Loss-of-function variants in CSDE1 have been strongly linked to neuropsychiatric disorders, yet the precise role of CSDE1 in neurogenesis remains elusive. In this study, we demonstrate that knockout of Csde1 during cortical development in mice results in impaired neural progenitor proliferation, leading to abnormal cortical lamination and embryonic lethality. Transcriptomic analysis revealed that Csde1 upregulates the transcription of genes involved in the cell cycle network. Applying a dual thymidine-labelling approach, we further revealed prolonged cell cycle durations of neuronal progenitors in Csde1-knockout mice, with a notable extension of the G1 phase. Intersection with CLIP-seq data demonstrated that Csde1 binds to the 3' untranslated region (UTR) of mRNA transcripts encoding cell cycle genes. Particularly, we uncovered that Csde1 directly binds to the 3' UTR of mRNA transcripts encoding Cdk6, a pivotal gene in regulating the transition from the G1 to S phases of the cell cycle, thereby maintaining its stability. Collectively, this study elucidates Csde1 as a novel regulator of Cdk6, sheds new light on its critical roles in orchestrating brain development, and underscores how mutations in Csde1 may contribute to the pathogenesis of neuropsychiatric disorders.
Animals ; Neurogenesis/genetics* ; Cell Cycle/genetics* ; Mice, Knockout ; Mice ; Neural Stem Cells/metabolism* ; DNA-Binding Proteins/metabolism* ; Cyclin-Dependent Kinase 6/genetics* ; Cell Proliferation ; 3' Untranslated Regions ; Cerebral Cortex/embryology* ; RNA-Binding Proteins ; Mice, Inbred C57BL

Objective To explore the effect of time axis management method on emergency care of patients with acute my-ocardial infarction(AMI).Methods A total of 60 AMI patients admitted to the emergency department from June to December 2022 were assigned to the control group,whereas 62 AMI patients admitted from January to June 2023 were set as the observation group.The control group received routine emergency treatment,while the observation group received time-axis management inter-vention.The two groups were compared in terms of treatment timelines,clinical efficiency,and patient satisfaction rates.Results The observation group demonstrated significantly shorter time metrics compared to the control group(P＜0.05),including ambu-lance response time,hospitalization duration,triage time,diagnostic testing time,and emergency waiting time.The observation group also showed higher rescue success rates(P＜0.05).Additionally,the observation group exhibited significantly higher satis-faction rates with ambulance response time,emergency triage flowchart,auxiliary examination duration,registration fee charging,medical treatment scheduling,waiting environment,and nursing service quality(all P＜0.05).Conclusion The time axis man-agement method can effectively shorten rescue time for AMI patients,enhance rescue efficiency,and improves patient satisfaction.

With the widespread application of nuclear technology,the prevention and treatment of radiation damage have become significant issues in the fields of public health and medicine.This article reviews the adverse drug reactions and their monitoring for radioprotective drugs,and discusses their post-marketing re-evaluation in terms of safety,efficacy,economics,and quality standards.Research indicates that while there is a wide variety of radioprotective drugs,safety concerns are prevalent.Future efforts should focus on strengthening the monitoring and management of adverse drug reactions,optimizing drug safety,and exploring the potential of combined drug applications through multidisciplinary collaboration to enhance anti-radiation efficacy.

Objective To explore the effect of time axis management method on emergency care of patients with acute my-ocardial infarction(AMI).Methods A total of 60 AMI patients admitted to the emergency department from June to December 2022 were assigned to the control group,whereas 62 AMI patients admitted from January to June 2023 were set as the observation group.The control group received routine emergency treatment,while the observation group received time-axis management inter-vention.The two groups were compared in terms of treatment timelines,clinical efficiency,and patient satisfaction rates.Results The observation group demonstrated significantly shorter time metrics compared to the control group(P＜0.05),including ambu-lance response time,hospitalization duration,triage time,diagnostic testing time,and emergency waiting time.The observation group also showed higher rescue success rates(P＜0.05).Additionally,the observation group exhibited significantly higher satis-faction rates with ambulance response time,emergency triage flowchart,auxiliary examination duration,registration fee charging,medical treatment scheduling,waiting environment,and nursing service quality(all P＜0.05).Conclusion The time axis man-agement method can effectively shorten rescue time for AMI patients,enhance rescue efficiency,and improves patient satisfaction.

Objective:To retrieve, evaluate and summarize evidence on dietary management in adults with prediabetes, and to provide an evidence-based basis for clinical nursing practice and patient self-management.Methods:Guidelines, expert consensus, clinical decisions, evidence summaries, and systematic reviews on dietary management of patients with prediabetes were electronically searched on domestic and international guideline websites, association websites, and databases in both Chinese and English. The search period was from database establishment to October 31, 2024. Quality assessment of the literature and extraction and summarization of the evidence was carried out.Results:A total of 17 papers were included, including six guidelines, three expert consensus, and eight systematic reviews and Meta-analysis. A total of 39 pieces of evidence were summarized, including eight themes of total energy intake; nutrient recommendations, cooking principles; dietary pattern selection; dietary supplement recommendations; food recommendations; synergy of diet, exercise and medication; and dietary guidance.Conclusions:The best evidence summarized can provide reference for clinical medical and nursing staff, patients with prediabetes and their families. Future evidence-based translational practice for dietary management in prediabetic patients according to this evidence summary should be based on clinical context and consideration of the individual patient's situation for dietary program development.

Objective:To retrieve, evaluate and summarize evidence on dietary management in adults with prediabetes, and to provide an evidence-based basis for clinical nursing practice and patient self-management.Methods:Guidelines, expert consensus, clinical decisions, evidence summaries, and systematic reviews on dietary management of patients with prediabetes were electronically searched on domestic and international guideline websites, association websites, and databases in both Chinese and English. The search period was from database establishment to October 31, 2024. Quality assessment of the literature and extraction and summarization of the evidence was carried out.Results:A total of 17 papers were included, including six guidelines, three expert consensus, and eight systematic reviews and Meta-analysis. A total of 39 pieces of evidence were summarized, including eight themes of total energy intake; nutrient recommendations, cooking principles; dietary pattern selection; dietary supplement recommendations; food recommendations; synergy of diet, exercise and medication; and dietary guidance.Conclusions:The best evidence summarized can provide reference for clinical medical and nursing staff, patients with prediabetes and their families. Future evidence-based translational practice for dietary management in prediabetic patients according to this evidence summary should be based on clinical context and consideration of the individual patient's situation for dietary program development.

With the widespread application of nuclear technology,the prevention and treatment of radiation damage have become significant issues in the fields of public health and medicine.This article reviews the adverse drug reactions and their monitoring for radioprotective drugs,and discusses their post-marketing re-evaluation in terms of safety,efficacy,economics,and quality standards.Research indicates that while there is a wide variety of radioprotective drugs,safety concerns are prevalent.Future efforts should focus on strengthening the monitoring and management of adverse drug reactions,optimizing drug safety,and exploring the potential of combined drug applications through multidisciplinary collaboration to enhance anti-radiation efficacy.

Objective To observe the effects of folic acid(FA)supplementation on the expression of Flotillin-1 and β-amyloid protein(Aβ)-metabolism-related proteins in the brains of inflammation-stimulated Alzheimer's disease(AD)mice.Methods Twenty-seven 6-month-old male APP/PS1 mice were randomly divided into AD,AD+LPS,and AD+LPS+FA groups,with nine mice in each group.Nine C57BL/6J male mice born within the same month were used as the Control group.The AD+LPS+FA group was given folic-acid-supplemented feed(8 mg/kg)for 3 months of intervention,while the other three groups were fed normal feed.Lipopolysaccharide solution(LPS,250 μg/(kg·d))was injected intraperitoneally into mice in the AD+LPS and AD+LPS+FA groups 1 week before the end of the experiment,and saline was injected into the remaining two groups.The serum inflammatory factors TNF-α and IL-6 levels and brain tissue Aβ1-40 and Aβ1-42 levels of mice in each group were detected by ELISA.Flotillin-1 protein expression in brain tissue was detected using Western blot,and the co-expression of Flotillin-1 and Aβ1-42/APP/PS1/BACE1 in the cortical region of the brain was detected via immunofluorescence double-labeling.Results After ANOVA analysis,we found mice in the AD group had elevated serum TNF-α and IL-6 levels(P＜0.05),elevated levels of Aβ1-40 and Aβ1-42(P＜0.05),increased expression of Flotillin-1 protein(P＜0.05),and increased co-expression of Flotillin-1 and Aβ1-42/APP/PS1/BACE1 in the cortical brain tissue(P＜0.05)compared with the Control group.Compared with mice in the AD group,those in the AD+LPS group had further increases in serum inflammatory factors and Aβ levels in the brain(P＜0.05)and increased co-expression of Flotillin-1 and Aβ1-42/APP/BACE1 double-labeled proteins in their cortical brain tissue(P＜0.05).Compared with mice in the AD+LPS group,those in the AD+LPS+FA group had lower in vivo inflammation levels and Aβ content in the brain(P＜0.05),lower brain tissue Flotillin-1 protein expression(P＜0.05),and lower Flotillin-1 and Aβ1-42/APP/PS1/BACE1 protein co-expression in cortical brain tissue(P＜0.05).Conclusions Folic acid supplementation may reduce Flotillin-1 protein expression and Aβ deposition in the brain of AD inflammatory mice.

Polycyclic aromatic hydrocarbons (PAHs) are a class of persistent pollutants that are widely distributed in the environment. Due to their stable structure and poor degradability, PAHs exhibit carcinogenic, teratogenic, and mutagenic toxicity to the ecological environment and organisms, thus increasing attentions have been paid to their removals and remediation. Green, safe and economical technologies are widely used in the bioremediation of PAHs-contaminated soil. This article summarizes the present status of PAHs pollution in soil of China from the aspects of origin, migration, fate, and pollution level. Meanwhile, the types of microorganisms and plants capable of degrading PAHs, as well as the underlying mechanisms, are summarized. The features of three major bioremediation technologies, i.e., microbial remediation, phytoremediation, and joint remediation, are compared. Analysis of the interaction mechanisms between plants and microorganisms, selection and cultivation of stress-resistant strains and plants, as well as safety and efficacy evaluation of practical applications, are expected to become future directions in this field.
Biodegradation, Environmental ; Polycyclic Aromatic Hydrocarbons/toxicity* ; Soil ; Soil Microbiology ; Soil Pollutants

The gut microbiota is profoundly involved in glucose and lipid metabolism, in part by regulating bile acid (BA) metabolism and affecting multiple BA-receptor signaling pathways. BAs are synthesized in the liver by multi-step reactions catalyzed via two distinct routes, the classical pathway (producing the 12α-hydroxylated primary BA, cholic acid), and the alternative pathway (producing the non-12α-hydroxylated primary BA, chenodeoxycholic acid). BA synthesis and excretion is a major pathway of cholesterol and lipid catabolism, and thus, is implicated in a variety of metabolic diseases including obesity, insulin resistance, and nonalcoholic fatty liver disease. Additionally, both oxysterols and BAs function as signaling molecules that activate multiple nuclear and membrane receptor-mediated signaling pathways in various tissues, regulating glucose, lipid homeostasis, inflammation, and energy expenditure. Modulating BA synthesis and composition to regulate BA signaling is an interesting and novel direction for developing therapies for metabolic disease. In this review, we summarize the most recent findings on the role of BA synthetic pathways, with a focus on the role of the alternative pathway, which has been under-investigated, in treating hyperglycemia and fatty liver disease. We also discuss future perspectives to develop promising pharmacological strategies targeting the alternative BA synthetic pathway for the treatment of metabolic diseases.