Objective To explore the mechanism of malt alcohol extract improving depression-like behavior induced by CUMS in rats by regulating gut microbiota.Methods The depression model of rats was established using an 8-weeks CUMS procedure,and the administration group was given low(59.6 mg·kg-1)and high(178.8 mg·kg-1)doses of malt alcohol extract,respectively.The depression-like behavior of rats was evaluated by classic behavioral test.The composition of intestinal microbiota of rats was analyzed by 16S rRNA sequencing.The morphological changes of colon were observed by hematoxylin and eosin(HE),the expression of ZO-1 and Occludin in colon was detected by immunofluorescence(IF),and the expression of IL-10,IL-1βand 5-HT were detected by ELISA.Results The low dose of malt alcohol extract attenuated the depressive behavior and restored the expression of 5-HT in the brain of CUMS rats.16S rRNA sequencing results showed that the diversity and relative abundance of gut microbiota changed after treatment with the low dose of malt alcohol extract.ELISA results showed that the low dose of malt alcohol extract significantly reversed the CUMS-induced reduction of IL-10 and elevation of IL-1 β.HE results showed that the low dose of malt alcohol extract significantly ameliorated CUMS-induced structural damage in colon.IF results showed increased protain expression of intestinal epithelial barrier tight junction proteins ZO-1 and Occludin by the low dose of malt alcohol extract.Conclusion The low dose of malt alcohol extract can ameliorate CUMS-induced depressive-like behavior in rats by modulating intestinal flora,restoring 5-HT expression in the brain,inhibiting inflammation,and repairing the intestinal barrier.

Objective To explore the mechanism of malt alcohol extract improving depression-like behavior induced by CUMS in rats by regulating gut microbiota.Methods The depression model of rats was established using an 8-weeks CUMS procedure,and the administration group was given low(59.6 mg·kg-1)and high(178.8 mg·kg-1)doses of malt alcohol extract,respectively.The depression-like behavior of rats was evaluated by classic behavioral test.The composition of intestinal microbiota of rats was analyzed by 16S rRNA sequencing.The morphological changes of colon were observed by hematoxylin and eosin(HE),the expression of ZO-1 and Occludin in colon was detected by immunofluorescence(IF),and the expression of IL-10,IL-1βand 5-HT were detected by ELISA.Results The low dose of malt alcohol extract attenuated the depressive behavior and restored the expression of 5-HT in the brain of CUMS rats.16S rRNA sequencing results showed that the diversity and relative abundance of gut microbiota changed after treatment with the low dose of malt alcohol extract.ELISA results showed that the low dose of malt alcohol extract significantly reversed the CUMS-induced reduction of IL-10 and elevation of IL-1 β.HE results showed that the low dose of malt alcohol extract significantly ameliorated CUMS-induced structural damage in colon.IF results showed increased protain expression of intestinal epithelial barrier tight junction proteins ZO-1 and Occludin by the low dose of malt alcohol extract.Conclusion The low dose of malt alcohol extract can ameliorate CUMS-induced depressive-like behavior in rats by modulating intestinal flora,restoring 5-HT expression in the brain,inhibiting inflammation,and repairing the intestinal barrier.

Objective:To investigate the impacts of antenatal anxiety on preterm birth and low birth weight.Methods:Women in early pregnancy were recruited for follow-up, antenatal anxiety in three trimesters was screened using Self-Rating Anxiety Scale and the score ≥50 was regarded as anxiety. Logistic regression analysis was conducted to evaluate the associations of the anxiety in three trimesters, new onset anxiety in the second and third trimesters with infant birth outcomes, such as preterm birth and low birth weight.Results:The rates of anxiety in the first, second and third trimesters of pregnancy were 12.5%, 3.7%, and 7.4% respectively. We found that there was no statistical association between anxiety in the first and second trimester and preterm birth. The anxiety in the third trimester was associated with increased odds for preterm birth ( OR=3.55, 95 %CI: 1.62-7.82). Associations between anxiety in all three trimesters and low birth weight were not significant. New onset anxiety in the third trimester was associated with significant increased risk of premature delivery ( OR=5.20, 95 %CI: 1.84-14.70) and low birth weight ( OR=6.93, 95 %CI: 2.42-19.88). Conclusions:Our study showed that anxiety in the third trimester is an important risk factor for premature delivery, new onset anxiety symptoms in the third trimester can significantly increase the incidence of premature birth and low birth weight of infant.

Objective To investigate the effect of CICARE mode on communication skills training of new entry guidance personnel. Methods A total of 16 new attendants were set as the control group, and routine training was used (between May 2015 and June 2015). Another 18 new attendants named as the observation group were trained in CICARE mode on the basis of routine training (between May 2016 and June 2016). The communication skills and satisfaction degree were compared between two groups. Results The scores of establishing a harmonious relationship, keen to listen, to confirm the patient problem, participation, efficient information transfer, verifying the feeling after the new recruits training for guidance staff were 3.72±0.08, 3.77±0.17, 3.60±0.08, 2.96±0.32, 3.50±0.14, 3.06±0.50 in the observation group, 3.13 ± 0.11, 3.25 ± 0.24, 2.66 ± 0.12, 2.67 ± 0.37, 2.90 ± 0.08, 2.59 ± 0.19 in the control group, the differences were statistically significant (t =2.451-27.155, P<0.05). The scores of guiding service appearance, body language, active service, health consultation, medical order, answer questions and provide help, medical environment and overall satisfaction respectively was 99.67 ± 2.58, 98.00 ± 6.05, 98.00 ± 6.05, 96.00 ± 8.07, 99.33 ± 3.62, 98.00 ± 6.05, 96.00 ± 8.07, 94.00 ± 12.91, 97.50 ± 5.48 in the observation group, and 91.33 ± 10.56, 87.67 ± 14.19, 84.67 ± 14.31, 79.67 ± 15.27, 84.33 ± 14.17, 83.67 ± 14.37, 85.67 ± 12.70, 87.00 ± 10.85, 84.67 ± 9.91 in the control group, the differences were statistically significant (t=3.215-8.775, P<0.05). Conclusions CICARE mode can not only provide normative and orderly guidance for the guiding staff, but also improve the communication ability of the guiding staff in a short time, and let the patients participate in the guiding service. It is worthy of being popularize and applied in the guided diagnosis service to increase the patient′s participation, acceptance and satisfaction.

Aim To probe into the best conditions for the establishment of endometriosis model in rats by the orthogonal experiment method.Methods Autotransplantation was used to establish the endometriosis model in rats by the orthogonal experiment method in which four main factors were considered to optimize the condition of model,according to the volume of heterotopic endometrium.Results The para ligament of uterus was the best place for transplanting.Both dissecting muscular layer and injecting estrin played an important role in the establishment of endometriosis model in rats. The value of four factors decreased progressively according to the injection of estrin,the dissection of muscular layer,the direction of endometrium implantation and oestrus.Conclusions By optimizing the condition for the establishment of endometriosis model in rats by orthogonal experiment,the volume of heterotopic endometrium was improved.A technology platform for pharmacodynamics research was thus offered.