Objective To compare the therapeutic effect of acupuncture at Neiguan point(PC6),acupuncture on the upper limbs and traditional medication for motion sickness.Methods Totally 30 male KM mice were randomly divided into 5 groups(n=6):blank control group,model group(acceleration exposure),acupuncture PC6 group(acupuncture at PC6+acceleration exposure),acupuncture upper limbs group(acupuncture of the arm except PC6+acceleration exposure)and traditional medicine group(difenidol administered intragastrically+acceleration exposure).The motion sickness index(MSI)and the serum levels of serotonin(5-HT),adrenaline,glucagon,insulin and blood glucose were detected.Results MSI and serum 5-HT,adrenaline,glucagon,insulin and blood glucose levels in the model group were significantly higher than those in the blank control group(P<0.05).Compared with those in the model group,MSI and 5-HT,adrenaline,glucagon and blood glucose levels were decreased in the acupuncture PC6 group and traditional medicine group(P<0.05),while insulin level was not significantly changed(P>0.05).The adrenaline level in the traditional medicine group was lower than that in the acupuncture PC6 group(P<0.05).The acupuncture upper limbs group had lower MSI and higher insulin level than the model group(P<0.05),while there were no significant differences in the levels of 5-HT,adrenaline,glucagon or blood glucose between the two groups(P>0.05).Conclusion Both difenidol and acupuncture at PC6 can effectively relieve motion sickness.

OBJECTIVE To prepare hyperoside mixed nanomicelles （Hyp-F127/TPGS） and optimize its preparation technology，and to investigate its intestinal absorption characteristics. METHODS Hyp-F127/TPGS was prepared by thin film dispersion method. Based on single factor test and Plackett-Burman design ，combined with Box-Behnken response surface method ， the preparation process was optimized and validated using entrapped efficiency （EE）and drug loading （DL）as evaluation indexes ， F127-TPGS mass ratio ，hydration time and the amount of Hyp as factors. The appearance and microscopic morphology of Hyp-F127/TPGS obtained by the optimal technology were observed ，and the particle size ，polydispersity index （PDI）and Zeta potential were also determined. The critical micelle concentration （CMC）of blank micelle （F127/TPGS），in vitro release behavior and preliminary stability of Hyp-F 127/TPGS were investigated ，and absorption characteristics of Hyp-F 127/TPGS were investigated by in situ unidirectional intestinal perfusion model. RESULTS The optimal preparation technology of Hyp-F 127/TPGS included F127-TPGS mass ratio of 2∶1，hydration time of 2 h，and Hyp amount of 9 mg. Results of three validation tests showed that the EE of Hyp-F 127/TPGS was （87.20±0.99）％，and the DL was （5.02±1.20）％，deviations from predicted values were 0.92％ and 2.39％. The micelles prepared by optimal technology were yellow ，clear and transparent solution ，with good Tyndall effect ；under transmission electron microscope ，they were spherical ，complete and evenly distributed ；the particle size was （15.02±0.16）nm， the PDI was 0.092±0.031，and the Zeta potential was （－6.67±1.47）mV. The CMC of F 127/TPGS was 21 μg/mL，Hyp-F127/ TPGS was stable after 4 weeks of storage at 4 ℃，and the cumulative release rates of Hyp-F 127/TPGS and Hyp control were （66.30±2.93）％（96 h）and（99.24±0.27）％（60 h），respectively. Hyp-F 127/TPGS and Hyp reference were absorbed in each intestinal segment ，and the main absorption sites were jejunum and duodenum respectively ；drug absorption rate constant andapparent absorption coefficient of the former were significantly higher than those of the latter （P＜0.05 or P＜0.01）. E-mail：zhangyuhangxz@163.com CONCLUSIONS The optimized preparation technology of Hyp-F127/TPGS is stable and feasible ；prepared Hyp-F 127/ TPGS shows a sustained -release effect ，which promotes the intestinal absorption of H yp to a certain extent.