Objective To study the chemical constituents from the herbs of Dendrobium crepidatum and identify its antitumor bioactive compounds.Methods The constituents were extracted by methyl alcohol and isolated by CC and Sephadex LH-20.Their structures were identified by spectroscopic analysis(1H NMR,13CNMR and EIMS).The antitumor activity of the constituents was determined by MTT assay in vitro.Results 9 compouds were obtained and identified as p-hydroxybenzaldehyde(1),p-methoxyphenol(2),p-Hydroxybenzyl alcohol(3),hydroquinone(4),(24R)-ethylcholest-5-en-3-ol-7-one(5),sinalexin(6),bis(2-ethylhexyl)benzene-1,2-dicarboxylate(7),pinoresinol(8),Chaenomin B(9).The antitumor activity of compounds 5～9 was tested,and it was found that compound 9 had good inhibitory activity against A549 cell line.Conclusion Compounds 2～5,7～8 were isolated from the herbs for the first time and compounds 6,9 were isolated from this genus for the first time.IC50 of 9 on A549 cell line is 29.35 μM.

Objective To observe clinical effects of the modified nasopharyngeal airway tube combined with intubation soft lens in endotracheal intubation.Methods Two hundred patients who were to receive surgery were chosen as research subjects.With the knowledge and consent of the patients and following the signing of the letter of agreement,the patients were divided into 4 groups:group A,B,C and D,each consisting of 50 patients.For the patients of group A and B,endotracheal intubation was performed by high-qualified anesthesiologists (with the certificate of medical practitioner for over 5 years),while for the patients of group C and D,intubation was done by low-qualified ones (with the certificate of medical practitioner for less than 5 years).For the patients of group A and C,nasopharyngeal airway tube was placed through the approach of side nasal cavity following anesthesia induction,and then soft lens was used to complete endotracheal intubation via nasopharyngeal airway.For the patients of group B and D,soft lens intubation was performed directly through nasal cavity following anesthesia induction.Medical data,such as intubation time,number of intubation,first time of glottis exposure,cases of intubation failure,and adverse reactions or complications induced by intubation were recorded for further analysis.Results The intubation time of group A was shorter than that of group 13,and the time of group C was shorter than that of group D,and statistical significance could be seen,when comparisons were made between them(P ＜ 0.05).The success rate at the first try of intubation for group A and C were respectively 84％ and 82％,and the failure rate was all 2％,while for group B and D the success rate of intubation were respectively 50％ and 30％,and failure rates were respectively 10％ and 20％.Statistical significance could be noticed,when comparisons were made between the groups,i.e.between group A and B,between group C and D and also between group B and D (P ＜ 0.05).Conclusion Modified nasopharyngeal airway tube combined with intubation soft lens could obviously shorten endotracheal intubation time and increase the success rate of intubation.No significant differences could be noted in intubation time and failure rate,when the use of modified nasopharyngeal airway tube was combined with intubation soft lens by high-qualified and low-qualified anesthesiologists.