Culture and purification, identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometer and biochemical assay, drug sensitive test, resistance gene detection and multilocus sequence typing were conducted for 4 strains of serotype 2 Streptococcus suis isolated from clinical specimens in 2021, Hainan Province. The results showed that Strain 1 and Strain 2 were ST7 type, Strain 3 was ST1 type, and Strain 4 was ST2302 type. The strains are sensitive to levofloxacin, penicillin, meropenem, linezolid, ceftriaxone, cefepime, and daptomycin. Three strains are resistant to tetracycline, erythromycin, and azithromycin, two strains are resistant to clindamycin and ampicillin, and one strain is resistant to chloramphenicol and vancomycin. There may be sources of Streptococcus suis infection in multiple regions of Hainan Province, and the ST molecular typing of Streptococcus suis is diverse.