Objective To observe the clinical efficacy of uterine-sparing U-shaped reconstruction surgery in the treatment of diffuse uterine adenomyosis.Methods A total of 48 patients with diffuse uterine adenomyosis underwent surgical treatment from October 2019 to September 2022.All the patients underwent a modified lesion resection procedure via sagittal plane incision of the uterine corpus,enabling direct visualization for U-shaped excision of adenomyotic foci within the myometrium,followed by endometrial cavity reconstruction and uterine remodeling.The dysmenorrhea score,menstrual volume score,serum CA125,and uterine volume were assessed at 1,6,and 12 months postoperatively.Results All the 48 patients underwent surgery successfully.The average surgery time was(115.7±13.6)min,the average intraoperative blood loss was(109.4±30.9)ml,and the total length of hospital stay was(8.6±1.7)d.At 1,6,and 12 months after surgery,the dysmenorrhea score,menstrual volume score,serum CA125 levels,and uterine volume were significantly lower as compared to the pre-operative values(all P=0.000).The hemoglobin level at 1 month after surgery was significantly higher than that before surgery(P=0.000).No symptomatic or ultrasonic recurrences were observed in all the 48 cases.Two cases got unexpected pregnancy after surgery.Conclusions Uterine-sparing U-shaped reconstruction surgery is a safe and effective treatment for diffuse uterine adenomyosis.It does not require special instruments,and is simple and easy to perform.

Objective To observe the clinical efficacy of uterine-sparing U-shaped reconstruction surgery in the treatment of diffuse uterine adenomyosis.Methods A total of 48 patients with diffuse uterine adenomyosis underwent surgical treatment from October 2019 to September 2022.All the patients underwent a modified lesion resection procedure via sagittal plane incision of the uterine corpus,enabling direct visualization for U-shaped excision of adenomyotic foci within the myometrium,followed by endometrial cavity reconstruction and uterine remodeling.The dysmenorrhea score,menstrual volume score,serum CA125,and uterine volume were assessed at 1,6,and 12 months postoperatively.Results All the 48 patients underwent surgery successfully.The average surgery time was(115.7±13.6)min,the average intraoperative blood loss was(109.4±30.9)ml,and the total length of hospital stay was(8.6±1.7)d.At 1,6,and 12 months after surgery,the dysmenorrhea score,menstrual volume score,serum CA125 levels,and uterine volume were significantly lower as compared to the pre-operative values(all P=0.000).The hemoglobin level at 1 month after surgery was significantly higher than that before surgery(P=0.000).No symptomatic or ultrasonic recurrences were observed in all the 48 cases.Two cases got unexpected pregnancy after surgery.Conclusions Uterine-sparing U-shaped reconstruction surgery is a safe and effective treatment for diffuse uterine adenomyosis.It does not require special instruments,and is simple and easy to perform.

Objective:To screen and identify H-2 d-restricted T cell epitopes in fusion (F) and attachment (G) glycoproteins of Nipah virus (NiV) in mice. Methods:The complete peptides (single peptide contains 15 amino acids, and 10 amino acids were repeated in the front and back peptides) derived from F and G antigens were mixed into peptide libraries. BALB/c mice were immunized with DNA vaccines expressing NiV F and G proteins alone and in combination. The full sequence peptide libraries of F and G antigens were mixed into peptide pools by matrix design, and spleen cells of immunized mice were collected and analyzed by IFN-γ ELISPOT assay to detect the dominant H-2 d-restricted epitope peptides. Results:Twelve dominant H-2 d-restricted peptides were screened from the F protein-specific peptide library and the 56th peptide produced the strongest reaction. Four dominant peptides were screened from the G protein-specific peptide library and the 72nd peptide produced the strongest reaction. Conclusions:In this study, 12 F antigen-specific and 4 G antigen-specific H-2 d restricted dominant T cell epitopes of NiV were screened and identified by IFN-γ ELISPOT, which could provide reference for immunological analysis of NiV and vaccine research.