Objective To study the current status of the randomized controlled trial(RCT)outcome indicators of acupuncture and moxibustion in the treatment of chronic fatigue syndrome(CFS);To provide guidance and references for clinical research and protocol design.Methods RCT of acupuncture and moxibustion for CFS were retrieved from CNKI,Wanfang Data,VIP,SinoMed,PubMed,Embase,Web of Science databases from January 1,2015 to December 9,2024.Through literature screening and data extraction,Excel 2019 was used to make a descriptive analysis of the basic characteristics,diagnostic criteria,intervention measures,classification of outcome indicators,use frequency and evaluation time points of the included studies.Origin2021 software was used to draw bubble charts for visual display.Results Totally 119 RCT were included,involving 8 445 subjects,5 diagnostic criteria of Western medicine,5 diagnostic criteria of TCM,and 6 outcome indicators,namely clinical symptoms/signs,symptoms/syndrome of TCM,physical and chemical examination,quality of life,safety evaluation,and patient satisfaction.A total of 58 outcome indicators were reported,with a cumulative frequency of 430 times.Conclusion The RCT of acupuncture and moxibustion treatment of CFS have some problems,such as incomplete baseline assessment,lack of sample size calculation basis,high risk of bias,ignorance of primary and secondary indicators in the selection of outcome indicators,numerous indicators selection and measurement points,lack of long-term follow-up,inconsistent evaluation criteria for TCM syndrome efficacy,and insufficient attention to health economics evaluation and safety outcome indicators.

Objective To study the current status of the randomized controlled trial(RCT)outcome indicators of acupuncture and moxibustion in the treatment of chronic fatigue syndrome(CFS);To provide guidance and references for clinical research and protocol design.Methods RCT of acupuncture and moxibustion for CFS were retrieved from CNKI,Wanfang Data,VIP,SinoMed,PubMed,Embase,Web of Science databases from January 1,2015 to December 9,2024.Through literature screening and data extraction,Excel 2019 was used to make a descriptive analysis of the basic characteristics,diagnostic criteria,intervention measures,classification of outcome indicators,use frequency and evaluation time points of the included studies.Origin2021 software was used to draw bubble charts for visual display.Results Totally 119 RCT were included,involving 8 445 subjects,5 diagnostic criteria of Western medicine,5 diagnostic criteria of TCM,and 6 outcome indicators,namely clinical symptoms/signs,symptoms/syndrome of TCM,physical and chemical examination,quality of life,safety evaluation,and patient satisfaction.A total of 58 outcome indicators were reported,with a cumulative frequency of 430 times.Conclusion The RCT of acupuncture and moxibustion treatment of CFS have some problems,such as incomplete baseline assessment,lack of sample size calculation basis,high risk of bias,ignorance of primary and secondary indicators in the selection of outcome indicators,numerous indicators selection and measurement points,lack of long-term follow-up,inconsistent evaluation criteria for TCM syndrome efficacy,and insufficient attention to health economics evaluation and safety outcome indicators.

Objective: To investigate the effects of over expression and low expression of antisense transcripts of circular RNA cerebellar degeneration associated protein 1 (CDR1as) in Balb/C mouse bone marrow mesenchymal stem cells (BMSCs) on factors related to osteogenesis and angiogenesis. Methods: BMSCs were cultured and identified in vitro. The lentiviral (LV) vector containing the overexpressed and silenced circRNA CDR1as genes and the control lentivirus were respectively transfected into mouse BMSCs, and stable cell lines were screened. The cells were divided into the circRNACDR1as over expression group and the over expression control group, and the CircRNACDR1as low expression group and the low expression control group. The components were stained with Alizarin Red S and alkaline phosphatase after 14 and 21 days of osteoinduction; qRT-PCR was used to detect the target genes circRNA CDR1as, osteogenic differentiation markers alkaline phosphatase (ALP), runt- related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN), osterix(Osx), collagen I (COL-1), and the mRNA expression levels of vascular endothelial grown factor (VEGF) and angiogenin-1 (Ang-1). Results: The results of alizarin red staining and alkaline phosphatase staining showed that the extracellular matrix calcium precipitation and ALP staining area of the over expression experimental group was greater than its control group, and those of the low expression experimental group was less than its control group. As the number of days of osteogenic induction increased, the calcium precipitation and ALP staining in each group also increased. RT-PCR results showed that the mRNA expression levels of circRNA CDR1as, ALP, RUNX2, OCN, OPN, OSX, COL-1, VEGF and Ang-1 in the over expression experimental group BMSCs were significantly increased (P<0.001). In the low expression experimental group, the mRNA expression levels of circRNA CDR1as, ALP, RUNX2, OCN, OPN, OSX, COL-1, VEGF and Ang-1 in BMSCs were significantly reduced (P<0.001). Conclusion Over expression of the circRNA CDR1as gene promotes the osteogenic differentiation and angiogenesis of BMSCs. Low expression of the circRNA CDR1as gene inhibits the osteogenic differentiation and angiogenesis of BMSCs.

Objective To investigate the current status and influencing factors of job burnout and self efficacy of midwives in Beijing, and to explore the correlation between job burnout and self efficacy in midwives. Methods We selected 335 midwives in wards of obstetrics department from 20 hospitals ( 13 general hospitals+ 7 women-children special hospitals ) in Beijing as respondents by convenient sampling. And then, we investigated the job burnout, self efficacy and the influencing factors. Results The total average score of job burnout of 335 midwives was (70.0±16.1). There were 5 (1.5%) with zero burnout, 73 (21.8%) with mild burnout, 178 (53.1%) with moderate burnout, 79 (23.6%) with severe burnout among 335 midwives. The total average score of self efficacy of 335 midwives was (26.4±5.9). The major influencing factors of job burnout and self efficacy were the age, working hours in clinic, working hours in delivery room (P<0.05). The regression showed that there was significant correlation between each dimension of job burnout and self efficacy. Conclusions The degree of job burnout is high and the self efficacy is the medium level in the midwife. The effective measures should be taken to enhance the midwives′self efficacy and improve their job burnout.

Objective To investigate midwives human resource and accouched delivery mode, and analyze the midwives personnel structure and manning level status, so as to provide reference for the construction and reasonable allocation of midwifery team. Methods Self-designed scale was used to comprehend accouched delivery mode and midwives human resource status in 20 hospitals which owned obstetric room and were selected by convenient sampling method form May to June 2014 in Beijing, and the results of scale were analyzed and compared. Results The characteristics of midwifery team were almost aged from 25 to 34 (59. 5%), and highest education levels were associated degree (50. 2%) and bachelor degree (41. 7%), and non professional midwifery graduation was 62. 6%, and primary occupational title accounting for 69. 8%. The accompanied delivery rate in 20 hospitals was 90. 0%, 72. 2% of labor was whole process companion, and 27. 8% people chose to accompany at first or second labor stage. 45% one shift of midwives lasted 12 to 16 h, and just 25% of them was 8 h working length for one shift, and 20% of them worked lasted 24 h, and 10% of midwives had uncertain length work shift. Different accouched delivery mode had similar midwifery allocation, and one delivery bed averagely disposed ( 3. 75 ± 1. 21 ) midwives, and one midwife should take responsibility for (137. 92 ± 73. 09) neonatus in one year. Conclusions The educational level and occupational title of midwives require to improve in Beijing region, the midwives proportioning should research and allocate combined with accouched delivery mode and woke load. Nursing management and training tutor should actively explore and regulate midwives continuing education and training system bonded with national situation.