Loss-of-function variants in CSDE1 have been strongly linked to neuropsychiatric disorders, yet the precise role of CSDE1 in neurogenesis remains elusive. In this study, we demonstrate that knockout of Csde1 during cortical development in mice results in impaired neural progenitor proliferation, leading to abnormal cortical lamination and embryonic lethality. Transcriptomic analysis revealed that Csde1 upregulates the transcription of genes involved in the cell cycle network. Applying a dual thymidine-labelling approach, we further revealed prolonged cell cycle durations of neuronal progenitors in Csde1-knockout mice, with a notable extension of the G1 phase. Intersection with CLIP-seq data demonstrated that Csde1 binds to the 3' untranslated region (UTR) of mRNA transcripts encoding cell cycle genes. Particularly, we uncovered that Csde1 directly binds to the 3' UTR of mRNA transcripts encoding Cdk6, a pivotal gene in regulating the transition from the G1 to S phases of the cell cycle, thereby maintaining its stability. Collectively, this study elucidates Csde1 as a novel regulator of Cdk6, sheds new light on its critical roles in orchestrating brain development, and underscores how mutations in Csde1 may contribute to the pathogenesis of neuropsychiatric disorders.
Animals ; Neurogenesis/genetics* ; Cell Cycle/genetics* ; Mice, Knockout ; Mice ; Neural Stem Cells/metabolism* ; DNA-Binding Proteins/metabolism* ; Cyclin-Dependent Kinase 6/genetics* ; Cell Proliferation ; 3' Untranslated Regions ; Cerebral Cortex/embryology* ; RNA-Binding Proteins ; Mice, Inbred C57BL

Objective To analyze the unqualified air reasons of laminar flow clean operation room when monitored,to strengthen the prevention and make sure static air of the laminar flow clean operating room air 100％ qualified.Methods Each two of the 100 and 1 000 class laminar flow operation room had unqualified air before November 2013,and our department tried to find the reasons and countermeasures.From December 2013 to February 2014,we monitored air four times after carrying out countermeasures,and compared the qualified rate of operation room air with the air qualified rate before countermeasures used.Results The infection rate of Ⅰ type operative incision were always below 0.2％ in our hospital.After comprehensive improvement of operation room,the air qualified rate was beyond the rate of before improvement 85.7％ with statistical significance (P ＜ 0.05).Conclusions It can increase air qualified rate of laminar flow clean operation room by the operational management,cleaning management,aseptic management for maintaining operation room clean system and the training of air sampling personnel.