Panax ginseng as a perennial herb of Araliaceae, exhibits pharmacological effects such as central nervous system stimulation, anti-tumor properties, and cardiovascular and cerebrovascular protection. The B3 gene family plays a crucial role in growth and development, antioxidant activity, stress resistance, and secondary metabolism regulation of plants and has been extensively studied in various plants. However, the identification and analysis of the B3 gene family in P. ginseng have not been reported. In this study, a total of 145 B3 genes(PgB3s) with complete open reading frames(ORF) were identified from P. ginseng and classified into five subfamilies based on domain types. Through correlation analysis with ginsenoside content, SNP/InDels analysis, and interaction analysis with key enzyme genes, 15 PgB3 transcripts were found to be significantly correlated with ginsenoside content and exhibited a close interaction network with key enzyme genes involved in ginsenoside biosynthesis, which indicated that these genes may participate in the regulation of ginsenoside biosynthesis. Additionally, this study found that PgB3 genes exhibited induced expression in response to methyl jasmonate(MeJA) stress, which aligned with the presence of abundant stress response elements in their promoters, confirming the important role of the B3 gene family in P. ginseng in stress resistance. The results of this study revealed the potential functions of PgB3 genes in ginsenoside biosynthesis and stress response, providing a significant theoretical basis for further research on the functions of PgB3 genes and their regulatory mechanisms.
Panax/metabolism* ; Gene Expression Regulation, Plant ; Plant Proteins/metabolism* ; Ginsenosides/biosynthesis* ; Multigene Family ; Phylogeny

Objective To investigate the current situation of the ventilator use and management in secondary and tertiary medical institutions in Sichuan Province to provide references for management and training of ventilator users.Methods A questionnaire survey involving in 235 nurses from 13 secondary and tertiary medical institutions in Sichuan Province was conducted in terms of general information of the respondents,pipeline connection and parameter setup of ventilators,cleaning,sterilization and maintenance of ventilators,identification and treatment of ventilator alarms and hospital-acquired infection prevention and control.Results The results showed 20.4％of the respondents were nurses from critical care departments,and 79.6％of the respondents underwent the training for using the ventilator in their department;91.9％of the respondents could conduct ventilator self-testing by connecting the simulated lungs after the ventilator was switched on;66.4％of the respondents indicated that the initial parameters of the ventilator were set by the doctor;60.9％of the respondents proved that the performance monitoring and routine maintenance of the ventilator were carried out by the nurse;63.4％of the respondents showed disposable lines were commonly used in the departments.There were 89.8％of the respondents said the daily sterilization and management of the ventilator were performed by the nurse;46.0％of the respondents expressed the external surface of the ventilator was disinfected mainly with gamma disinfectant wipes;44.7％of the respondents indicated the external surface of the ventilator was sterilized every day;48.5％of the respondents said the internal airway of the ventilator was disinfected;57.0％of the respondents proved the disinfection was conducted after the expiratory flow sensors were used by any patient.There were 74.5％of the respondents that had paid attention to the ventilator waveform,and by the ventilator waveform only 21.3％were correct in determining whether there was secretion or fluid in the circuit and 22.1％in clarifying whether there was air leakage in the circuit.There were 59.1％of the respondents indicated that the closed suction tube was replaced once every 24 h;71.1％of the respondents could perform airbag pressure monitoring by the special pressure gauge.Conclusion Most of the nurses from secondary and tertiary medical institutions in Sichuan Province can use ventilators correctly,who have problems in disinfection specifications,infection prevention and control or recognition of ventilation waveform.It's suggested the training be strengthened to enhance the clinical nursing staffs in ventilator management.[Chinese Medical Equipment Journal,2025,46(3):75-80]

Objective To investigate the current situation of the ventilator use and management in secondary and tertiary medical institutions in Sichuan Province to provide references for management and training of ventilator users.Methods A questionnaire survey involving in 235 nurses from 13 secondary and tertiary medical institutions in Sichuan Province was conducted in terms of general information of the respondents,pipeline connection and parameter setup of ventilators,cleaning,sterilization and maintenance of ventilators,identification and treatment of ventilator alarms and hospital-acquired infection prevention and control.Results The results showed 20.4％of the respondents were nurses from critical care departments,and 79.6％of the respondents underwent the training for using the ventilator in their department;91.9％of the respondents could conduct ventilator self-testing by connecting the simulated lungs after the ventilator was switched on;66.4％of the respondents indicated that the initial parameters of the ventilator were set by the doctor;60.9％of the respondents proved that the performance monitoring and routine maintenance of the ventilator were carried out by the nurse;63.4％of the respondents showed disposable lines were commonly used in the departments.There were 89.8％of the respondents said the daily sterilization and management of the ventilator were performed by the nurse;46.0％of the respondents expressed the external surface of the ventilator was disinfected mainly with gamma disinfectant wipes;44.7％of the respondents indicated the external surface of the ventilator was sterilized every day;48.5％of the respondents said the internal airway of the ventilator was disinfected;57.0％of the respondents proved the disinfection was conducted after the expiratory flow sensors were used by any patient.There were 74.5％of the respondents that had paid attention to the ventilator waveform,and by the ventilator waveform only 21.3％were correct in determining whether there was secretion or fluid in the circuit and 22.1％in clarifying whether there was air leakage in the circuit.There were 59.1％of the respondents indicated that the closed suction tube was replaced once every 24 h;71.1％of the respondents could perform airbag pressure monitoring by the special pressure gauge.Conclusion Most of the nurses from secondary and tertiary medical institutions in Sichuan Province can use ventilators correctly,who have problems in disinfection specifications,infection prevention and control or recognition of ventilation waveform.It's suggested the training be strengthened to enhance the clinical nursing staffs in ventilator management.[Chinese Medical Equipment Journal,2025,46(3):75-80]

Hypertension is a cardiovascular syndrome characterized by increased systemic arterial blood pressure,which seriously affects human health and quality of life.At present,Chinese medicine has attracted much attention in the prevention and treatment of hypertension due to its multi-target and multi-pathway treatment advantages.Zhengan Xifeng decoction which has the effects of calming the liver and extinguishing wind,nourishing yin and suppressing yang.Zhengan Xifeng decoction has a good effect on hypertension,and its mechanism of action may be related to the regulation of intestinal flora,modulation of gastrointestinal hormones,blockade of renin-angiotensin-aldosterone system,inhibition of vascular remodeling,and improvement of insulin resistance.This paper systematically reviews the relevant research on Zhengan Xifeng decoction for the treatment of hypertension in recent years,in order to provide a reference for the further application of this prescription and subsequent related research.

Objective To observe the effects of Zhengan Xifeng decoction on bile acid-Takeda G protein-coupled receptor 5(TGR5)/Ras homolog(Rho)/Rho-associated coiled-coil containing protein kinase(ROCK)/agouti-related protein(AgRP)signaling pathway in spontaneously hypertensive rats(SHR).Methods Seventy SHR were randomly divided into the model group,control group(gavage administration of 0.5 mg·kg-1·d-1 amlodipine besylate tablets),experimental-L,-M,-H group(gavage administration of 8.630 x 103,1.725 × 104,3.450 × 104 mg·kg-1·d-1 Zhengan Xifeng decoction).Fifteen Wistar-Kyoto rats served as the normal group.The model and normal groups were given the same amount of distilled water.Six groups were treated for 8 weeks with once a day.The bile acid profiles of jejunum contents and colon contents were detected by ultra-high-performance liquid chromatography-tandem mass spectrometry,and the expression levels of TGR5,Rho,ROCK and AgRP proteins in the hypothalamus were detected by Western blot.Results Compared with the normal,mode,control groups and experimental-L,-M,-H groups,the bile acid spectrum of jejunum contents was significantly changed,and 6 different bile acids were found,namely glycoursodeoxycholic acid,glycohyodeoxycholic acid,hyodeoxycholic acid,ursodeoxycholic acid,taurochenodeoxycholic acid and chenodeoxycholic acid.The bile acid spectrum of colon contents was significantly changed,and 4 different bile acids were found,namely deoxycholic acid,ursodeoxycholic acid,lithocholic acid and hyodeoxycholic acid.The relative expression levels of TGR5 protein in normal,model,control and experimental-M groups were 1.01±0.22,0.75±0.23,0.96±0.16 and 1.02±0.21;the relative expression levels of Rho protein were 0.81±0.14,0.58±0.19,0.83±0.27 and 0.94±0.24;the relative expression levels of ROCK protein were 0.99±0.27,0.65±0.13,1.07±0.22 and 1.06±0.24;the relative expression levels of AgRP protein were 0.98±0.33,1.46±0.45,0.83±0.25 and 0.95±0.33,respectively.Compared with the model group,the above indexes in the experimental-M group and the normal group showed significant differences(all P＜0.05).Conclusion Zhengan Xifeng decoction may suppress AgRP expression via bile acid-TGR5/Rho/ROCK/AgRP signaling pathway,to achieve the purpose of reducing blood pressure.

Hypertension and osteoporosis(OP)are common diseases in middle-aged and elderly people,and the number of patients with both diseases has gradually increased in recent years.Because the onset of the disease is hidden,it is easy to cause fractures and serious complications of heart,brain and kidney in the later stage,which not only seriously damages the quality of life of patients,but also increases the difficulty of clinical treatment.Therefore,it is particularly necessary to strengthen the research on this disease.More and more studies have found that the disorder of intestinal flora will lead to the occurrence of OP,while the intestinal flora of patients with hypertension is obviously out of balance.Therefore,this paper thinks that intestinal flora may be the key influencing factor of hypertension complicated with OP,and the imbalance of intestinal flora will lead to the imbalance of short-chain fatty acid metabolism,immune inflammatory reaction and increased sympathetic nerve activity,thus causing the imbalance of bone homeostasis and promoting the occurrence of OP.Therefore,it is suggested that regulating intestinal flora may be a new way to intervene hypertension complicated with OP.

Hypertension is a cardiovascular syndrome characterized by increased systemic arterial blood pressure,which seriously affects human health and quality of life.At present,Chinese medicine has attracted much attention in the prevention and treatment of hypertension due to its multi-target and multi-pathway treatment advantages.Zhengan Xifeng decoction which has the effects of calming the liver and extinguishing wind,nourishing yin and suppressing yang.Zhengan Xifeng decoction has a good effect on hypertension,and its mechanism of action may be related to the regulation of intestinal flora,modulation of gastrointestinal hormones,blockade of renin-angiotensin-aldosterone system,inhibition of vascular remodeling,and improvement of insulin resistance.This paper systematically reviews the relevant research on Zhengan Xifeng decoction for the treatment of hypertension in recent years,in order to provide a reference for the further application of this prescription and subsequent related research.

Objective To observe the effects of Zhengan Xifeng decoction on bile acid-Takeda G protein-coupled receptor 5(TGR5)/Ras homolog(Rho)/Rho-associated coiled-coil containing protein kinase(ROCK)/agouti-related protein(AgRP)signaling pathway in spontaneously hypertensive rats(SHR).Methods Seventy SHR were randomly divided into the model group,control group(gavage administration of 0.5 mg·kg-1·d-1 amlodipine besylate tablets),experimental-L,-M,-H group(gavage administration of 8.630 x 103,1.725 × 104,3.450 × 104 mg·kg-1·d-1 Zhengan Xifeng decoction).Fifteen Wistar-Kyoto rats served as the normal group.The model and normal groups were given the same amount of distilled water.Six groups were treated for 8 weeks with once a day.The bile acid profiles of jejunum contents and colon contents were detected by ultra-high-performance liquid chromatography-tandem mass spectrometry,and the expression levels of TGR5,Rho,ROCK and AgRP proteins in the hypothalamus were detected by Western blot.Results Compared with the normal,mode,control groups and experimental-L,-M,-H groups,the bile acid spectrum of jejunum contents was significantly changed,and 6 different bile acids were found,namely glycoursodeoxycholic acid,glycohyodeoxycholic acid,hyodeoxycholic acid,ursodeoxycholic acid,taurochenodeoxycholic acid and chenodeoxycholic acid.The bile acid spectrum of colon contents was significantly changed,and 4 different bile acids were found,namely deoxycholic acid,ursodeoxycholic acid,lithocholic acid and hyodeoxycholic acid.The relative expression levels of TGR5 protein in normal,model,control and experimental-M groups were 1.01±0.22,0.75±0.23,0.96±0.16 and 1.02±0.21;the relative expression levels of Rho protein were 0.81±0.14,0.58±0.19,0.83±0.27 and 0.94±0.24;the relative expression levels of ROCK protein were 0.99±0.27,0.65±0.13,1.07±0.22 and 1.06±0.24;the relative expression levels of AgRP protein were 0.98±0.33,1.46±0.45,0.83±0.25 and 0.95±0.33,respectively.Compared with the model group,the above indexes in the experimental-M group and the normal group showed significant differences(all P＜0.05).Conclusion Zhengan Xifeng decoction may suppress AgRP expression via bile acid-TGR5/Rho/ROCK/AgRP signaling pathway,to achieve the purpose of reducing blood pressure.

OBJECTIVE: To investigate the effects of methionine restriction on proliferation, cell cycle and apoptosis of human acute leukemia cells. METHODS: Cell Counting Kit-8 (CCK-8) assay was used to detect the effect of methionine restriction on HL-60 and Jurkat cells proliferation. The effect of methionine restriction on cell cycle of HL-60 and Jurkat cells was examined by PI staining. Annexin V-FITC / PI double staining was applied to detect apoptosis of HL-60 and Jurkat cells following methionine restriction. The expression of cell cycle-related proteins cyclin B1, CDC2 and apoptosis-related protein Bcl-2 was evaluated by Western blot assay. RESULTS: Methionine restriction significantly inhibited the proliferation of HL-60 and Jurkat cells in a time-dependent manner (HL-60: r =0.7773, Jurkat: r =0.8725), arrested the cells at G2/M phase (P < 0.001), and significantly induced apoptosis of HL-60 and Jurkat cells (HL-60: P < 0.001; Jurkat: P < 0.05). Furthermore, Western blot analysis demonstrated that methionine restriction significantly reduced the proteins expression of Cyclin B1 (P < 0.05), CDC2 (P < 0.01) and Bcl-2 (P < 0.001) in HL-60 and Jurkat cells. CONCLUSION Acute leukemia cells HL-60 and Jurkat exhibit methionine dependence. Methionine restriction can significantly inhibit the proliferation, promote cell cycle arrest and induce apoptosis of HL-60 and Jurkat cells, which suggests that methionine restriction may be a potential therapeutic strategy for acute leukemia.
Humans ; Cyclin B1/pharmacology* ; Cell Proliferation ; Methionine/pharmacology* ; Cell Cycle ; Apoptosis ; Leukemia, Myeloid, Acute ; Cell Division ; Cell Cycle Proteins ; Jurkat Cells ; Proto-Oncogene Proteins c-bcl-2/metabolism* ; HL-60 Cells

This study was conducted to evaluate the efficacy and safety of Simotang Oral Liquid in the treatment of functional dyspepsia in adults. "Simotang Oral Liquid" "Simotang" "Si Mo Tang" "Si Mo Tang Oral Liquid" were used for retrieval of the relevant papers from CNKI, Wanfang, VIP, SinoMed, PubMed, Cochrane Library, Springer Link, and Web of Science from database inception to June 2021. Randomized controlled trial(RCT) of Simotang Oral Liquid in the treatment of functional dyspepsia in adults was screened out for Meta-analysis which was conducted in RevMan 5.3. A total of 16 RCTs were included. Meta-analysis showed that compared with the control group, Simotang Oral Liquid increased the total response rate and lowered the traditional Chinese medicine syndrome scores, serum cholecystokinin(CCK), serum nitric oxide(NO), and incidence of adverse reactions. However, the serum substance P(SP) had no statistical difference between the two groups. Simotang Oral Liquid is effective and safe in the treatment of functional dyspepsia in adults. However, this study has evidence and limitations, so the conclusions need to be further verified by large sample and multicenter clinical studies.
Adult ; Humans ; Databases, Factual ; Drugs, Chinese Herbal/therapeutic use* ; Dyspepsia/drug therapy* ; Medicine, Chinese Traditional ; Multicenter Studies as Topic ; Randomized Controlled Trials as Topic