Objective To explore the predictive value of combined detection of serum C-peptide(C-P),forkhead box O transcription factor-3a(FOXO3a),and metallothionein(Metrnl)for diabetic peripheral neuropathy(DPN).Methods A total of 210 patients with type 2 diabetes mellitus(T2DM)in Kailuan General Hospital were enrolled in this study from May 2021 to May 2023.All the patients were followed up for 1 year and 4 patients lost follow-up.All the 206 patients were divided into DPN group(n=52)and simple T2DM group(n=154)according to whether they had concurrent DPN.Baseline data,serum C-P,FOXO3a,and Metrnl levels were compared between the two groups.The relationship between serum C-P,FOXO3a,Metrnl levels and DPN was analyzed using point-biserial correlation and Cox regression analysis.The predictive value of serum C-P,FOXO3a,and Metrnl levels for DPN was analyzed using receiver operating characteristic(ROC)curve analysis.Results DM duration,hemoglobin A1c(HbA1c)levels and FOXO3a were higher in the DPN group than in the T2DM group(P<0.05).Serum C-P and Metrnl levels were lower in the DPN group than in the T2DM group(P<0.05).Correlation analysis showed that serum C-P and Metrnl levels were negatively correlated with DPN(P<0.01),while FOXO3a levels were positively correlated with DPN(P<0.01).Cox regression analysis revealed that serum C-P,FOXO3a,and Metrnl were independent factors for DPN,both before and after adjusting DM duration and HbA1c.The area under the curves for predicting DPN using serum C-P,FOXO3a,and Metrnl were 0.773,0.788,and 0.756,respectively.The sensitivities were 75.00%,76.92%,and 76.92%,and the specificities were 70.78%,69.48%,and 67.53%.The AUC for predicting DPN using the combination of the three markers was 0.921,with a sensitivity of 80.77%and a specificity of 90.26%.The combined predictive value was significantly higher than that of each marker alone(Z=3.520,2.969,3.531,all P<0.05).Conclusions Serum C-P,FOXO3a,and Metrnl are independent influencing factors for DPN in T2DM patients,and the combined detection has reliable predictive value.

Objective To explore the predictive value of combined detection of serum C-peptide(C-P),forkhead box O transcription factor-3a(FOXO3a),and metallothionein(Metrnl)for diabetic peripheral neuropathy(DPN).Methods A total of 210 patients with type 2 diabetes mellitus(T2DM)in Kailuan General Hospital were enrolled in this study from May 2021 to May 2023.All the patients were followed up for 1 year and 4 patients lost follow-up.All the 206 patients were divided into DPN group(n=52)and simple T2DM group(n=154)according to whether they had concurrent DPN.Baseline data,serum C-P,FOXO3a,and Metrnl levels were compared between the two groups.The relationship between serum C-P,FOXO3a,Metrnl levels and DPN was analyzed using point-biserial correlation and Cox regression analysis.The predictive value of serum C-P,FOXO3a,and Metrnl levels for DPN was analyzed using receiver operating characteristic(ROC)curve analysis.Results DM duration,hemoglobin A1c(HbA1c)levels and FOXO3a were higher in the DPN group than in the T2DM group(P<0.05).Serum C-P and Metrnl levels were lower in the DPN group than in the T2DM group(P<0.05).Correlation analysis showed that serum C-P and Metrnl levels were negatively correlated with DPN(P<0.01),while FOXO3a levels were positively correlated with DPN(P<0.01).Cox regression analysis revealed that serum C-P,FOXO3a,and Metrnl were independent factors for DPN,both before and after adjusting DM duration and HbA1c.The area under the curves for predicting DPN using serum C-P,FOXO3a,and Metrnl were 0.773,0.788,and 0.756,respectively.The sensitivities were 75.00%,76.92%,and 76.92%,and the specificities were 70.78%,69.48%,and 67.53%.The AUC for predicting DPN using the combination of the three markers was 0.921,with a sensitivity of 80.77%and a specificity of 90.26%.The combined predictive value was significantly higher than that of each marker alone(Z=3.520,2.969,3.531,all P<0.05).Conclusions Serum C-P,FOXO3a,and Metrnl are independent influencing factors for DPN in T2DM patients,and the combined detection has reliable predictive value.

Objective To investigate the protein expression of stanniocalcin 1 (STC1) in papillary thyroid carcinoma (PTC) in gerontal patients versus non-gerontal subjects,and its relationship with clinicopathological features.Methods The protein expression levels of STC1 in PTC,nodular goiter and normal thyroid tissues in the gerontal patients versus non-gerontal subjects were detected by immunohistochemistry.The protein expressions of STC1 were detected by Western blotting.Results The protein expression of STC1 in gerontal patients was higher in PTC than in nodular goiter and normal thyroid tissues (60.9％ vs.30.0％,15.0％,P＜0.05 or 0.01).The relative expression of STC1 protein in gerontal patients was higher in PTC than in nodular goiter and normal thyroid tissues [(0.647 ± 0.076) vs.(0.280 ± 0.039),(0.248 ± 0.065),F =9.965 and 1.143,both P＜0.01].STC1 protein expression had no correlations with age,gender,tumor diameter and tumor position in patients (P＜0.05),while it was associated with tumor lymph node metastasis and clinical stage (P＜0.05).There was no significant difference in above indexes between the elderly and non-elderly patients with papillary thyroid carcinoma (F=0.007,P=0.934).Conclusions STC1 protein may be associated with the development of papillary thyroid carcinoma,and it may has a some reference value in differentiating benign from malignant thyroid neoplasms and in predicting the prognosis of thyroid carcinomas.

Objective To investigate the association of serum dehydroepiandrosterone sulfate( DHEA-S) with the cognition in male type 2 diabetes mellitus(T2DM)patients. Methods 99 male patients cared at Tangshan Gongren Hospital and another 97 male healthy controls without T2DM from the medical examination center were recruited. Data on demographic characteristics and clinical parameters were collected, DHEA-S was measured by radioimmunologic assay. Cognitive performance was assessed by the Repeatable Battery for the Assessment of Neuropsychological Status(RBANS). Results (1) Serum DHEA-S levels were lower in male T2DM patients than that of controls[(2. 66 ± 0. 78 vs 4. 02 ± 1. 24) μmol/ L, P<0. 01];(2) Compared with controls, RBANS scores including immediate memory(79. 24 ± 17. 47 vs 86. 25 ± 15. 21, P<0. 01), visuospatial/ constructional(83. 98 ± 17. 98 vs 97. 24 ± 11. 51, P<0. 01), attention(96. 04 ± 14. 65 vs 101. 45 ± 13. 93, P<0. 01), delayed memory (89. 28 ± 11. 74 vs 97. 41 ± 9. 41, P<0. 01), and total scores(85. 85 ± 11. 46 vs 94. 60 ± 10. 91, P<0. 01)were all lower in male T2DM patients;(3) RBANS scores including delayed memory(84. 53 ± 12. 23 vs 93. 94 ± 9. 18, P<0. 01)and total scores(80. 33 ± 10. 91 vs 91. 26 ± 9. 25, P<0. 01)in T2DM patients with low-level DHEA-S(DHEA-S-L)were all lower than those of patients with high-level DHEA-S;(4)Male T2DM patients with cognitive impairment had lower levels of DHEA-S than patients without cognitive impairment(2. 31 ±0. 79 对 2. 90 ±0. 67, P<0. 01);(5) In male T2DM patients, DHEA-S was positively correlated with delayed memory(r = 0. 252, P = 0. 019) and total scores(r=0. 258, P= 0. 016). Conclusion Male T2DM patients are with lower serum DHEA-S levels and worse cognitive performance, and serum DHEA-S was positively correlated with their cognitive performance, suggesting that serum DHEA-S may be involved in the cognitive deficits of male T2DM patients.