literature.Superficial needling therapy exerts certain efficacy in treating KOA,and the clinical efficacy could be further improved by combining it with conventional acupuncture,as well as Chinese medicine and other acupuncture methods alone.The therapy of stimulation of meridian qi and invigoration of yang qiare in line with the pathogenesis of KOA.Superficial needling therapy exerts certain effect on KOA by enhancing acupuncture stimulation,enlarging the treatment area,and releasing muscle and fascia.

With the changes in lifestyle, marriages and childbearing concepts, and population policies in recent years, the proportion of women with advanced maternal age (AMA) in China is rising rapidly. AMA and maternal stress due to advanced age are considered as independent risk factors for maternal mental health and the neurobehavioral development of their offspring. In this paper, we summarized how the glial cell line-derived neurotrophic factor, the hypothalamic-pituitary-adrenal axis, the 5-HT system and the oxytocin affect the maternal mental health and the neurobehavioral development of their offspring, as well as some potential targeted interventions. Interventions currently being developed include artificial supplements, antidepressants, physical exercise, nutrient supplementation, etc., but they have a limited efficacy and a high risk of side effects, yet there is still a lack of specific and systematic interventions. The relevant pathogenesis should continue to be explored in the future research, and hopefully more effective and safe interventions will be given in the near future to improve the mental health of mothers with AMA and the long-term healthy development of their offspring.

With the changes in lifestyle, marriages and childbearing concepts, and population policies in recent years, the proportion of women with advanced maternal age (AMA) in China is rising rapidly. AMA and maternal stress due to advanced age are considered as independent risk factors for maternal mental health and the neurobehavioral development of their offspring. In this paper, we summarized how the glial cell line-derived neurotrophic factor, the hypothalamic-pituitary-adrenal axis, the 5-HT system and the oxytocin affect the maternal mental health and the neurobehavioral development of their offspring, as well as some potential targeted interventions. Interventions currently being developed include artificial supplements, antidepressants, physical exercise, nutrient supplementation, etc., but they have a limited efficacy and a high risk of side effects, yet there is still a lack of specific and systematic interventions. The relevant pathogenesis should continue to be explored in the future research, and hopefully more effective and safe interventions will be given in the near future to improve the mental health of mothers with AMA and the long-term healthy development of their offspring.

Objective:To explore the status and influencing factors of stigma in patients with pituitary adenoma.Methods:From January 2020 to May 2021, a total of 106 patients with pituitary adenoma in the Affiliated Huai'an No.1 People 's Hospital of Nanjing Medical University were selected using the convenience sampling method. Internalized Stigma of Mental Illness, Medical Coping Style Questionnaire and Connor-Davidson Resilience Scale were used to investigate them. Binary Logistic regression analysis was used to evaluate the influencing factors of stigma in patients with pituitary adenoma. Results:Among 106 patients with pituitary adenoma, 87 (82.08%) had stigma. The stigma score of patients with pituitary adenoma was (2.58±0.61) . Binary Logistic regression analysis results showed that family monthly income, coping style and mental elasticity were the influencing factors of stigma in patients with pituitary adenoma ( P<0.05) . Conclusions:Stigma is common in patients with pituitary adenoma. Medical staff should take targeted intervention measures according to the influencing factors of stigma in patients with pituitary adenoma, so as to help patients reduce stigma.

Depression is a major mood disorder. Abnormal expression of glial glutamate transporter-1 (GLT-1) is associated with depression. Schisantherin B (STB) is one bioactive of lignans isolated from Schisandra chinensis (Turcz.) Baill which has been commonly used as a traditional herbal medicine for thousands of years. This paper was designed to investigate the effects of STB on depressive mice induced by forced swimming test (FST). Additionally, we also assessed the impairment of FST on cognitive function in mice with different ages. FST and open field test (OFT) were used for assessing depressive symptoms, and Y-maze was used for evaluating cognition processes. Our study showed that STB acting as an antidepressant, which increased GLT-1 levels by promoting PI3K/AKT/mTOR pathway. Although the damage is reversible, short-term learning and memory impairment caused by FST test is more serious in the aged mice, and STB also exerts cognition improvement ability in the meanwhile. Our findings suggested that STB might be a promising therapeutic agent of depression by regulating the GLT-1 restoration as well as activating PI3K/AKT/mTOR pathway.
Animals ; Cognition ; Cognition Disorders ; Depression ; Glutamic Acid ; Herbal Medicine ; Learning ; Lignans ; Memory ; Mice ; Mood Disorders ; Physical Exertion ; Schisandra

Objective: To establish a preoperative evaluation model and evaluate the accuracy of postoperative complications by analyzing the risk factors of postoperative complications in hip arthroplasty in elderly patients, and to provide a basis for grading nursing. Methods: From January 2012 to August 2018, 148 patients with elderly patients undergoing hip arthroplasty admitted to People′s hospital of Wanning were included in the study. Among them, 23 patients with postoperative complications were included as complications and 125 patients without complications served as a control group. Univariate analysis of the factors associated with complications, statistically significant indicators were included in the multivariate logistic regression model, and the risk factors for postoperative complications in hip arthroplasty were analyzed. Based on the results of the regression analysis, a preoperative evaluation model was established. The ROC curve and the Hosmer-Lemeshow test were used to evaluate the model prediction performance. Results: Multivariate analysis showed age (OR=1.239, 95%CI: 1.002-1.531, P=0.048), Glu (OR=2.961, 95%CI: 1.055-8.311, P=0.039), surgical size (OR=4.997, 95%CI: 1.269-19.686, P=0.021) were risk factors for postoperative complications, Albumin (OR=0.696, 95%CI: 0.535-0.904, P=0.007) was a protective factor for postoperative complications (P<0.05). The ROC curve showed a nomogram model predicting the area under the complication curve (AUC) of 0.988 (95%CI:0.975-1.000), with a specificity of 96.77% and a sensitivity of 99.82%. Conclusion A nomogram model based on age, Glu, albumin, surgery size to predict the risk of complications after hip arthroplasty in elderly patients has a good specificity and sensitivity, high clinical value.

Objective To study the effect of atrial natriuretic peptide(ANP)deletion on melanoma growth. Methods A subcutaneous xenotransplanted melanoma model was established in ANP knockout mice and C57BL/6J mice. The tumor volume was measured at the sixth day after establishment of the subcutaneous transplantation. Tumor cell proliferation and tumor-induced angiogenesis were detected by immunohistochemical staining. Results The volume and weight of xenotransplanted melanoma were significantly decreased in the ANP knockout mice compared with those in the C57BL/6J mice. The proliferative tumor cells and microvessel density were significantly decreased in the tumor tissues of ANP knockout mice compared with those in the C57BL/6J mice. Conclusions ANP deletion significantly suppresses xenotransplanted melanoma growth through inhibiting the tumor cell proliferation and angiogenesis.

Objective To investigate the role of atrial natriuretic peptide (ANP) in lung metastasis of melanoma. Methods Melanoma B16F10 cells were intravenously injected into ANP knockout mice and C57BL／6J mice. After three weeks, the mice were sacrificed, and the lungs were removed, embedded, and examined by pathology using HE staining. The numbers of metastatic foci on the lung surface and micrometastatic foci in the lung tissues were counted. Results The ANP knockout mice displayed fewer metastatic foci on the lung surface and less micrometastatic foci in the lung tissues of ANP knockout mice than in the C57BL／6J mice. Conclusions ANP deletion significantly suppresses the metastasis of melanoma in the lung of mice.

To investigate the effects and mechanism of andrographolide(Andro)on type II diabetic nephropathy in Leprdb/dbmice,levels of urinary protein,serum creatinine and blood urea nitrogen after 24 h of Andro(1 mg/kg,ip)treatment on Leprdb/dbmice were measured by kit.The changes on renal pathology and sugar levels in diabetic mice treated by Andro were observed by HE staining and PAS staining.The expression of fibronectin (FN)and NOX-4 protein were analyzed by immunofluorescence.Results showed that levels of urinary protein, serum creatinine and blood urea nitrogen in Leprdb / dbmice after Andro treatment for 24 h were significantly lower than those of the control group. Kidney pathologic change was inhibited, and FN and NOX-4 expression were markedly decrease in the Andro group.In conclusion,Andro can significantly reduce the diabetic nephropathy in Leprdb/dbmice with spontaneous type II diabetes mellitus.

[Objective] To investigate the effect of P-selectin on the intestinal tumorigenesis in the 15,24-week-old ApcMin/+ mice.[Methods] Male ApcMin/+ mice were mated with female P-selectin knockout (P-sel-/-) mice to generate AMin/+Ps-/-mice.The diameters and numbers of the intestinal tumors in the intestines of ApcMin/+ and AM/+ps-/-mice were measured using an inverted microscope.The tumor volumes were measured using the following equation:volume =0.52 × (length × width2).[Results] There were no significant difference between the volume and number of intestinal tumor in 15-week-old ApcMin/+ mice and 15-week-old A+ P-mice.The volume and number of intestinal tumor were significantly increased in 24-week-old A+P-mice compared to 24-weekold ApcMin/+ mice.P-selectin deletion significantly prolonged the survival time of ApcMin/+ mice.[Conclusions] P-selectin deletion promotes the intestinal tumorigenesis in the 24-week-old ApcMin/+ mice.