Hairy cell leukemia(HCL) is a rare malignant hematological tumor. This article presents the diagnosis and treatment of a patient with recurrent HCL. This patient, a 51-year-old female, was diagnosed with HCL in June 2013 and subsequently received monotherapy with cladribine. Post-treatment evaluation indicated a partial remission. In November 2023, she experienced chest tightness and shortness of breath with ultrasound revealing a right-sided pleural effusion. A follow-up examination in February 2024 confirmed the relapse of HCL. She was then treated with a combination of vemurafenib and rituximab, which resulted in a rapid complete remission without minimal residual disease. This case provides valuable insights into the management of recurrent HCL.

Objective To investigate the relationship between serum kininogen-1(KNG1)and tissue inhib-itor of metalloproteinases-3(TIMP3)levels and coronary artery lesions(CAL)in children with Kawasaki dis-ease(KD)at the acute phase.Methods A total of 132 children with KD at the acute phase admitted to the hospital from October 2020 to February 2024 were selected as the KD group,and were divided into CAL group(66 cases)and non CAL group(66 cases)according to whether they were combined with CAL.In addition,66 healthy children during the same period were selected as the control group.Serum KNG1 and TIMP3 levels were measured using enzyme-linked immunosorbent assay.A multivariate unconditional Logistic regression model with CAL as the dependent variable was established to identify independent factors influencing CAL in children with KD at the acute phase.Receiver operating characteristic(ROC)curve was plotted to evaluate the predictive value of serum N-terminal pro-brain natriuretic peptide(NT-proBNP),KNG1,and TIMP3 levels for CAL in children with KD at the acute phase.Results Compared to the control group,serum KNG1 level was elevated in the KD group(P<0.05),and TIMP3 level was reduced(P<0.05).In the CAL group,serum KNG1 level was higher(P<0.05),and TIMP3 level was lower compared to the non CAL group(P<0.05).Independent risk factors for CAL in children with KD at the acute phase included immunoglobulin resistance(OR=5.618,95%CI:1.203-26.248),increased NT-proBNP level(OR=1.003,95%CI:1.000-1.005),and increased KNG1 level(OR=1.648,95%CI:1.251-2.173),while increased TIMP3 level was identified as an independent protective factor(OR=0.966,95%CI:0.949-0.983,P<0.05).The combination of serum NT-proBNP,KNG1,and TIMP3 levels yielded an area under the curve of 0.930 for predicting CAL in children with KD at the acute phase,which was larger than 0.785,0.801,0.792 of NT-proBNP,KNG1,and TIMP3 a-lone(P<0.05).Conclusion increased level of serum KNG1 and decreased level of TIMP3 are independently associated with the occurrence of CAL in children with KD at the acute phase.The combined prediction of ser-um NT-proBNP,KNG1,and TIMP3 levels has high value in predicting CAL in children with KD at the acute phase.

In fixed prosthodontics, clear exposure of the preparation margin is the prerequisite for obtaining accurate digital impressions and improving the marginal fit of restorations. To resolve the issues associated with the cord retraction technique, such as pain, acute injury, and prolonged procedural time, this study proposes a new technology for intraoral digital impression taking with pneumatic gingival retraction. The new scanning head blows a high-speed airflow that instantaneously separates the free gingiva, locally exposing the subgingival preparation margin. Combined with the farthest point preservation stitching algorithm based on the distance from the normal vector and high-speed laser scanning photography, it achieves global preparation edge data and gingival reconstruction, realizing painless, non-invasive, and efficient precise acquisition of the preparation margin. Using this new technique, a patient with a full porcelain crown restoration on a posterior tooth was treated. The digital impression revealed a clear margin of the preparation, and the crown made from this data has a good marginal fit.

Non-obstructive azoospermia (NOA) is a complex male infertility disease with abnormal spermatogenesis. With the wide application of sequencing technology in recent years, key genes related to spermatogenesis in NOA patients have been continuously mined out. Some epigenetic factors, such as DNA methylation, histone changes and non-coding RNAs are important indicators that reflect to the heterogeneity and complexity of spermatogenesis failure in NOA. This article systematically reviewed the latest studies on the key genes and some epigenetic factors during spermatogenesis of NOA, which may prospectively help to exploit clinical biomarker and to optimize therapeutic proposals.

Non-obstructive azoospermia (NOA) is a complex male infertility disease with abnormal spermatogenesis. With the wide application of sequencing technology in recent years, key genes related to spermatogenesis in NOA patients have been continuously mined out. Some epigenetic factors, such as DNA methylation, histone changes and non-coding RNAs are important indicators that reflect to the heterogeneity and complexity of spermatogenesis failure in NOA. This article systematically reviewed the latest studies on the key genes and some epigenetic factors during spermatogenesis of NOA, which may prospectively help to exploit clinical biomarker and to optimize therapeutic proposals.

Objective:To evaluate the development and application of gastrointestinal endoscopy technology in Beijing-Tianjin-Hebei (BTH) region from 2016 to 2020, and the impact of the corona virus disease 2019 (COVID-19) epidemic on gastrointestinal endoscopy screening and lesion detection rate of medical institutions.Methods:Data of gastroscopy and colonoscopy cases from 26 cooperative institutions in BTH Region Gastrointestinal Endoscopy Medical Association from January 2016 to December 2020 were collected by questionnaire. The number of gastrointestinal endoscopy, the detection of main lesions (including upper gastrointestinal malignant tumors, early gastric cancer and colon cancer), and the number of endoscopic treatment were retrospectively analyzed by year.Results:From 2016 to 2019, the number of gastroscopy and colonoscopy showed a yearly increasing trend with an annual growth rate of over 10%. Compared with 2019, the number of gastroscopy and colonoscopy decreased by 10.86% and 8.29%, respectively, in 2020 due to the impact of the epidemic. The annual detection rates of upper gastrointestinal malignant tumors, early gastric cancer and colon cancer were on a rise, from 7.22%, 1.49% and 8.98% in 2016 to 9.87%, 2.71% and 12.04% in 2020, respectively. The number of gastroscopic mucosal resection, submucosal dissection and colonoscopic endoscopic submucosal dissection increased yearly, from 2 132, 300 and 217 cases in 2016 to 5 466, 872 and 560 cases in 2020, respectively.Conclusion:The Medical Association has promoted the expansion of endoscopic screening and the application of endoscopic treatment techniques, resulting in a continuous increase in the endoscopy detection rate and early cancer diagnosis rate in the BTH region. The sharp decrease of gastrointestinal endoscopy procedures and the increase in the lesion detection rate in 2020 reflect the impact of epidemic COVID-19 on detection of gastrointestinal cancers.

Objective:To explore the change characteristics of event-related potential P300 in different violence risk levels of schizophrenic patients and analyze the risk factors of violence in schizophrenic patients.Methods:Totally 158 schizophrenic patients in Lyuzhou hospital of Shihezi City from January 2019 to August 2020 were collected and assessed with the violence risk scale for 3 days.According to the assessment results, the patients who met the inclusion criteria were divided into low-risk group( n=78), medium-risk group( n=51) and high-risk group( n=29). The auditory P300 of patients in each group was completed within 3 days and act of violence was observed and recorded within one week.Data analysis was carried out by SPSS 20.0 software.The changes of P300 in different violence risk groups were analyzed by ANOVA, and the influencing factors of violence in patients with schizophrenia were analyzed by logistic regression. Results:There was no significant difference in latency of P300 among the three groups (χ 2=4.71, P=0.10), but there was significant difference in amplitude of P300( F=6.67, P<0.01). Compared with the low-risk group ((12.14±9.19) μV), the amplitude of P300 in medium-risk group ((8.25±7.13) μV) and high risk group ((6.71±4.97) μV) decreased significantly ( t=-3.14, -5.45, both P<0.05). There was no significant difference in amplitude of P300 between the high-risk group and the middle-risk group( t=-2.31, P>0.05). The latency and amplitude of schizophrenia patients with violent behavior were significantly different from those without violent behavior ( Z=-6.30, 9.78, both P<0.01). High BVC grade (compared with high-risk group, low-risk group: OR=0.03, 95% CI : 0.00-0.35; the middle risk group: OR=0.09, 95% CI : 0.01-0.62), prolonged P300 latency ( OR=1.30, 95% CI : 1.13-1.48) and decreased P300 amplitude ( OR=0.50, 95% CI: 0.36-0.70), delusion of victimization ( OR=0.12, 95% CI: 0.02-0.76)were the risk factors for violent behavior. Conclusion:The latency and amplitude of P300 can be used as the reliable neuroelectrophysiological indicators for evaluating violence risk in patients with schizophrenia.It has important clinical application value for evaluating violence in patients with schizophrenia.

According to the statistics of new malignant tumors in China in 2015, there were 38 800 female patients under 30 years old, who urgently needed doctors' advices and suggestions on fertility protection and preservation. Ovarian tissue freezing method is the best way to preserve female endocrine function and fertility. At present, the key to the survival of ovarian tissue is to solve the problem of ischemia and reperfusion after ovarian transplantation. In this paper, we will systematically review the mechanism of ischemia and reperfusion after ovarian transplantation, as well as some intervention methods including antioxidants, angiogenesis and remote ischemic preconditioning. This will promote the combination of the latest biotechnology and medical technology, develop more effective, safer and operable solutions to improve the ischemic state after ovarian transplantation, and strive for early clinical use to address the increasing need for female fertility preservation.

Objective:To find a suitable semen treatment method in in vitro fertilization (IVF). Methods:A prospective randomized double-blind design was performed, 300 infertile couples with female factors and no abnormal fertility test were selected for IVF-embryo transfer (ET), the patients were randomly divided into float group (FY group, n=100), density gradient group (DG group, n=100) and upstream group (SU group, n=100). The remaining sperm after fertilization were collected from three groups, and sections of electron microscope were prepared. The ultrastructure of sperm was observed by transmission electron microscope. The differences among the three groups of selected sperm DNA fragment index (DFI) and fertilization rate, cleavage rate, high-quality embryo rate and pregnancy rate during IVF-ET were compared. Results:The sperm DFI of the three groups after treatment was statistically different ( P=0.02), and the sperm DFI of the FY group was significantly lower than that of DG group and SU group (3.22%±2.73% vs. 8.31%±2.14% vs. 6.43%±2.56%). The rates of sperm head and tail plasma membrane integrity rate in FY group were significantly higher than those in the other two groups (92.0%±24.2% vs. 80.2%±29.5% vs. 73.2%±30.1% and 93.9%±1.2% vs. 80.1%±1.1% vs. 74.9%±1.2%), and the differences were statistically significant ( P=0.01, P=0.03). The high-quality embryo rate and the blastocyst formation rate of FY group were significantly higher than those of DG group and SU group [44.14% (452/1024) vs. 32.30% (292/904) vs. 32.46% (296/912) and 54.40% (396/728) vs. 43.52% (302/694) vs. 46.34% (330/712)], and the differences were statistically significant (all P<0.001). The pregnancy rate and the implantation rate in the fresh cycle FY group were significantly higher than those in DG and SU groups [57.14% (24/42) vs. 33.33% (16/48) vs. 35.56% (16/45) and 50.00% (30/60) vs. 27.45% (14/51) vs. 28.26% (13/46)], and the differences were statistically significant ( P=0.04, P=0.02). The pregnancy rate and implantation rate in the resuscitation cycle FY group were significantly higher than those in the DG and SU groups [52.38% (22/42) vs. 31.25% (10/32) vs. 37.14% (13/35) and 52.38% (22/42) vs. 29.41% (10/34) vs. 30.56% (11/36)], and the difference was statistically significant ( P=0.03, P=0.02). Conclusion:The Plankton method can reduce physical damage to sperm, improve the integrity rate of sperm plasma membrane and decrease sperm DFI, improve assisted reproductive technology outcome.

According to the statistics of new malignant tumors in China in 2015, there were 38 800 female patients under 30 years old, who urgently needed doctors' advices and suggestions on fertility protection and preservation. Ovarian tissue freezing method is the best way to preserve female endocrine function and fertility. At present, the key to the survival of ovarian tissue is to solve the problem of ischemia and reperfusion after ovarian transplantation. In this paper, we will systematically review the mechanism of ischemia and reperfusion after ovarian transplantation, as well as some intervention methods including antioxidants, angiogenesis and remote ischemic preconditioning. This will promote the combination of the latest biotechnology and medical technology, develop more effective, safer and operable solutions to improve the ischemic state after ovarian transplantation, and strive for early clinical use to address the increasing need for female fertility preservation.